Isoliquiritigenin induces oxidative stress and immune response in zebrafish embryos

Su, Yufang; Fang, Lei; Zhong, Kaili; Wang, Ting; Bao, Mingjie; Zhou, Ting; Zhu, Yuanchang

doi:10.1002/tox.23715

Cited by 11 publications

(5 citation statements)

References 44 publications

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“…Disruption of the redox state is crucial for maintaining proper protein folding within the endoplasmic reticulum, while excessive ROS can damage this redox state leading to misfolding or accumulation of folded proteins (Ly LD et al, 2017). Our previous research has shown that ISL promotes ROS production as an indicator of oxidative stress (Su Y and Fang L et al, 2023). Therefore, we hypothesize that endoplasmic reticulum stress may be one important mechanism underlying ISL-induced embryonic developmental disorders in zebra sh.…”

Section: Discussionmentioning

confidence: 87%

“…Additionally, ISL triggers developmental toxicity and oxidative stress-induced apoptosis in zebra sh embryos/larvae through the nRF2-HO1/JNK-ERK/mitochondrial pathway (Song Z and Zhang Y et al, 2020). Our previous research has also indicated that ISL disrupts immune system function by promoting the expression of proin ammatory factors (Su Y and Fang L et al, 2023), suggesting potential health risks associated with ISL exposure. However, it remains unclear whether ISL induces endoplasmic reticulum stress.…”

Section: Introductionmentioning

confidence: 97%

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Isoliquiritigenin induced endoplasmic reticulum stress in zebrafish embryos

Hu,

Xia,

Zhang

et al. 2024

Preprint

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Isoliquiritigenin (ISL), a natural flavonoid compound from the root of licorice, has antioxidant, anticancer, anti-inflammatory, and anti-allergic effects, and is commonly detected in both environmental and human samples. Our previous studies have demonstrated that exposure to ISL can result in developmental deformities and abnormal immune responses. However, the molecular mechanism underlying the toxicity of ISL towards zebrafish embryos remains incompletely understood. Therefore, this study aimed to investigate the effects of ISL exposure on endoplasmic reticulum (ER) stress in zebrafish embryos by examining the expression levels of ER stress markers HSPA5 and CHOP as well as related apoptosis factor under different concentrations of ISL, using Tm as a positive control group. Additionally, we performed targeted analysis of ER stress-related pathways via RNA transcriptome sequencing. The results showed that exposure to ISL induced high expression levels of HSPA5 and CHOP while activating ER stress pPERK-PEIF2a-ATF4 and ATF6 pathways in zebrafish embryos. These findings suggest that activation of endoplasmic reticulum stress signaling pathway may contribute to the developmental deformities observed following exposure to ISL in zebrafish embryos, highlighting potential ecological risks associated with its use.

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Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 97%

Isoliquiritigenin induced endoplasmic reticulum stress in zebrafish embryos

Hu,

Xia,

Zhang

et al. 2024

Preprint

Self Cite

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“…Table 1 shows the structure of the main saponins and flavonoid of Astragalus and its antioxidant effects. improved the activities of SOD, CAT, GSH-Px and inhibited the generation of ROS kidney protection [72], liver protection [73], anti-inflammatory [74], antiviral [75], immune regulation [76], liver protection [77], heart protection [78] [43]…”

Section: Biology Of Astragali Radix Extract and Its Components: The M...mentioning

confidence: 99%

“…Free radicals are produced by both endogenous and exogenous pathways. Endoge nous free radicals are the products of aerobic cell metabolism, and exogenous free radical improved the activities of SOD, CAT, GSH-Px and inhibited the generation of ROS kidney protection [72], liver protection [73], anti-inflammatory [74], antiviral [75], immune regulation [76], liver protection [77], heart protection [78] [43]…”

Section: Direct Scavenging Free Radicals In Vitromentioning

confidence: 99%

The Antioxidant Action of Astragali radix: Its Active Components and Molecular Basis

Yao,

Peng,

Shao

et al. 2024

Molecules

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Astragali radix is a traditional medicinal herb with a long history and wide application. It is frequently used in prescriptions with other medicinal materials to replenish Qi. According to the classics of traditional Chinese medicine, Astragali radix is attributed with properties such as Qi replenishing and surface solidifying, sore healing and muscle generating, and inducing diuresis to reduce edema. Modern pharmacological studies have demonstrated that some extracts and active ingredients in Astragali radix function as antioxidants. The polysaccharides, saponins, and flavonoids in Astragali radix offer beneficial effects in preventing and controlling diseases caused by oxidative stress. However, there is still a lack of comprehensive research on the effective components and molecular mechanisms through which Astragali radix exerts antioxidant activity. In this paper, we review the active components with antioxidant effects in Astragali radix; summarize the content, bioavailability, and antioxidant mechanisms; and offer a reference for the clinical application of Astragalus and the future development of novel antioxidants.

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“…Its dry roots and rhizomes have the functions of replenishing vital energy and blood, regulating the spleen, removing heat toxicity in the body, alleviating excessive medicinal properties of other drugs, resolving phlegm and relieving cough, maintaining healthy digestive functioning of the stomach, and improving digestive and absorption ability [8,9]. Glycyrrhiza radix contains more than 20 kinds of triterpenoids and more than 300 kinds of flavonoids [10], among which the bioactive substances with anti-inflammatory and antioxidant properties mainly comprise flavones, coumarone, isoflavones, liquiritigenin, glycyrrhizin, and chalcone [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Effects of Crude Extract of Glycyrrhiza Radix and Atractylodes macrocephala on Immune and Antioxidant Capacity of SPF White Leghorn Chickens in an Oxidative Stress Model

Zhang,

Wang,

Han

et al. 2024

Antioxidants

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The natural edible characteristics of Chinese herbs have led more and more people to study them as an alternative product to antibiotics. In this study, crude extracts of Glycyrrhiza radix and Atractylodes macrocephala (abbreviated as GRAM) with glycyrrhizic acid content not less than 0.2 mg/g were selected to evaluate the effects of GRAM on the immune and antioxidant capacity of model animals. Thirty 21-day-old male Leghorn chickens were weighed and randomly assigned to one of three groups of ten animals each. The treatments comprised a control group (CON), in which saline was injected at day 31, day 33, and day 35, an LPS-treated group (LPS), in which LPS (0.5 mg/kg of BW) was injected at day 31, day 33, and day 35, and finally a GRAM and LPS-treated group, (G-L) in which a GRAM-treated diet (at GRAM 2 g/kg) was fed from day 21 to day 35 with LPS injection (0.5 mg/kg of BW) at day 31, day 33, and day 35. The results of diarrhea grade and serum antioxidant measurement showed that the LPS group had obvious diarrhea symptoms, serum ROS and MDA were significantly increased, and T-AOC was significantly decreased. The oxidative stress model of LPS was successfully established. The results of immune and antioxidant indexes showed that feeding GRAM significantly decreased levels of the pro-inflammatory factors TNF-α, IL-1β, and IL-6 (p < 0.05) and significantly increased levels of the anti-inflammatory factors IL-4 and IL-10 and levels of the antioxidant enzymes GSH-Px and CAT (p < 0.05). GRAM resisted the influence of LPS on ileum morphology, liver, and immune organs and maintained normal index values for ileum morphology, liver, and immune organs. In summary, this study confirmed the antidiarrheal effect of GRAM, which improved the immune and antioxidant capacity of model animals by regulating inflammatory cytokine levels and antioxidant enzyme activity in poultry.

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Isoliquiritigenin induces oxidative stress and immune response in zebrafish embryos

Cited by 11 publications

References 44 publications

Isoliquiritigenin induced endoplasmic reticulum stress in zebrafish embryos

Isoliquiritigenin induced endoplasmic reticulum stress in zebrafish embryos

The Antioxidant Action of Astragali radix: Its Active Components and Molecular Basis

Effects of Crude Extract of Glycyrrhiza Radix and Atractylodes macrocephala on Immune and Antioxidant Capacity of SPF White Leghorn Chickens in an Oxidative Stress Model

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