Isothermal Amplification of Nucleic Acids Coupled with Nanotechnology and Microfluidic Platforms for Detecting Antimicrobial Drug Resistance and Beyond

Alamolhoda, Seyedeh Zahra; Zarghami, Nosratollah; Kahroba, Houman; Mehdipour, Ahmad; Pourhassan‐Moghaddam, Mohammad; Jahanban‐Esfahlan, Rana; Milani, Morteza

doi:10.34172/apb.2022.004

Cited by 7 publications

(7 citation statements)

References 155 publications

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“…The methods used are as follows: GEAR, RPA, SDA, SMAP/SMAP2, HDA, NASBA, RCA, and TMA. [150,151] LAMP remains the most suitable technique with a more efficient direct amplification, more resistance to matrix interference, and more sensitivity. In addition, LAMP seems to be more adapted to a POC application [150,151].…”

Section: Other Amplification Methods For Amr Detectionmentioning

confidence: 99%

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The Revolution of Lateral Flow Assay in the Field of AMR Detection

et al. 2022

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The global spread of antimicrobial resistant (AMR) bacteria represents a considerable public health concern, yet their detection and identification of their resistance mechanisms remain challenging. Optimal diagnostic tests should provide rapid results at low cost to enable implementation in any microbiology laboratory. Lateral flow assays (LFA) meet these requirements and have become essential tools to combat AMR. This review presents the versatility of LFA developed for the AMR detection field, with particular attention to those directly triggering β-lactamases, their performances, and specific limitations. It considers how LFA can be modified by detecting not only the enzyme, but also its β-lactamase activity for a broader clinical sensitivity. Moreover, although LFA allow a short time-to-result, they are generally only implemented after fastidious and time-consuming techniques. We present a sample processing device that shortens and simplifies the handling of clinical samples before the use of LFA. Finally, the capacity of LFA to detect amplified genetic determinants of AMR by isothermal PCR will be discussed. LFA are inexpensive, rapid, and efficient tools that are easy to implement in the routine workflow of laboratories as new first-line tests against AMR with bacterial colonies, and in the near future directly with biological media.

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Section: Other Amplification Methods For Amr Detectionmentioning

confidence: 99%

“…The methods used are as follows: GEAR, RPA, SDA, SMAP/SMAP2, HDA, NASBA, RCA, and TMA. [ 150 , 151 ]…”

Section: Detection Of Amplicons By Lfiamentioning

confidence: 99%

The Revolution of Lateral Flow Assay in the Field of AMR Detection

et al. 2022

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“…As the RPA reaction is carried out at or near to the room temperature range, premixing of the sample with an initial reagent may proceed the reaction without compartmentalization. Despite its little applications in clinical sectors, authentications have been made regarding its use in the detection of viruses, bacteria, protozoa and human pathogens [ 56 , 57 ].…”

Section: Microfluidic Technology and Poctsmentioning

confidence: 99%

Prospects of Microfluidic Technology in Nucleic Acid Detection Approaches

et al. 2023

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Conventional diagnostic techniques are based on the utilization of analyte sampling, sensing and signaling on separate platforms for detection purposes, which must be integrated to a single step procedure in point of care (POC) testing devices. Due to the expeditious nature of microfluidic platforms, the trend has been shifted toward the implementation of these systems for the detection of analytes in biochemical, clinical and food technology. Microfluidic systems molded with substances such as polymers or glass offer the specific and sensitive detection of infectious and noninfectious diseases by providing innumerable benefits, including less cost, good biological affinity, strong capillary action and simple process of fabrication. In the case of nanosensors for nucleic acid detection, some challenges need to be addressed, such as cellular lysis, isolation and amplification of nucleic acid before its detection. To avoid the utilization of laborious steps for executing these processes, advances have been deployed in this perspective for on-chip sample preparation, amplification and detection by the introduction of an emerging field of modular microfluidics that has multiple advantages over integrated microfluidics. This review emphasizes the significance of microfluidic technology for the nucleic acid detection of infectious and non-infectious diseases. The implementation of isothermal amplification in conjunction with the lateral flow assay greatly increases the binding efficiency of nanoparticles and biomolecules and improves the limit of detection and sensitivity. Most importantly, the deployment of paper-based material made of cellulose reduces the overall cost. Microfluidic technology in nucleic acid testing has been discussed by explicating its applications in different fields. Next-generation diagnostic methods can be improved by using CRISPR/Cas technology in microfluidic systems. This review concludes with the comparison and future prospects of various microfluidic systems, detection methods and plasma separation techniques used in microfluidic devices.

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“…Inspite of its little applications in clinical sectors, authentications have been made regarding its use in detection of virus, bacteria, protozoa and human pathogens. [40,41] 2.5.7. Nucleic Acid Sequence based Amplification (NASBA)…”

Section: Multiple Displacement Amplification (Mda)mentioning

confidence: 99%

Prospects of Microfluidic Technology in Nucleic Acid Detection Approaches

Mumtaz¹,

Rashid²,

Ali³

et al. 2023

Preprint

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Conventional diagnostic techniques are based on utilization of analyte sampling, sensing and signalling on separate platforms for detection purposes, that must be evaded and integrated to a single step procedure in point of care (POC) testing devices. Therefore, the trend has been shifted towards utilization of microfluidic platforms for detection of analytes in biochemical, clinical and food technology due to its being expeditious. Microfluidic systems moulded with polymer substances or glass offer specific and sensitive detection of infectious and non-infectious diseases by providing innumerable benefits including less cost, good biological affinity, strong capillary action and simple process of fabrication. In the case of nucleic acid-based nanosensors, there are some challenges that need to be addressed such as cellular lysis, isolation and amplification of nucleic acid required to be accomplished prior to its detection. To avoid utilization of laborious steps for executing these steps, advances have been deployed in this perspective for on-chip sample preparation, amplification and detection which not only improves sensitivity and selectivity but also saves time and resources. This review emphasizes the significance of microfluidic technology for nucleic acid detection of infectious and non-infectious diseases. The implementation of isothermal amplification in concomitance with lateral flow assay greatly increases the binding efficiency of nanoparticles and biomolecules, improves limit of detection and sensitivity. Most importantly deployment of paper-based material made of cellulose reduces the overall cost. Microfluidic technology in nucleic acid testing has been discussed by explicating its applications in different fields. This review concludes with the prospects and proposes future directions in microfluidic based methods in disease diagnosis.

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Isothermal Amplification of Nucleic Acids Coupled with Nanotechnology and Microfluidic Platforms for Detecting Antimicrobial Drug Resistance and Beyond

Cited by 7 publications

References 155 publications

The Revolution of Lateral Flow Assay in the Field of AMR Detection

The Revolution of Lateral Flow Assay in the Field of AMR Detection

Prospects of Microfluidic Technology in Nucleic Acid Detection Approaches

Prospects of Microfluidic Technology in Nucleic Acid Detection Approaches

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