Isorhamnetin attenuates TNF‐α‐induced inflammation, proliferation, and migration in human bronchial epithelial cells via MAPK and NF‐κB pathways

Abstract

Isorhamnetin has distinct anti‐inflammatory activity and inhibits cell proliferation and migration. These effects are also involved in the pathogenesis of asthma. However, the effect of isorhamnetin on bronchial epithelial cells in patients with asthma has not been examined. Cells of human bronchial epithelial cell line BEAS‐2B were cultured with isorhamnetin and tumor necrosis factor (TNF)‐α. The effects of isorhamnetin on BEAS‐2B cell viability were assessed using CCK8 assay. The EdU (5‐ethynyl‐2′‐deoxyuridine) cell proliferation assay was performed to assess cell proliferation. BEAS‐2B cell migration was measured using Transwell and wound healing assays. Real‐time PCR and enzyme‐linked immunosorbent assay were conducted to measure the expression of pro‐inflammatory cytokines. Protein expression levels were determined by western blotting. Immunofluorescence was used to detect nuclear translocation of nuclear factor kappa B (NF‐κB). We found that isorhamnetin at 20 and 40 μM reduced the proliferation of BEAS‐2B cells induced by TNF‐α. Isorhamnetin significantly decreased the expression of interleukin (IL)‐1β, IL‐6, IL‐8, and C‐X‐C motif chemokine ligand 10 in BEAS‐2B cells induced by TNF‐α. Additionally, 10 μM isorhamnetin effectively reduced cell migration induced by TNF‐α. Treatment with isorhamnetin inhibited the phosphorylation of mitogen‐activated protein kinase (MAPK) and NF‐κB pathways induced by TNF‐α. In summary, isorhamnetin inhibited the inflammation, proliferation, and migration of BEAS‐2B cells by regulating the MAPK and NF‐κB signaling pathways and is a drug candidate for asthma.

Potential mechanism of action of Jing Fang Bai Du San in the treatment of COVID-19 using docking and network pharmacology

“…As one of the major metabolites of quercetin, isorhamnetin has anti-inflammatory activity and reduces the expression of inflammatory genes in macrophages stimulated by LPS 33 . It also significantly inhibits the inflammation, proliferation and migration of BEAS-2B cells by regulating the MAPK and NF-κB signaling pathways 34 . Therefore, it is reasonable to speculate that acacetin, wogonin, and isorhamnetin are the main ingredients of JFBDS that treat COVID-19 by regulating PI3K/AKT, STAT3, MAPK, and NF-κB signaling pathways.…”

An Investigation Into the Mechanism of Li Chong Pill for Treating Endometriosis Based on Network Pharmacology and Molecular Docking Verification

“…43 Isorhamnetin, too, has distinct anti-in ammatory properties, 44 it can inhibit the in ammation, proliferation, and migration of BEAS-2B cells by regulating the MAPK and NF-κB signaling pathways and is a drug candidate for asthma. 45 According to the above analysis. Active compounds of LCP may play a major role in the treatment of EM with the function of anti-in ammatory, anti-oxidative stress, antiangiogenesis, smoothing of pain, and promoting ectopic tissue atrophy.…”

Therapeutic Effects and Molecular Mechanisms of Bioactive Compounds Against Respiratory Diseases: Traditional Chinese Medicine Theory and High-Frequency Use

“…Lindl., and Lepidium apetalum Willd. can reduce the production of inflammatory cytokines and oxidative stress to prevent LPS-induced ALI in mice through the NF-κB pathway (Cornélio Favarin et al, 2013;Huang et al, 2013;Lee et al, 2014b;Shu et al, 2014;Wang et al, 2014;Chi et al, 2016;Li et al, 2016;Luan et al, 2016;Liu et al, 2018a;Qian et al, 2019;Júlio de Souza et al, 2020;Ren et al, 2021). TLR4/MYD88, an upstream player of the NF-κB pathway, mediates the inflammation and ALI.…”