Isomerization and increase in the antioxidant properties of lycopene from Momordica cochinchinensis (gac) by moderate heat treatment with UV–Vis spectra as a marker

Phan-Thi, Hanh; Waché, Yves

doi:10.1016/j.foodchem.2014.01.040

Cited by 40 publications

(27 citation statements)

References 36 publications

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“…Although, future research will be required on MCS extract, we do know that the seeds are orange/red in color due to lycopene [59, 60] and it has significant concentrations of triterpenoidal saponins, gypsogenin and quillaic acid glycosides [61] and low MW cell penetrating dipeptides [62] such as cochinin B (28 kDa) [63] and M. cochinchinensis trypsin inhibitor I (MCoTI-I) and 2 (MCoTI-II). [64] MCoTI-II belongs to the cyclotide family of plant-derived cyclic peptides that are characterized by a cyclic cystine knot motif [65, 66] known to be thermally and chemically stable, and resistant to enzymatic degradation.…”

Section: Discussionmentioning

confidence: 99%

Neurotrophic Effects of Mu Bie Zi (Momordica cochinchinensis) Seed Elucidated by High-Throughput Screening of Natural Products for NGF Mimetic Effects in PC-12 Cells

et al. 2015

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Post-mitotic central nervous system (CNS) neurons have limited capacity for regeneration, creating a challenge in the development of effective therapeutics for spinal cord injury or neurodegenerative diseases. Furthermore, therapeutic use of human neurotrophic agents such as nerve growth factor (NGF) are limited due to hampered transport across the blood brain barrier (BBB) and a large number of peripheral side effects (e.g. neuro-inflammatory pain/tissue degeneration etc.). Therefore, there is a continued need for discovery of small molecule NGF mimetics that can penetrate the BBB and initiate CNS neuronal outgrowth/regeneration. In the current study, we conduct an exploratory high-through-put (HTP) screening of 1144 predominantly natural/herb products (947 natural herbs/plants/spices, 29 polyphenolics and 168 synthetic drugs) for ability to induce neurite outgrowth in PC12 dopaminergic cells grown on rat tail collagen, over 7 days. The data indicate a remarkably rare event-low hit ratio with only 1/1144 tested substances (<111.25 µg/mL) being capable of inducing neurite outgrowth in a dose dependent manner, identified as; Mu Bie Zi, Momordica cochinchinensis seed extract (MCS). To quantify the neurotrophic effects of MCS, 36 images (n = 6) (average of 340 cells per image), were numerically assessed for neurite length, neurite count/cell and min/max neurite length in microns (µm) using Image J software. The data show neurite elongation from 0.07 ± 0.02 µm (controls) to 5.5 ± 0.62 µm (NGF 0.5 μg/mL) and 3.39 ± 0.45 µm (138 μg/mL) in MCS, where the average maximum length per group extended from 3.58 ± 0.42 µm (controls) to 41.93 ± 3.14 µm (NGF) and 40.20 ± 2.72 µm (MCS). Imaging analysis using immunocytochemistry (ICC) confirmed that NGF and MCS had similar influence on 3-D orientation/expression of 160/200 kD neurofilament, tubulin and F-actin. These latent changes were associated with early rise in phosphorylated extracellular signal-regulated kinase (ERK) p-Erk1 (T202/Y204)/p-Erk2 (T185/Y187) at 60 min with mild changes in pAKT peaking at 5 min, and no indication of pMEK involvement. These findings demonstrate a remarkable infrequency of natural products or polyphenolic constituents to exert neurotrophic effects at low concentrations, and elucidate a unique property of MCS extract to do so. Future research will be required to delineate in depth mechanism of action of MCS, constituents responsible and potential for therapeutic application in CNS degenerative disease or injury.

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Section: Discussionmentioning

confidence: 99%

Neurotrophic Effects of Mu Bie Zi (Momordica cochinchinensis) Seed Elucidated by High-Throughput Screening of Natural Products for NGF Mimetic Effects in PC-12 Cells

et al. 2015

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“…Phan-Thi and Wache [72] studied the effect of heat treatment on lycopene concentration and antioxidant ability. They found that moderate heating transferred all trans-isomers to cis-isomers leading to increased antioxidant activity.…”

Section: Antioxidant Activitymentioning

confidence: 99%

Food prospects and nutraceutical attributes of Momordica species: A potential tropical bioresources – A review

Nagarani

Abirami

Siddhuraju

2014

Food Science and Human Wellness

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Plants with potential therapeutic values have been used from time immemorial to cure various ailments and infectious diseases. Of late, scientific evidences have been provided on the potential therapeutic agent exhibited by certain traditionally used vegetable extracts. The importance of wild edible plants may be traced to antiquity but systemic studies are recent. All the Momordica species have been consumed as vegetable and traditionally used for various disorders. The whole plant parts are ascribed to possess the anti-diabetic effect in traditional medicinal system. The active constituents of Momordica plant parts were cucurbitane type triterpenoids, phenolics, glycosides, and several kinds of peptides including Momordica anti-HIV protein (MAP 30). Recent reports revealed the presence of several kinds of cucurbitane type triterpenoids in leaf, stem and fruits of Momordica species having several pharmacological activities. There is lack of scientific information available on the wild species which also having several bioactive components with potential activities. So the present review compares and highlights the current knowledge of the nutritional value, phytochemistry and physiological effects of wild species with known variety.

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“…[1] Lycopene is a natural carotenoid, [2] which shows powerful antioxidant properties, even higher than β-carotene. The fruit, guava, has originally come from Mexico and Central America, but it is also very common in Brazil.…”

Section: Introductionmentioning

confidence: 99%

“…[3] This bioactive compound has attracted considerable attention in the last two decades due to its protective effects against several chronic diseases, particularly skin cancer, [4,5] prostate cancer, lung cancer, [6] cardiovascular diseases, [5] and degenerative diseases. [2] However, these studies showed that there is a synergistic antioxidant activity of lycopene in combination with β-carotene and other carotenoids, such as lutein and β-cryptoxanthin, and this activity was more efficient in protecting liposome from oxidation than the individual carotenoid. [7] Although lycopene extracts from tomato are the most studied and used according to the literature, it is worth pointing out that lycopene extract from guava is also of great biotechnological interest, once that lycopene and other carotenoids with antioxidant potential, such as β-carotene, are present in this fruit.…”

Section: Introductionmentioning

confidence: 99%

HPLC‐DAD, ESI–MS/MS, and NMR of Lycopene Isolated From P. guajava L. and Its Biotechnological Applications

Amorim

Souza

Santos

et al. 2018

Euro J Lipid Sci & Tech

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Psidium guajava L. have been reported to be a rich source of antioxidant compounds. Its carotenoids have been highlighted by their high antioxidant capacity, which offers several benefits for human health. In this sense, lycopene isomers need to be identified. In this work, the comprehensive chemical characterization, by HPLC‐DAD, MS/MS, and NMR, of lycopene isolated from P. guajava L., antioxidant and antimicrobial activity of lycopene extracts and isolated lycopene are evaluated. The FTIR results reported a structure with Z configuration, confirmed by UV‐Vis, with λmax = 448, 473, and 505 nm for 5‐Z lycopene. Furthermore, MS/MS positive ionization shows one fragment m/z 309 [M‐227]+, relatively abundant for isolated lycopene. Experimental and Theoretical NMR studies revealed that guava may contain 5‐Z lycopene because of the similarity found among the peaks. Lycopene extracts presented higher antioxidant activity than isolated lycopene, from both P. guajava L. and tomato, when measured by ABTS and ORAC (r2 = 0.9995 and r2 = 0.9992, respectively). In addition, lycopene extract shows antibacterial efficacy against E. coli, S. aureus, and L. innocua, presenting MBC values of 20 mg mL−1. These results suggest that lycopene extract have potential applicability for food, cosmetics, and pharmaceutical industry. Practical Applications: Lycopene from P. guajava L. is characterized by HPLC‐DAD, MS, NMR, FTIR, and X‐Ray, presented antioxidant capacity by ORAC and antibacterial efficacy against food pathogens. Lycopene is isolated from P. guajava L., and lycopene extracts produced are characterized by HPLC‐DAD and ESI‐MS/MS. Lycopene isolated from P. guajava L. following the process may be 5‐Z isomer of lycopene.

show abstract

Isomerization and increase in the antioxidant properties of lycopene from Momordica cochinchinensis (gac) by moderate heat treatment with UV–Vis spectra as a marker

Cited by 40 publications

References 36 publications

Neurotrophic Effects of Mu Bie Zi (Momordica cochinchinensis) Seed Elucidated by High-Throughput Screening of Natural Products for NGF Mimetic Effects in PC-12 Cells

Neurotrophic Effects of Mu Bie Zi (Momordica cochinchinensis) Seed Elucidated by High-Throughput Screening of Natural Products for NGF Mimetic Effects in PC-12 Cells

Food prospects and nutraceutical attributes of Momordica species: A potential tropical bioresources – A review

HPLC‐DAD, ESI–MS/MS, and NMR of Lycopene Isolated From P. guajava L. and Its Biotechnological Applications

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