Isotopic analyses by ICP-MS in clinical samples

Rodushkin, Ilia; Engström, Emma; Baxter, Douglas C.

doi:10.1007/s00216-012-6457-x

Cited by 39 publications

(27 citation statements)

References 149 publications

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“…Many interesting applications of isotopic analysis in clinical samples were recently reviewed by Rodushkin et al, with a special focus on lead [81].…”

Section: Isotopic Analysismentioning

confidence: 99%

Current Role of ICP–MS in Clinical Toxicology and Forensic Toxicology: A Metallic Profile

et al. 2014

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As metal/metalloid exposure is inevitable owing to its omnipresence, it may exert toxicity in humans. Recent advances in metal/metalloid analysis have been made moving from flame atomic absorption spectrometry and electrothermal atomic absorption spectrometry to the multi-elemental inductively coupled plasma (ICP) techniques as ICP atomic emission spectrometry and ICP-MS. ICP-MS has now emerged as a major technique in inorganic analytical chemistry owing to its flexibility, high sensitivity and good reproducibility. This in depth review explores the ICP-MS metallic profile in human toxicology. It is now routinely used and of great importance, in clinical toxicology and forensic toxicology to explore biological matrices, specifically whole blood, plasma, urine, hair, nail, biopsy samples and tissues.

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“…Many interesting applications of isotopic analysis in clinical samples were recently reviewed by Rodushkin et al, with a special focus on lead [81].…”

Section: Isotopic Analysismentioning

confidence: 99%

Current Role of ICP–MS in Clinical Toxicology and Forensic Toxicology: A Metallic Profile

et al. 2014

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“…Inductively coupled plasma mass spectrometry (ICP-MS) is the most versatile technique for determination of trace elements [22, 23]. Ion chromatography (IC) is an intensively developing technique for simultaneous determination of ions in samples of complex matrices [24].…”

Section: Introductionmentioning

confidence: 99%

Alterations of Hair and Nail Content of Selected Trace Elements in Nonoccupationally Exposed Patients with Chronic Depression from Different Geographical Regions

Błażewicz

Liao²,

Liao³

et al. 2017

BioMed Research International

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The aim of this study was to determine if altered levels of selected trace elements manifest themselves during chronic depression. To identify elements strongly associated with chronic depression, relationships between the elemental contents of hair and nails and the interelement correlations were checked. Inductively coupled plasma mass spectrometry and ion chromatography were used to evaluate the contents of Zn, Cu, Co, Pb, Mn, and Fe in hair and nail samples from a total of 415 subjects (295 patients and 120 healthy volunteers). The study included logistic regression models to predict the probability of chronic depression. To investigate possible intercorrelations among the studied elements, the scaled principal component analysis was used. The research has revealed differences in TE levels in the group of depressed men and women in comparison to the healthy subjects. Statistically significant differences in both hair and nails contents of several elements were observed. Our study also provides strong evidence that the intermediary metabolism of certain elements is age- and gender-dependent. Zn, Mn, Pb, and Fe contents in hair/nails seem to be strongly associated with chronic depression. We found no statistically significant residence-related differences in the contents of studied elements in nonoccupationally exposed patients and healthy subjects.

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“…These included inductively coupled plasma-mass spectrometry (ICP-MS), which involves spraying a solution containing iron into argon heated by electromagnetic fields to around 6,000°C (inductively coupled plasma) that generates ions (that are then introduced to a quadrupole mass spectrometer) whose frequency is subsequently measured by an electron multiplier (Perkin-Elmer 2011). This technique is widely used to measure iron in biological samples (Li et al 2012;Rodushkin et al 2013) but in this setting, ICP-MS lacked the sensitivity to detect intracellular iron levels in AGS cells when cell numbers were low. In contrast, when cell numbers were increased (and total volume decreased), the assay proved to be extremely precise.…”

Section: Discussionmentioning

confidence: 99%

Measurement of total iron in Helicobacter pylori-infected gastric epithelial cells

2014

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Despite the evidence suggesting a role for Helicobacter pylori in the induction of systemic iron deficiency anaemia, little is known about the possibility of infection-associated changes in cellular iron homeostasis at the gastric epithelium. In this study we compared four different techniques for measuring iron in AGS cells, a gastric epithelial cell line that is widely used to model to H. pylori infection in vitro. Inductively coupled plasma-mass spectrometry proved to be an efficient method, but only when large numbers of cells were used. Two colorimetric assays that included the use of concentrated hydrochloric acid with or without potassium ferrocyanide detected iron in the micromolar but not the nanomolar range in cell-free standards. However, the third colorimetric assay that incorporated ferrozine proved to be highly accurate at detecting iron in the nanomolar range, and was able to detect iron in AGS cells, Moreover, using this assay, we were able to show that the level of iron in H. pylori-infected AGS cells is significantly increased when compared to uninfected cells.

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Isotopic analyses by ICP-MS in clinical samples

Cited by 39 publications

References 149 publications

Current Role of ICP–MS in Clinical Toxicology and Forensic Toxicology: A Metallic Profile

Current Role of ICP–MS in Clinical Toxicology and Forensic Toxicology: A Metallic Profile

Alterations of Hair and Nail Content of Selected Trace Elements in Nonoccupationally Exposed Patients with Chronic Depression from Different Geographical Regions

Measurement of total iron in Helicobacter pylori-infected gastric epithelial cells

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