Isotachophoresis as a preseparation technique for liquid chromatography

Abstract

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High-performance liquid chromatography determination of phenolic components in wine using off-line isotachophoretic pretreatment

“…Concerning ITP and HPLC, these techniques are based on different physical properties which resulting in a powerful separation. In the literature, only limited numbers of papers have dealt with the combination of isotachophoresis with liquid chromatography [4][5][6][7][8][9].…”

Methodological aspects of an off‐line combination of preparative isotachophoresis and high‐performance liquid chromatography with mass spectrometry in the analysis of biological matrices

“…The main advantages of pITP using a micropreparative valve in the single or column‐coupling arrangement of the separation unit are described elsewhere . It was demonstrated that pITP in an off‐line mode could be used successfully as a sample pretreatment technique in the analysis of different multicomponent matrices prior to applying other high‐performance analytical techniques . The preconcentration capacity and sample clean‐up effect of pITP used for sample pretreatment in an off‐line mode using a micropreparative valve were previously noted in the literature, that is prior to HPLC analysis of herbicides in soil , urine matrices , flavonoids in plant extracts , and phenolic components in wine .…”

On-line isotachophoresis. A selective sample pretreatment prior to column liquid chromatographic analysis

“…Open tubular, free flow and gel packed systems can be distinguished. With open tubular systems the zones of interest can be removed from the separation compartment either by making a heart-cut [17][18][19][20], withdrawal using a microsyringe [21,22] or by applying continuous elution [23][24][25][26]. So far none of these methods has been used to couple ITP on-line with HPLC.…”