Isothiocyanate l−argininato copper(II) complexes – Solution structure, DNA interaction, anticancer and antimicrobial activity

Wojciechowska, Agnieszka; Bregier-Jarzebowska, R.; Komarnicka, Urszula K.; Kozieł, Sandra; Szuster‐Ciesielska, Agnieszka; Sztandera−Tymoczek, Monika; Jarząb, Anna; Staszak, Zbigniew; Witkowska, Danuta; Bojarska‐Junak, Agnieszka; Jezierska, Julia

doi:10.1016/j.cbi.2021.109636

Cited by 11 publications

(3 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Then, MTT assay was performed according to methods described elsewhere. 19 The 570 nm optical absorbance (A) was quantified utilizing a VICTOR X4 Multilabel Plate Reader (Perkin Elmer, Waltham, MA, USA). The obtained data were expressed as (i) a percentage of cell viability relative to the control and (ii) IC 50 (inhibitory concentration), representing the concentration that produced a 50% reduction in cytotoxic effect.…”

Section: Methodsmentioning

confidence: 99%

Potential Proallergenic Activity of Phytopathogenic Erysiphe palczewskii and Erysiphe convolvuli in in vitro Studies

Sztandera-Tymoczek,

Wdowiak-Wróbel,

Świderska

et al. 2023

JIR

View full text Add to dashboard Cite

Purpose Allergic diseases have reached epidemic proportions globally, affecting nearly 30% of the world’s population. One of the most prominent sources of allergens is fungi, causing up to 6% of respiratory diseases in the general population. However, the cause of respiratory allergies is not always identifiable. Therefore, we studied the ability of two representatives of common powdery mildew (Erysiphales), Erysiphe palczewskii and Erysiphe convolvuli , to induce a proinflammatory response in in vitro models of the upper and lower respiratory tract. Materials and Methods Two cell lines, BEAS-2B and A549, were used to mimic upper and lower respiratory epithelial cells. The toxicity of fungal extracts was assessed with MTT and flow cytometry assay. The production of reactive oxygen species in the cells was measured with flow cytometry. ELISA tests were used to determine the production of proinflammatory cytokines. The presence of the cell integrity marker was assessed with the immunofluorescence method. Results In both cell lines, the extract of E. palczewskii and E. convolvuli microfungi induced marked production of proinflammatory IL-1β, TNF-α, and GM-CSF cytokines involved in developing allergic reactions. The higher levels of these cytokines with higher reactive oxygen species synthesis positively correlated with the disruption of epithelial cell junctions. Conclusion We conclude that E. palczewskii and E. convolvuli microfungi have strong proinflammatory and proallergenic potential, but this finding needs in vivo confirmation.

show abstract

Section: Methodsmentioning

confidence: 99%

Potential Proallergenic Activity of Phytopathogenic Erysiphe palczewskii and Erysiphe convolvuli in in vitro Studies

Sztandera-Tymoczek,

Wdowiak-Wróbel,

Świderska

et al. 2023

JIR

View full text Add to dashboard Cite

show abstract

“…ROS have also been reported to directly induce other forms of deoxyribonucleic acid damage through oxidation of nucleoside bases (e.g., 8-oxo guanine can be formed) [121]. Most probably these processes are the main ones involved in the anticancer mode of action of these copper complexes [122][123][124].…”

Section: Cancer Treatmentmentioning

confidence: 99%

The Bright and Dark Sides of Reactive Oxygen Species Generated by Copper–Peptide Complexes

et al. 2022

Self Cite

View full text Add to dashboard Cite

Copper ions bind to biomolecules (e.g., peptides and proteins) playing an essential role in many biological and physiological pathways in the human body. The resulting complexes may contribute to the initiation of neurodegenerative diseases, cancer, and bacterial and viral diseases, or act as therapeutics. Some compounds can chemically damage biological macromolecules and initiate the development of pathogenic states. Conversely, a number of these compounds may have antibacterial, antiviral, and even anticancer properties. One of the most significant current discussions in Cu biochemistry relates to the mechanisms of the positive and negative actions of Cu ions based on the generation of reactive oxygen species, including radicals that can interact with DNA molecules. This review aims to analyze various peptide–copper complexes and the mechanism of their action.

show abstract

“…In light of the interesting coordination chemistry of L-arginine (Arg; Figure 1) and the diverse applications of the metal-Arg complexes [23,[53][54][55], the current work presents the synthesis of a new Zn(II) azido complex with Arg using a self-assembly technique. An analysis of the molecular and supramolecular structure of the new Zn(II) azido complex is presented.…”

Section: Introductionmentioning

confidence: 99%

A New Zn(II) Azido Complex of L-Arginine: X-ray Crystal Structure, Hirshfeld, and AIM Studies

Altowyan,

Yousri,

Albering

et al. 2023

Crystals

View full text Add to dashboard Cite

The synthesis and X-ray crystal structure analyses of the azido complex [Zn(N3)(Arg)2](N3)·3H2O, where Arg is L-arginine, were presented. The molecular structure of the complex was further studied using FT-IR spectra as well as atoms in molecules (AIM) theory. An analysis of the crystal data indicated monoclinic crystal system and P21 space group with a = 13.0283(5) Å, b = 15.2032(7) Å, c = 13.3633(6) Å, β = 114.3580(10)°, V = 2411.28(18) Å3, and Z = 4. Two of the [Zn(N3)(Arg)2](N3)·3H2O formulae represent the asymmetric unit of this complex where the geometric parameters of both units are slightly different. In [Zn(N3)(Arg)2](N3)·3H2O, the central Zn(II) ion is penta-coordinated with two Arg molecules as a bidentate ligand and one terminally coordinated azide ion. Each of the two Arg molecules are located trans to one another and coordinated with the Zn(II) via the N and O atoms of the amino and carboxylate groups, respectively. Hence, Zn(II) is five-coordinated and has a distorted square pyramidal coordination geometry. The supramolecular structure of the [Zn(N3)(Arg)2](N3)·3H2O complex was inspected using the Hirshfeld analysis. The O···H (26.6–28.4%), H···H (32.3–35.3%), and N···H (30.4–34.0%) contacts are the most significant interactions in the crystal structure of the [Zn(N3)(Arg)2](N3)·3H2O complex. The Zn–N, and Zn–O bonds have slight covalent interactions based on the AIM study.

show abstract

Isothiocyanate l−argininato copper(II) complexes – Solution structure, DNA interaction, anticancer and antimicrobial activity

Cited by 11 publications

References 64 publications

Potential Proallergenic Activity of Phytopathogenic Erysiphe palczewskii and Erysiphe convolvuli in in vitro Studies

Potential Proallergenic Activity of Phytopathogenic Erysiphe palczewskii and Erysiphe convolvuli in in vitro Studies

The Bright and Dark Sides of Reactive Oxygen Species Generated by Copper–Peptide Complexes

A New Zn(II) Azido Complex of L-Arginine: X-ray Crystal Structure, Hirshfeld, and AIM Studies

Contact Info

Product

Resources

About