Isoniazid and rifampicin resistance-associated mutations in Mycobacterium tuberculosis isolates from Yangon, Myanmar: implications for rapid molecular testing

Abstract

This study provides the first molecular characterization of isoniazid- and rifampicin-resistant M. tuberculosis isolates from Myanmar and gives information on the molecular basis for rifampicin and isoniazid drug resistance in M. tuberculosis. The study generates useful information for the development of potential rapid molecular drug susceptibility tests.

Predicting Extensively Drug-Resistant Mycobacterium tuberculosis Phenotypes with Genetic Mutations

“…The combined sensitivity of katG and inhA promoter mutations for the detection of the phenotypic INH r that we observed (96%) is consistent with the cumulative frequency of these mutations in the published literature (24)(25)(26). There are, however, some recent studies on international isolates which suggest that using only katG and inhA promoter SNPs will yield sensitivities of Ͻ90% due to isolates that do not have these SNPs (27).…”

Molecular Characterization of Multidrug-Resistant Mycobacterium tuberculosis Isolated in Nepal

“…The occurrence of the KatG Ser315Thr alteration among Nepalese isolates was higher than that reported in India (16,22) and in China (4, 10, 12) ( Table 6). Van Soolingen et al (32) reported that strains with amino acid substitutions in katG 315 are more likely to develop resistance to (6,26,31). Several studies from different countries have shown that ca.…”

Genotyping and molecular characteristics of multidrug-resistant Mycobacterium tuberculosis isolates from China

“…13 For INH, several genes, including katG, the promoter of inhA and the intergenic region of oxyR-ahpC, are associated with INH resistance in M. tuberculosis isolates. 13,15 Of those INH resistance targets, the substitution of a single nucleotide at codon 315 of the katG gene is the most frequently identified mutation type, conferring approximately 70% of INH resistant isolates. 15,16 Due to the high prevalence of MDR among TB patients, China has been classified as global "hotspots" of MDR-TB.…”

“…13,15 Of those INH resistance targets, the substitution of a single nucleotide at codon 315 of the katG gene is the most frequently identified mutation type, conferring approximately 70% of INH resistant isolates. 15,16 Due to the high prevalence of MDR among TB patients, China has been classified as global "hotspots" of MDR-TB. 2,17,18 Although several molecular epidemiological studies have been performed among MDR strains isolated from different regions of China, the knowledge on molecular characteristics of MDR-TB isolates representative of the whole China is still unknown now.…”