Isoproterenol Reduces Ischemia-Reperfusion Lung Injury Despite β-Blockade1

Takashima, S; Schlidt, Scott A.; Koukoulis, Giovanna; Sevala, Mayura; Egan, Thomas M.

doi:10.1016/j.jss.2005.01.017

Cited by 8 publications

(4 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although PC loss is one of the important features of DR and is presumed to be related to the initial onset of DR lesions, such as such as microaneurysm, vascular leakage, and proliferative neovascularization (43, 44), little is known about the direct causal relationship between PC loss and vascular leakage in DR. PC detachment from cerebral blood vessels is also known to impair barrier function (45), and PC loss in cardiac coronary vessels increases vascular permeability (46, 47). In addition, a growing body of evidence has shown that β‐agonists significantly reduce endothelial permeability in lung caused by multiple stimuli, such as inflammation (48), thrombin (49), and ischemia–reperfusion injury (50). These studies showed the effect of β‐AR stimulation on EC integrity, but the effect of β‐AR stimulation on PC survival and vascular leakage remained unknown.…”

Section: Discussionmentioning

confidence: 99%

β‐Adrenergic receptor agonists attenuate pericyte loss in diabetic retinas through Akt activation

et al. 2017

View full text Add to dashboard Cite

Pericytes (PCs) are crucial in maintaining the quiescence of endothelial cells (ECs) and the integrity of EC tight junctions. Especially in diabetic retinopathy (DR), PC loss is one of the early pathologic changes in capillaries of diabetic retinas. Thus, preventing PC loss is beneficial for attenuating vision impairment in patients with DR. Although many studies have revealed the mechanism of PC loss in retinas, little is known about the mechanisms that increase PC survival. We focused on the effect of β-adrenergic receptor agonists (β-agonists) on PC loss in diabetic retinas. In this study, β-agonists increased the cell viability of PCs by increasing PC survival and proliferation. Mechanistically, β-agonist-induced protein kinase B activation in PCs reduced PC apoptosis in response to various stimuli. β2-agonists more potently increased PC survival than β1-agonists. β2-Agonist reduced vascular leakage and PC loss in retinas of mice with streptozotocin-induced diabetes. In cocultures of PCs and ECs, β2-agonists restored the altered permeability and ZO-1 expression in ECs induced by PC loss. We concluded that β-agonists, especially β2-agonists, increase PC survival, thereby preventing diabetes-induced PC loss in retinas. These results provide a potential therapeutic benefit of β-agonists for preventing PC loss in DR.-Yun, J.-H., Jeong, H.-S., Kim, K.-J., Han, M. H., Lee, E. H., Lee, K., Cho, C.-H. β-Adrenergic receptor agonists attenuate pericyte loss in diabetic retinas through Akt activation.

show abstract

Section: Discussionmentioning

confidence: 99%

β‐Adrenergic receptor agonists attenuate pericyte loss in diabetic retinas through Akt activation

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Almost all of the studies on ischemia-reperfusion injury in NHBDs are focused on the changes occurring in the reperfusion phase while the inflammatory and cellular changes during the warm ischemic period remain poorly understood. [21][22][23][24] Geudens et al [7] showed that the cellular changes already occur during warm ischemia, and these were correlated with the length of the ischemic period in the mouse model. In this study, it was demonstrated that the number of macrophages and lymphocytes in bronchoalveolar lavage increases with longer warm ischemic intervals, and a significant rise occurs between 60 and 90 minutes of ischemia.…”

Section: Discussionmentioning

confidence: 99%

Better lung protection following death due to rapid exsanguination in rats

Bostancı¹

2012

Turk Gogus Kalp Dama

View full text Add to dashboard Cite

Bu çalışmada hızlı eksanguinasyon ile ölümün akciğer dokusu canlılığı üzerindeki etkisi araştırıldı. Ça lış ma pla nı: Çalışmaya ağırlıkları 310-370 g arasında değişen 46 adet Sprague-Dawley cinsi erkek sıçan alındı. Sıçanlar altı gruba ayrıldı; (i) sadece iskemide tutulan grup (I grubu; n=8), (ii) ölüm sonrası majör abdominal damarların kesildiği pasif eksanguinasyon grubu (PE grubu; n=8), (iii) sıçanların majör abdominal damarlar kesilerek hızlı eksanguinasyonla sakrifiye edildiği grup (RE grubu; n=8), (iv) akciğerlerin serum fizyolojikle perfüze edildiği grup (SP grubu; n=8), (v) akciğerlerin Perfadex ile perfüze edildiği grup (PP grubu; n=8) ve (vi) kontrol grubu (C grubu; n=6). Hızlı eksanguinasyon grubu dışındaki deney gruplarında ve kontrol grubundaki tüm sıçanlara intrahepatik pentobarbital ile ötenazi uygulandı. Kontrol grubunda ötenazi sonrası akciğerler çıkartıldı. Tüm çalışma gruplarında akciğerler ölü sıçanın vücudunda 120 dakika süreyle oda ısısında ventile edilerek sıcak iskemide tutuldu. Bul gu lar: Miyeloperoksidaz (MPO) aktivitesi, lüminol kemilüminesans (CL) değerleri ve ölü hücre oranları iskemi grubunda yüksek bulundu. İskemi grubuyla karşılaştırıldığında PE grubunda MPO aktivitesi, lüsigenin CL değerleri ve ölü hücre oranları yüksek iken, RE grubunda MPO aktivitesi ve lüminol CL değerleri düşük bulundu. PE grubu ile karşılaştırıldığında da RE grubunda MPO aktivitesi, lüsigenin CL değerleri ve ölü hücre oranları düşük bulundu. SP ve iskemi gruplarıyla karşılaştırıldığında, PP grubunda MPO aktivitesi ve lüminol CL değerleri düşük, ölü hücre oranları ise yüksek bulundu. So nuç: Hızlı eksanguinasyon ile ölümde akciğer canlılığı daha iyi korunur ve oksidatif hasar minimaldir. Bu durum trombositlerin ve inflamatuvar hücrelerin hızla dokudan uzaklaşması ve ekstravasküler sıvının intravasküler kompartmana kayması ile açıklanabilir. Anah tar söz cük ler: Kadavra akciğeri; donör akciğeri; iskemik hasar; akciğer transplantasyonu; organ korunması. Background:This study aims to investigate the effects of death due to rapid exsanguination on the viability of lung tissue. Methods: Fourty-six Sprague-Dawley male rats with a weight range of 310-370 g were included in the study. Rats were divided into six groups: (i) ischemic alone (I group; n=8); (ii) passive exsanguination group of whose major abdominal veins were cut following death (PE group; n=8) (iii) group of whose major abdominal veins were cut and sacrified with rapid exsanguination (RE group; n=8); (iv) lung perfusion group with saline (SP group; n=8); (v) lung perfusion group with Perfadex (PP group; n=8) and (vi) control group (C group; n=6). Rats in all experiement groups except rapid exsanguination ones and all in the control group were euthanized with intrahepatic pentobarbital. Lungs were removed following euthanasia in the controls. In all study groups, lungs were ventilated in the cadavers at room temperature for 120 minutes and kept in warm ischemia. Results: Myeloperoxidase (MPO) activity, luminol chemiluminescence (CL) values and non-viab...

show abstract

“…In addition, new evidences identified interesting properties of this drug. For instance, isoproterenol attenuates ischemiareperfusion injury even in the presence of beta-blockade (24).…”

Section: Discussionmentioning

confidence: 99%

A Reappraisal of Isoproterenol in Goal-Directed Therapy of Septic Shock

Léone¹,

Boyadjiev²,

Boulos³

et al. 2006

Shock

View full text Add to dashboard Cite

The goal of the study was to evaluate the effect of isoproterenol prescribed in goal-directed therapy for septic shock. Out of a cohort of 89 patients with septic shock, 14 patients treated with fluid and norepinephrine had inappropriate mixed venous oxygen saturation (SvO2<70%) not responding to correction of hypoxemia and anemia (>8 g.dL-1). Isoproterenol administration was started at a dose of 0.04 microg.kg-1.minute-1 with 0.025 microg.kg-1.minute-1 increments every 30 minutes until SvO2 was greater than 70%. Mean arterial pressure was maintained>or=65 mm.Hg by adjusting the norepinephrine infusion. Hemodynamic, oxygen, and renal variables were collected during a 12-h period. Patients with a known prior history of coronary disease were not eligible. Isoproterenol administration increased significantly SvO2 (62%+/-10% to 71%+/-9%), cardiac index (3.1+/-0.6 to 4.4+/-1.4 L.min-1.m-2), stroke index (27+/-3.4 to 38+/-6.1 mL.m-2), and left ventricular stroke work index (24+/-3.4 to 40+/-5.0 g.m-1.m-2). Heart rate rise did not reach a significant level. Arterial lactate concentration decreased significantly during the study period (5.7+/-2.8 to 3.4+/-1.6 mmol.L-1). No cardiac adverse events occurred with any electrocardiographic aspects of myocardial ischemia. This study suggests that isoproterenol is efficient to improve hemodynamics and oxygen variables in septic shock patients. There is a need for future investigations in larger groups of patients to determine whether isoproterenol can be an alternative to dobutamine.

show abstract

Isoproterenol Reduces Ischemia-Reperfusion Lung Injury Despite β-Blockade1

Cited by 8 publications

References 35 publications

β‐Adrenergic receptor agonists attenuate pericyte loss in diabetic retinas through Akt activation

β‐Adrenergic receptor agonists attenuate pericyte loss in diabetic retinas through Akt activation

Better lung protection following death due to rapid exsanguination in rats

A Reappraisal of Isoproterenol in Goal-Directed Therapy of Septic Shock

Contact Info

Product

Resources

About