Isoliquiritigenin Reduces LPS-Induced Inflammation by Preventing Mitochondrial Fission in BV-2 Microglial Cells

Lee, Dong-Seok; Nam, Bo Ra; Huh, Jae‐Won

doi:10.1007/s10753-020-01370-2

Cited by 20 publications

(9 citation statements)

References 47 publications

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“…The anti-inflammatory activity of the extracts was evaluated pretreating BV-2 cells with different concentrations (1-100 μ g mL −1 ) of the extracts for 24 h before exposing the cells to lipopolysaccharide (LPS) to induce inflammation ( Figure 8 ). LPS is the most widely used inflammatory mediator to activate microglial cells in vitro and triggers the proinflammatory signaling cascade [ 78 , 79 ]. LPS treatment significantly reduced cell viability with respect to control cells by ~40%.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant and Anti‐Inflammatory Profiles of Spent Coffee Ground Extracts for the Treatment of Neurodegeneration

Angeloni

Freschi

Marrazzo

et al. 2021

Oxidative Medicine and Cellular Longevity

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Spent coffee grounds (SCGs), waste products of coffee beverage production, are rich in organic compounds such as phenols. Different studies have demonstrated phenol beneficial effects in counteracting neurodegenerative diseases. These diseases are associated with oxidative stress and neuroinflammation, which initiates the degeneration of neurons by overactivating microglia. Unfortunately, to date, there are no pharmacological therapies to treat these pathologies. The aim of this study was to evaluate the phenolic content of 4 different SCG extracts and their ability to counteract oxidative stress and neuroinflammation. Caffeine and 5-O-caffeoylquinic acid were the most abundant compounds in all extracts, followed by 3-O-caffeoylquinic acid and 3,5-O-dicaffeoylquinic acid. The four extracts demonstrated a different ability to counteract oxidative stress and neuroinflammation in vitro. In particular, the methanol extract was the most effective in protecting neuron-like SH-SY5Y cells against H2O2-induced oxidative stress by upregulating endogenous antioxidant enzymes such as thioredoxin reductase, heme oxygenase 1, NADPH quinone oxidoreductase, and glutathione reductase. The water extract was the most effective in counteracting lipopolysaccharide-induced neuroinflammation in microglial BV-2 cells by strongly reducing the expression of proinflammatory mediators through the modulation of the TLR4/NF-κB pathway. On these bases, SCG extracts could represent valuable nutraceutical sources for the treatment of neurodegeneration.

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Section: Resultsmentioning

confidence: 99%

Antioxidant and Anti‐Inflammatory Profiles of Spent Coffee Ground Extracts for the Treatment of Neurodegeneration

Angeloni

Freschi

Marrazzo

et al. 2021

Oxidative Medicine and Cellular Longevity

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“…Lianqiao (Fructus Forsythiae), Fangfeng (Radix Saposhnikoviae divaricatae), Shanyinchaihu (Gypsophila pacifica), Mabiancao (Verbena officinalis), Huoxiang (Agastache rugosus), Baishu (Rhizoma Atractylodis macrocephalae), Huangqi (Radix Astragali), and Gancao (Radix Glycyrrhizae) are the main herbal ingredients of Ganweikang tablet. Previous studies have revealed that these herbals formulating Ganweikang tablet can attenuate inflammation ( Zhou et al, 2019 ; Lee et al, 2021 ), liver injury ( Hu et al, 2020 ), and apoptosis ( Fattorusso et al, 2006 ; Hu et al, 2014 ; Adesso et al, 2018 ; Lee et al, 2021 ; Zhao et al, 2021 ), which indicates the potential and beneficial effects of Ganweikang tablet on NAFLD. However, whether it can alleviate NAFLD, especially the NAFL and NASH, remains unclear.…”

Section: Introductionmentioning

confidence: 94%

Exploring Ganweikang Tablet as a Candidate Drug for NAFLD Through Network Pharmacology Analysis and Experimental Validation

Wang²,

Zhang³

et al. 2022

Front. Pharmacol.

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Nonalcoholic fatty liver disease (NAFLD) is defined as liver disease in which more than 5% of hepatocytes are steatotic with little or no alcohol consumption. NAFLD includes benign nonalcoholic fatty liver (NAFL) and nonalcoholic steatohepatitis (NASH). Importantly, NASH is an advanced progression of NAFL and is characterized by steatosis, hepatocyte ballooning, lobular inflammation, and fibrosis. However, to date, no drugs specifically targeting NAFLD have been approved by the FDA. Therefore, a new drug or strategy for NAFLD treatment is necessary. However, the pathogenesis of NAFLD is complex and no single-target drugs have achieved the desired results. Noticeably, traditional Chinese medicine formulations are a complex system with multiple components, multiple targets, and synergistic effects between components. The Ganweikang tablet is a compound formula based on traditional Chinese medicine theory and clinical experience. In this study, network pharmacology analysis indicates Ganweikang tablet as a candidate for NAFLD treatment. Furthermore, we evaluated the therapeutic effects of Ganweikang tablet on the NAFL and NASH and tried to clarify the underlying molecular mechanisms in animal models and cell experiments. As expected, Ganweikang tablet was found to improve NAFL and NASH by modulating inflammation, apoptosis, and fatty acid oxidation by inhibiting NFκB, caspase-8, and activating PPARα, which not only indicates that Ganweikang tablet as a drug candidate but also provides a theoretical basis of Ganweikang tablet for the treatment of NAFL and NASH.

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“…Only rare side effects like minor hematological, gastrointestinal, and cardiovascular adverse effects have been shown in experimental animal studies (Olson et al, 2000). Interestingly, previous studies showed an excellent protective effect of natural chalcones against I/R-induced tissue injury (Hu et al, 2018;Jiao et al, 2020;Lee et al, 2020;Si et al, 2018;Wang et al, 2020;Wei et al, 2018;You et al, 2018;Zhou et al, 2019). In this review, we studied 12 members of natural chalcones (Figure 2) and their impacts on different I/R conditions to propose the underlying possible therapeutic mechanisms based on the previously published reports (Table 1).…”

Section: Effec T Of Natur Al Chalcone S On D Ifferent T Ype S Of I/r ...mentioning

confidence: 99%

“…Isoliquiritigenin (ISL) is a simple chalcone derived from licorice and widely distributed in foods, beverages, and tobacco. Like other bioactive chalcones, ISL exhibits a potent antioxidant (Zhang et al, 2010), anti-inflammatory (Lee et al, 2020;Tang et al, 2018), antiplatelet aggregation (Tawata et al, 1992), and antitumor (Hsu et al, 2004(Hsu et al, , 2005Jung et al, 2006) activities. Besides these properties, ISL demonstrates excellent protective effects against I/R injury.…”

Section: Isoliquiritigeninmentioning

confidence: 99%

The importance of natural chalcones in ischemic organ damage: Comprehensive and bioinformatic analysis review

Sayed

Gohar

Abd-alhameed

et al. 2022

Journal of Food Biochemistry

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Over the last few decades, extensive research has been conducted, yielding a detailed account of thousands of newly discovered compounds of natural origin and their biological activities, all of which have the potential to be used for a wide range of therapeutic purposes. There are multiple research papers denoting the central objective of chalcones, which have been shown to have therapeutic potential against various forms of ischemia. The various aspects of chalcones are discussed in this review regarding molecular mechanisms involved in the promising anti‐ischemic potential of these chalcones. The main mechanisms involved in these protective effects are Nrf2/Akt activation and NF‐κB/TLR4 suppression. Furthermore, in‐silico studies were carried out to discover the probable binding of these chalcones to Keap‐1 (an inhibitor of Nrf2), Akt, NF‐κB, and TLR4 protein molecules. Besides, network pharmacology analysis was conducted to predict the interacting partners of these signals. The obtained results indicated that Nrf2, Akt, NF‐κB, and TLR4 are involved in the beneficial anti‐ischemic actions of chalcones. Conclusively, the present findings show that chalcones as anti‐ischemic agents have a valid rationale. The discussed studies will provide a comprehensive viewpoint on chalcones and can help to optimize their effects in different ischemia. Practical applications Ischemic organ damage is an unavoidable pathological condition with a high worldwide incidence. According to the current research progress, natural chalcones have been proved to treat and/or prevent various types of ischemic organ damage by alleviating oxidative stress, inflammation, and apoptosis by different molecular mechanisms. This article displays the comprehensive research progress and the molecular basis of ischemic organ damage pathophysiology and introduces natural chalcones' mechanism in the ischemic organ condition.

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Isoliquiritigenin Reduces LPS-Induced Inflammation by Preventing Mitochondrial Fission in BV-2 Microglial Cells

Cited by 20 publications

References 47 publications

Antioxidant and Anti‐Inflammatory Profiles of Spent Coffee Ground Extracts for the Treatment of Neurodegeneration

Antioxidant and Anti‐Inflammatory Profiles of Spent Coffee Ground Extracts for the Treatment of Neurodegeneration

Exploring Ganweikang Tablet as a Candidate Drug for NAFLD Through Network Pharmacology Analysis and Experimental Validation

The importance of natural chalcones in ischemic organ damage: Comprehensive and bioinformatic analysis review

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