Isotope Dilution Gas Chromatographic–Mass Spectrometric Method for the Determination of Isoflavonoids, Coumestrol, and Lignans in Food Samples

Mazur, Witold; Fotsis, Theodore; Wähälä, Kristina; Ojala, Sirpa; Salakka, Auli; Adlercreutz, Herman

doi:10.1006/abio.1996.0025

Cited by 302 publications

(237 citation statements)

References 9 publications

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“…Biochanin A and formononetin are provided in high quantities from the supplement; however, they are also supplied by the diet in small quantities in foods such as sunflower seeds, various beans and peanuts (Mazur et al, 1996(Mazur et al, , 1998. Not all subjects excreted the isoflavonoid metabolites and wider variation (20-40 fold) was seen between individuals: most subjects (96%) excreted O-dma but only 24% produce equol and of the lesser known metabolites, dihydrodaidzein was excreted by 64% and dihydrogenistein was detected in 12% of subjects.…”

Section: Discussionmentioning

confidence: 99%

Urinary isoflavonoid excretion is inversely associated with the ratio of protein to dietary fibre intake in young women

et al. 2004

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Objective: To examine the prevalence of excretion of urinary isoflavonoids in women and determine any relationships with accustomed macronutrient intake. Design: Volunteers in one of two 4-month studies. Study 1 was a randomised crossover study whereby subjects consumed a placebo or isoflavone supplement for 2 months and crossed over. Study 2 was a parallel design in which subjects consumed a placebo for 1 month and an isoflavone supplement for 3 months. Setting: All subjects were free-living, healthy volunteers. Subjects: A total of 25 (study 1, n ¼ 14; study 2, n ¼ 11) premenopausal women were recruited through advertisements. Interventions: Volunteers were supplemented for 2 months (study 1) or 3 months (study 2) with purified isoflavones (86 mg/ day) derived from red clover. Urinary isoflavonoids were measured during the placebo and the second month of isoflavone treatment. Macronutrient intakes were determined from weighed food records. Results: During isoflavone supplementation, the concentration of urinary total isoflavonoids increased by 15-fold (Po0.0001), with 5.4-fold variation between individuals. Multiple linear regression analysis showed that 24% of this variation could be explained by an interaction between dietary fibre and protein (P ¼ 0.047), with a highly significant inverse association between total isoflavonoid concentration and the protein to fibre ratio (r ¼ À0.51, P ¼ 0.009). Conclusions: Supplementation with purified isoflavones results in an increase in urinary isoflavonoid excretion and part of the individual variation in response is associated with an interaction between intakes of protein and dietary fibre. Whether manipulation of these macronutrients could enhance efficacy of isoflavone supplements remains to be determined.

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Section: Discussionmentioning

confidence: 99%

Urinary isoflavonoid excretion is inversely associated with the ratio of protein to dietary fibre intake in young women

et al. 2004

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“…Isoflavones and other phytoestrogens of coffee samples are usually determined with the use of HPLC [47] or gas chromatography-mass spectrometry (GC-MS) [48,49] or using automatic SPE and LC-MS/MS [50]. To free the aglicones of isoflavones from glycosides or glycoside esters and quantify them, acid hydrolysis [47] or enzymatic procedure [51] was applied.…”

Section: Isoflavones: Daidzein Genistein and Formononetinmentioning

confidence: 99%

Analytical methods applied for the characterization and the determination of bioactive compounds in coffee

Jeszka-Skowron

Zgoła‐Grześkowiak

Grześkowiak

2014

Eur Food Res Technol

125

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“…1 Soy protein (SP) is the major dietary source of isoflavones such as genistein, while flaxseed (FS) is one of the richest dietary sources of plant lignans, mainly secoisolariciresinol diglycoside (SDG). 2 When ingested, SDG is converted to mammalian lignans enterodiol and enterolactone by the intestinal microbiota. 3,4 The biological effects of lignans have been attributed particularly to enterolactone, which has been suggested to act as an endocrine active compound.…”

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confidence: 99%

“…2 Furthermore, whole soy beans have also been reported to contain minor amounts of plant lignans, e.g. secoisolariciresinol, but in concentrations approximately 1,000-fold lower when compared to that of FS.…”

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confidence: 99%

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Flaxseed attenuates the tumor growth stimulating effect of soy protein in ovariectomized athymic mice with MCF‐7 human breast cancer xenografts

Saarinen

Power

Chen

et al. 2006

Intl Journal of Cancer

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In several epidemiological studies, a phytoestrogen-rich diet containing lignans and isoflavones is associated with reduced breast cancer risk, but experimental findings are controversial. In postmenopausal mammary cancer xenograft model, flaxseed (FS), a rich source of plant lignans, reduced breast cancer growth, while soy protein (SP), a rich source of isoflavones, enhanced it. The intake of phytoestrogens is increasing particularly among postmenopausal women, emphasizing the importance of elucidating their interactive effects on breast cancer. Our study determined the effect of FS and SP diets, alone and in combination, on the established human breast cancer MCF-7 tumor growth in ovariectomized athymic nude mice. Tumor bearing mice were divided into 4 groups and fed for 25 weeks either the basal diet (BD), or BD supplemented with 10% FS, 20% SP or 10% FS and 20% SP. After estrogen deprivation, FS regressed the tumor size similar to that of control. SP initially regressed the tumors but starting at week 13, the tumors regressed significantly less than in control and 43% of the tumors were regrowing until the end of the experiment and were significantly larger in size than in control. The combination of SP with FS reduced the tumor growth similar to that of control, as suggested also by the reduced tumor cell proliferation index. In conclusion, dietary FS did not stimulate the growth of estrogen responsive MCF-7 cancers in ovariectomized mice, while long-term consumption of SP did. Furthermore, FS reduced the tumor growth stimulating effect of SP to the same level as control, suggesting tumor growth attenuating effect of FS. ' 2006 Wiley-Liss, Inc.

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Isotope Dilution Gas Chromatographic–Mass Spectrometric Method for the Determination of Isoflavonoids, Coumestrol, and Lignans in Food Samples

Cited by 302 publications

References 9 publications

Urinary isoflavonoid excretion is inversely associated with the ratio of protein to dietary fibre intake in young women

Urinary isoflavonoid excretion is inversely associated with the ratio of protein to dietary fibre intake in young women

Analytical methods applied for the characterization and the determination of bioactive compounds in coffee

Flaxseed attenuates the tumor growth stimulating effect of soy protein in ovariectomized athymic mice with MCF‐7 human breast cancer xenografts

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