Isotope Enhanced Approaches in Metabolomics

Gowda, G. A. Nagana; Shanaiah, Narasimhamurthy; Raftery, Daniel

doi:10.1007/978-94-007-4954-2_8

Cited by 15 publications

(8 citation statements)

References 104 publications

(103 reference statements)

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“…However, 13 C enrichment is not usually feasible in mammalian or human studies. To get around this problem, Shanaiah et al employed a chemical derivatization method or chemical tagging method to enrich unlabeled metabolites with 13 C [98]. In particular, by adding 13 C-acetic anhydride to urine or serum samples, it is possible to selectively 13 C-tag certain classes of metabolites (such as amino acids) through the 13 C acetylation of amines.…”

Section: 1h Nmr Spectroscopy For Metabolomicsmentioning

confidence: 99%

NMR Spectroscopy for Metabolomics Research

et al. 2019

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Over the past two decades, nuclear magnetic resonance (NMR) has emerged as one of the three principal analytical techniques used in metabolomics (the other two being gas chromatography coupled to mass spectrometry (GC-MS) and liquid chromatography coupled with single-stage mass spectrometry (LC-MS)). The relative ease of sample preparation, the ability to quantify metabolite levels, the high level of experimental reproducibility, and the inherently nondestructive nature of NMR spectroscopy have made it the preferred platform for long-term or large-scale clinical metabolomic studies. These advantages, however, are often outweighed by the fact that most other analytical techniques, including both LC-MS and GC-MS, are inherently more sensitive than NMR, with lower limits of detection typically being 10 to 100 times better. This review is intended to introduce readers to the field of NMR-based metabolomics and to highlight both the advantages and disadvantages of NMR spectroscopy for metabolomic studies. It will also explore some of the unique strengths of NMR-based metabolomics, particularly with regard to isotope selection/detection, mixture deconvolution via 2D spectroscopy, automation, and the ability to noninvasively analyze native tissue specimens. Finally, this review will highlight a number of emerging NMR techniques and technologies that are being used to strengthen its utility and overcome its inherent limitations in metabolomic applications.

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Section: 1h Nmr Spectroscopy For Metabolomicsmentioning

confidence: 99%

NMR Spectroscopy for Metabolomics Research

et al. 2019

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“…As life is built upon carbon chemistry, carbon-13 is a stable isotope tracer frequently used in NMR and MS metabolomics studies [ 69 , 70 , 71 ]. In NMR, individual 13C atoms can be tracked through isotopomers, i.e., isomers that differ only by the position of 13C, and followed over chemical transformations of metabolic pathways.…”

Section: Metabolic Phenotyping Of Cell Culture Metabolism: Analytimentioning

confidence: 99%

Metabolomic Approaches to Study Chemical Exposure-Related Metabolism Alterations in Mammalian Cell Cultures

Balcerczyk

Damblon

Elena-Herrmann

et al. 2020

IJMS

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Biological organisms are constantly exposed to an immense repertoire of molecules that cover environmental or food-derived molecules and drugs, triggering a continuous flow of stimuli-dependent adaptations. The diversity of these chemicals as well as their concentrations contribute to the multiplicity of induced effects, including activation, stimulation, or inhibition of physiological processes and toxicity. Metabolism, as the foremost phenotype and manifestation of life, has proven to be immensely sensitive and highly adaptive to chemical stimuli. Therefore, studying the effect of endo- or xenobiotics over cellular metabolism delivers valuable knowledge to apprehend potential cellular activity of individual molecules and evaluate their acute or chronic benefits and toxicity. The development of modern metabolomics technologies such as mass spectrometry or nuclear magnetic resonance spectroscopy now offers unprecedented solutions for the rapid and efficient determination of metabolic profiles of cells and more complex biological systems. Combined with the availability of well-established cell culture techniques, these analytical methods appear perfectly suited to determine the biological activity and estimate the positive and negative effects of chemicals in a variety of cell types and models, even at hardly detectable concentrations. Metabolic phenotypes can be estimated from studying intracellular metabolites at homeostasis in vivo, while in vitro cell cultures provide additional access to metabolites exchanged with growth media. This article discusses analytical solutions available for metabolic phenotyping of cell culture metabolism as well as the general metabolomics workflow suitable for testing the biological activity of molecular compounds. We emphasize how metabolic profiling of cell supernatants and intracellular extracts can deliver valuable and complementary insights for evaluating the effects of xenobiotics on cellular metabolism. We note that the concepts and methods discussed primarily for xenobiotics exposure are widely applicable to drug testing in general, including endobiotics that cover active metabolites, nutrients, peptides and proteins, cytokines, hormones, vitamins, etc.

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“…These metabolites are either indicative of various cellular processes, energy metabolism (e.g., ATP, GMP, NADPH), central carbon metabolism processes (e.g., sugar phosphates, phosphoenol-3-pyruvate), or structural and signaling functions (e.g., phospholipids). To increase the sensitivity and metabolome coverage of NMR, chemical derivatization with 13 C and 15 N isotopic tags has been described (Gowda et al 2012).…”

Section: Analytical Measurementmentioning

confidence: 99%

Metabolomic-Based Methods in Diagnosis and Monitoring Infection Progression

Fernández-García

Rojo

Rey-Stolle

et al. 2018

Experientia Supplementum

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A robust biomarker screening and validation is crucial for overcoming the current limits in the clinical management of infectious diseases. In this chapter, a general workflow for metabolomics is summarized. Subsequently, an overview of the major contributions of this omics science to the field of biomarkers of infectious diseases is discussed. Different approaches using a variety of analytical platforms can be distinguished to unveil the key metabolites for the diagnosis, prognosis, response to treatment and susceptibility for infectious diseases. To allow the implementation of such biomarkers into the clinics, the performance of large-scale studies employing solid validation criteria becomes essential. Focusing on the etiological agents and after an extensive review of the field, we present a comprehensive revision of the main metabolic biomarkers of viral, bacterial, fungal, and parasitic diseases. Finally, we discussed several articles which show the strongest validation criteria. Following these research avenues, precious clinical resources will be revealed, allowing for reduced misdiagnosis, more efficient therapies, and affordable costs, ultimately leading to a better patient management.

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Isotope Enhanced Approaches in Metabolomics

Cited by 15 publications

References 104 publications

NMR Spectroscopy for Metabolomics Research

NMR Spectroscopy for Metabolomics Research

Metabolomic Approaches to Study Chemical Exposure-Related Metabolism Alterations in Mammalian Cell Cultures

Metabolomic-Based Methods in Diagnosis and Monitoring Infection Progression

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