Isomer selectivity of one- and two-dimensional approaches of mixed-mode and hydrophilic interaction liquid chromatography coupled to tandem mass spectrometry for sugar phosphates of glycolysis and pentose phosphate pathways

Su, Min; Serafimov, Kristian; Knappe, Cornelius; Lämmerhofer, Michael

doi:10.1016/j.chroma.2022.463727

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Cited by 10 publications

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“…In addition, a study under high pH conditions by hydrophilic interaction liquid chromatography-mass spectrometry (HILIC-MS) showed that these conditions are suitable for separating isomers of some sugar phosphates. 18 Herein, we aimed to develop a simple method for the separation of UDP-HexNAcs by HILIC-MS. We performed preliminary experiments and found that 20 mM ammonium bicarbonate or 0.1% ammonia (NH 3 ) in a mobile phase provided a high two-peak resolution. Since less reproducibility was observed when ammonium bicarbonate was used, we selected NH 3 as the mobile phase additive.…”

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confidence: 99%