Isotopomer signatures of soil-emitted N2O under different moisture conditions—A microcosm study with arable loess soil

Well, Reinhard; Kurganova, I. N.; Gerenyu, Valentin Lopes de; Flessa, Heinz

doi:10.1016/j.soilbio.2006.05.003

Cited by 104 publications

(106 citation statements)

References 44 publications

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“…[36] The use of SP is a powerful tool for differentiating microbial sources of N 2 O in soil ecosystems [Bol et al, 2003;Sutka et al, 2006Sutka et al, , 2008Well et al, 2006]. SP is unaffected by O exchange with substrates, water and atmospheric O 2 , and provides a conservative tracer of production [Sutka et al, 2006;Toyoda et al, 2005].…”

Section: Discussionmentioning

confidence: 99%

Evidence for the predominance of denitrification as a source of N₂O in temperate agricultural soils based on isotopologue measurements

Opdyke

Ostrom

2009

Global Biogeochemical Cycles

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Section: Discussionmentioning

confidence: 99%

Evidence for the predominance of denitrification as a source of N₂O in temperate agricultural soils based on isotopologue measurements

Opdyke

Ostrom

2009

Global Biogeochemical Cycles

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“…The headspace was later flushed with a fresh air flow of 20 mL/min. Sampling of the exhaust air for N 2 O, CH 4 and CO 2 analyzes was done with glass vials (20 mL) for 37 days (Well et al 2006). Sampling was done daily at the same time.…”

Section: Methodsmentioning

confidence: 99%

Soil, slurry and application effects on greenhouse gas emissions

Severin¹,

Fuß²,

Well³

et al. 2015

Plant Soil Environ.

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In conjunction with soil type and climate, the use of nitrogen fertilizers is a major factor affecting nitrous oxide emissions. This study compares injection of pig slurry and pig slurry digestate at 15 or 20 cm depths with trailinghose application followed by immediate incorporation. The work was based on a laboratory microcosm experiment with undisturbed soil cylinders (0-30 cm depth) from three arable soils (Histosol, Gleysol and Plaggic Anthrosol). Soil cylinders were fertilized with pig slurry and pig slurry digestate (150 kg N/ha) and emissions of N 2 O, CH 4 and CO 2 were monitored. The comparison of application techniques over a 37-day period show that soil type and application technique, had a strong (P < 0.001) impact on N 2 O emissions. Fertilization with pig slurry showed no significantly higher N 2 O emissions than pig slurry digestate. Fertilizer injection significantly increased N 2 O emissions compared to fertilization with a trailing-hose with incorporation.

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“…More specifically, the location at which an isotope substitution occurs in a product can be used to track which substrate has been consumed and how the product was synthesized during a biochemical reaction. Isotopomer detection has been applied in only a few experimental observations due to the rather complicated techniques involved [e.g., Toyoda et al, 2005;Well et al, 2006]. However, to our knowledge, there is no general approach aimed at modeling kinetic isotopic effects in enzyme-catalyzed reactions in which isotopomer substrates and products intervene simultaneously.…”

Section: Introductionmentioning

confidence: 99%

Mathematical treatment of isotopologue and isotopomer speciation and fractionation in biochemical kinetics

Maggi

Riley

2010

Geochimica et Cosmochimica Acta

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Abstract.We present a mathematical treatment of the kinetic equations that describe isotopologue and isotopomer speciation and fractionation during enzymecatalyzed biochemical reactions. These equations, presented here with the name GEBIK (General Equations for Biochemical Isotope Kinetics) and GEBIF (General Equations for Biochemical Isotope Fractionation), take into account microbial biomass and enzyme dynamics, reaction stoichiometry, isotope substitution number, and isotope location within each isotopologue and isotopomer.In addition to solving the complete GEBIK and GEBIF, we also present and discuss two approximations to the full solutions under the assumption of biomassfree and enzyme steady-state, and under the quasi-steady-state assumption as applied to the complexation rate. The complete and approximate approaches are applied to observations of biological denitrification in soils. Our analysis highlights that the full GEBIK and GEBIF provide a more accurate description of concentrations and isotopic compositions of substrates and products throughout the reaction than do the approximate forms. We demonstrate that the isotopic effects of a biochemical reaction depend, in the most general case, on substrate and complex concentrations and, therefore, the fractionation factor is a function of time. We also demonstrate that inverse isotopic effects can occur for values of the fractionation factor smaller than 1, and that reactions that do not discriminate isotopes do not necessarily imply a fractionation factor equal to 1.

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Isotopomer signatures of soil-emitted N2O under different moisture conditions—A microcosm study with arable loess soil

Cited by 104 publications

References 44 publications

Evidence for the predominance of denitrification as a source of N₂O in temperate agricultural soils based on isotopologue measurements

Evidence for the predominance of denitrification as a source of N₂O in temperate agricultural soils based on isotopologue measurements

Soil, slurry and application effects on greenhouse gas emissions

Mathematical treatment of isotopologue and isotopomer speciation and fractionation in biochemical kinetics

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Isotopomer signatures of soil-emitted N2O under different moisture conditions—A microcosm study with arable loess soil

Cited by 104 publications

References 44 publications

Evidence for the predominance of denitrification as a source of N2O in temperate agricultural soils based on isotopologue measurements

Evidence for the predominance of denitrification as a source of N2O in temperate agricultural soils based on isotopologue measurements

Soil, slurry and application effects on greenhouse gas emissions

Mathematical treatment of isotopologue and isotopomer speciation and fractionation in biochemical kinetics

Contact Info

Product

Resources

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Evidence for the predominance of denitrification as a source of N₂O in temperate agricultural soils based on isotopologue measurements

Evidence for the predominance of denitrification as a source of N₂O in temperate agricultural soils based on isotopologue measurements