Isotopic fingerprints indicate distinct strategies of Fe uptake in rice

Liu, Chengshuai; Gao, Ting; Liu, Yuhui; Liu, Jingyu; Li, Fangbai; Chen, Zhenwu; Li, Yongzhu; Lv, Yiwen; Song, Zhiyi; Reinfelder, John R.; Huang, Weilin

doi:10.1016/j.chemgeo.2019.07.002

Cited by 16 publications

(34 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Iron isotope fractionation due to the uptake through complexation of Fe(III) would then depend on the variation of isotope compositions between different Fe(III) species in the rhizosphere and in the root epidermis. This phenomenon of varied isotope fractionation effects was observed for rice (Liu et al, 2019), which can utilize both Fe(III) and Fe(II) from the rhizosphere (Ishimaru et al, 2006). At our site, the difference between the Fe isotope compositions of the whole plant and the Fe HCl pool (Δ 56 Fe WholePlant‐HCl_Topsoil ) was only marginal.…”

Section: Discussionmentioning

confidence: 80%

“…Kiczka et al (2010) and Guelke-Stelling and von Blanckenburg (2012) argued that the Fe isotopic signature of a plant did not only depend on the Fe uptake strategy, but also on the nutrient availability in the growth substrate. This statement was only recently confirmed by a study where rice plants showed different Fe isotope compositions when growing under Fe-sufficient or Fe-deficient conditions (Liu et al, 2019). However, evidence of varying Fe isotope compositions upon different Fe availabilities is still needed to better understand Fe isotope fractionation in other graminaceous plants.…”

Section: Introductionmentioning

confidence: 97%

See 1 more Smart Citation

Iron isotope fractionation in soil and graminaceous crops after 100 years of liming in the long‐term agricultural experimental site at Berlin‐Dahlem, Germany

Wang

Berns

et al. 2020

European J Soil Science

View full text Add to dashboard Cite

Sustainable arable cropping relies on repeated liming. Yet, the associated increase in soil pH can reduce the availability of iron (Fe) to plants. We hypothesized that repeated liming, but not pedogenic processes such as lessivage (i.e., translocation of clay particles), alters the Fe cycle in Luvisol soil, thereby affecting Fe isotope composition in soils and crops. Hence, we analysed Fe concentrations and isotope compositions in soil profiles and winter rye from the long‐term agricultural experimental site in Berlin‐Dahlem, Germany, where a controlled liming trial with three field replicates per treatment has been conducted on Albic Luvisols since 1923. Heterogeneity in subsoil was observed at this site for Fe concentration but not for Fe isotope composition. Lessivage had not affected Fe isotope composition in the soil profiles. The results also showed that almost 100 years of liming lowered the concentration of the HCl‐extractable Fe that was potentially available for plant uptake in the surface soil (0–15 cm) from 1.03 (standard error (SE) 0.03) to 0.94 (SE 0.01) g kg−1. This HCl‐extractable Fe pool contained isotopically lighter Fe (δ56Fe = −0.05 to −0.29‰) than the bulk soil (δ56Fe = −0.08 to 0.08‰). However, its Fe isotope composition was not altered by the long‐term lime application. Liming resulted in relatively lower Fe concentrations in the roots of winter rye. In addition, liming led to a heavier Fe isotope composition of the whole plants compared with those grown in the non‐limed plots (δ56FeWholePlant_ + Lime = −0.12‰, SE 0.03 vs. δ56FeWholePlant_‐Lime = −0.21‰, SE 0.01). This suggests that the elevated soil pH (increased by one unit due to liming) promoted the Fe uptake strategy through complexation of Fe(III) from the rhizosphere, which favoured heavier Fe isotopes. Overall, the present study showed that liming and a related increase in pH did not affect the Fe isotope compositions of the soil, but may influence the Fe isotope composition of plants grown in the soil if they alter their Fe uptake strategy upon the change of Fe availability. Highlights Fe concentrations and stocks, but not Fe isotope compositions, were more heterogeneous in subsoil than in topsoil. Translocation of clay minerals did not result in Fe isotope fractionation in the soil profile of a Luvisol. Liming decreased Fe availability in topsoil, but did not affect its δ56Fe values. Uptake of heavier Fe isotopes by graminaceous crops was more pronounced at elevated pH.

show abstract

Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 97%

Iron isotope fractionation in soil and graminaceous crops after 100 years of liming in the long‐term agricultural experimental site at Berlin‐Dahlem, Germany

Wang

Berns

et al. 2020

European J Soil Science

View full text Add to dashboard Cite

show abstract

“…When grown in waterlogged soil, no visible phenotypic differences were observed between the fit mutants and wild‐type plants (Figure 3B, C). Rice mainly uses strategy II to obtain Fe in aerobic soil lacking soluble Fe(II) (Liu et al 2019). We speculated that the strategy II components were damaged in the fit mutants.…”

Section: Resultsmentioning

confidence: 99%

“…Ishimaru et al (2006) confirmed that rice takes up both Fe(III)‐phytosiderophore and Fe(II). The results of a recent study suggest that rice uses strategy II to absorb Fe under Fe‐deficient conditions, whereas strategy I is applied under Fe‐sufficient conditions (Liu et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Oryza sativa FER‐LIKE FE DEFICIENCY‐INDUCED TRANSCRIPTION FACTOR (OsFIT/OsbHLH156) interacts with OsIRO2 to regulate iron homeostasis

Liang

Zhang

Yang

et al. 2020

JIPB

View full text Add to dashboard Cite

Iron (Fe) is indispensable for the growth and development of plants. It is well known that FER-LIKE FE DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT) is a key regulator of Fe uptake in Arabidopsis. Here, we identify the Oryza sativa FIT (also known as OsbHLH156) as the interacting partner of IRON-RELATED BHLH TRAN-SCRIPTION FACTOR 2 (OsIRO2) that is critical for regulating Fe uptake. The OsIRO2 protein is localized in the cytoplasm and nucleus, but OsFIT facilitates the accumulation of OsIRO2 in the nucleus. Loss-of-function mutations of OsFIT result in decreased Fe accumulation, severe Fe-deficiency symptoms, and disrupted expression of Fe-uptake genes. In contrast, OsFIT overexpression promotes Fe accumulation and the expression of Fe-uptake genes. Genetic analyses indicate that OsFIT and OsIRO2 function in the same genetic node. Further analyses suggest that OsFIT and OsIRO2 form a functional transcription activation complex to initiate the expression of Fe-uptake genes. Our findings provide a mechanism understanding of how rice maintains Fe homeostasis.

show abstract

“…Thus, in contrast to other cereal species [44], rice partially adopts Strategy-I Fe uptake, in which Fe(II) ions are directly taken up by IRON-REGULATED TRANSPORTERs (IRTs) [41,45]. Although a functional Strategy-II uptake system is present in rice, when Fe(II) ions are abundant in the rhizosphere, Fe acquisition is mainly achieved through Strategy-I uptake, and genes involved in Strategy-II Fe uptake are down-regulated [43,46].…”

Section: Fe Uptake System and Phytosiderophore Synthesismentioning

confidence: 99%

Potential Implications of Interactions between Fe and S on Cereal Fe Biofortification

Kawakami

Bhullar

2020

IJMS

View full text Add to dashboard Cite

Iron (Fe) and sulfur (S) are two essential elements for plants, whose interrelation is indispensable for numerous physiological processes. In particular, Fe homeostasis in cereal species is profoundly connected to S nutrition because phytosiderophores, which are the metal chelators required for Fe uptake and translocation in cereals, are derived from a S-containing amino acid, methionine. To date, various biotechnological cereal Fe biofortification strategies involving modulation of genes underlying Fe homeostasis have been reported. Meanwhile, the resultant Fe-biofortified crops have been minimally characterized from the perspective of interaction between Fe and S, in spite of the significance of the crosstalk between the two elements in cereals. Here, we intend to highlight the relevance of Fe and S interrelation in cereal Fe homeostasis and illustrate the potential implications it has to offer for future cereal Fe biofortification studies.

show abstract

Isotopic fingerprints indicate distinct strategies of Fe uptake in rice

Cited by 16 publications

References 31 publications

Iron isotope fractionation in soil and graminaceous crops after 100 years of liming in the long‐term agricultural experimental site at Berlin‐Dahlem, Germany

Iron isotope fractionation in soil and graminaceous crops after 100 years of liming in the long‐term agricultural experimental site at Berlin‐Dahlem, Germany

Oryza sativa FER‐LIKE FE DEFICIENCY‐INDUCED TRANSCRIPTION FACTOR (OsFIT/OsbHLH156) interacts with OsIRO2 to regulate iron homeostasis

Potential Implications of Interactions between Fe and S on Cereal Fe Biofortification

Contact Info

Product

Resources

About