The chromatographic method of ion exchange resin in columns was used to study the isotopic enrichment of 10 B and H 3 10 BO 3. In two column chromatographic systems (S 1 : six acrylic columns 1800 mm length and 70 mm diameter; S 2 : six acrylic columns 1800 mm length and 30 mm diameter) was studied 10 B isotope separation in equilibrium involving aqueous boric acid and borate ions adsorbed on anionic resin of the quaternary ammonium type (Dowex 1X8) 100-200 "mesh". The production systems H 3 10 BO 3 were evaluated individually and in cascade process with 10 B transfer between both systems. The measurements of B in this study were evaluated by Inductively Coupled Plasma Mass Spectrometry and Thermal Ionization Mass Spectrometry. In the S 1 columns system displacement after 243 m (135 DBC) was possible obtain an medium enrichment in the last 20 cm, of 40 % atoms 10 B, corresponding to 2830 mg of H 3 10 BO 3. This mass was transferred to the S 2 column system which have introduced in the last 20 cm of the band medium enrichment of 47,8 atom% 10 B and this new band chromatography was displaced 21,6 m, thus obtaining the last centimeter band (1-0 cm) from enriched fraction 82 % atoms 10 B. Height Equivalent of Theoretical Plate (HETP) of stable isotopes of B (10 B and 11 B) was determined like being 1,0245 and 0,30 cm, respectively.