Isoproterenol-induced Creatine Kinase Leakage in Langendorff-perfused Rat Heart Associated with Significant Myocardial Edema.

Arrhythmogenic and Inotropic Effects of Interferon Investigated in Perfused and in Vivo Rat Hearts. Influences of Cardiac Hypertrophy and Isoproterenol.

“…CK activity quantitated by time-sampled effluent in the perfusion study was less than 10 U/L in all the preparations, irrespective of the perfusion protocol, and we had found in our earlier perfusion study 17 that this range of CK leakage in the perfused rat hearts was indicative of no specific myocardial damage. LVDP showed a slight, but significant increase with 10 -9 mol/L isoproterenol in the control group (96±5 to 117±6 mmHg, n=4; p<0.05) (Fig 3), and in the other 2 groups (103±8 to 121±9 mmHg in the aorta-banded, n=5; 102±9 to 124±11 mmHg in the DOCA-salt group, n=5; p<0.05 in both analyses).…”

“…17,18 In brief, rats aged 15 weeks were anesthetized by an intraperitoneal injection of sodium pentobarbital (30-40 mg/kg; Abbott Laboratory, Chicago, IL, USA). The beating heart was removed immediately after thoracotomy under conditions of artificial respiration (KN-55, Natsume, Tokyo).…”

“…Some of the preparations in each group had pretreatment with isoproterenol (10 -9 mol/L; Sigma); the concentration of isoproterenol had previously been confirmed not to cause myocardial creatine kinase (CK) leakage or any specific histological alterations. 17 Effluent CK activity was estimated by a spectrophotometric kinetic procedure using a commercially available CK assay kit (Wako Chemical Co Ltd, Tokyo). After conducting the perfusion protocols, all the preparations were perfused with control K-H solution containing trypan blue (10mg/dl; Sigma) for 10 min, washed with Karnovsky's fixative, removed from the perfusion system, stored in the fixative for 48 h and then embedded in paraffin wax.…”