Isotype analysis of gerbil–mouse heterohybridomas by RT-PCR

Tyrosinase (<i>Tyr</i>) Gene Mutation in Albino Mongolian Gerbil (<i>Meriones unguiculatus</i>)

“…However, the mechanism of these sensitivities remains to be identified. So far, we have reported molecular information on immunoglobulin [28] [29] and variation in the IgG subclass [30] in the agouti (MON/Num/a) strain to investigate acquired immunity in gerbil, and have established methods for producing gerbil mAb [31] and cytokines [32] with heterohybridoma techniques. Although immune systems have not been compared in detail between albino (MON/Num/c) and agouti strains yet, use of the albino strain for immunological research may further contribute to the establishment of new strains as models of infectious disease.…”

Conditioned medium from gerbil—mouse T cell heterohybridomas improved antibody secretion

“…Three gerbil–mouse heterohybridomas—B11D2(C2)(which secrete gerbil IgG1 specific to keyhole limpet hemocyanin; KLH) [ 21 , 22 ], D9(E4), and D9(E6)C2B3, which were generated by fusing gerbil splenocytes with mouse myeloma cells (P3-X63-Ag8.653, provided by the RIKEN BioResource Center)—and mouse myeloma cells (P3-X63-Ag8.653) were cultured in RPMI-1640 medium (Wako, Osaka, Japan) supplemented with 10% heat-inactivated fetal bovine serum (JRH Biosciences, Tokyo, Japan), 100 U/ml penicillin (Meiji, Tokyo, Japan), 100 µ g/ml streptomycin (Meiji), MEM nonessential amino acids (Invitrogen Gibco, Tokyo, Japan), 5 × 10 −2 M 2-mercaptoethanol (Wako, Tokyo, Japan), and 2 µ g/ml NaHCO 3 (Nacalai Tesque, Tokyo, Japan). Cells were maintained in a humidified incubator at 37°C with 5% CO 2 .…”

“…In case the products from those cells are gerbil cytokines, they would be useful reagents for the culture of gerbil cells. In this study, to characterize the two lines, D9(E4) and D9(E6)C2B3, which did not show Igs secretion, we evaluated their effect on the cell proliferation of and antibody secretion by another heterohybridoma that secretes gerbil IgG1 [ 21 ]. This report describes the production of stable T cell heterohybridomas and the characteristics of the cells secreting a factor capable of stimulating the production of Ig.…”

Use of Genomic <i>In Situ</i> Hybridization to Identify Chromosomes in Gerbil-Mouse Heterohybridomas

“…Gerbil-mouse heterohybridoma lines b11D2(c2), b11E2(D5).M, b5-3, and D5 continuously secreting gerbil mab (which secrete gerbil igG1, igM, igG2, and igG2 [11] and have median chromosome numbers of 70, 68, 87, and 90 [10], respectively) and mouse myeloma cells having a median chromosome number of 59 [10] (P3-X63-ag8.653, provided by the RikEN bioResource center) were cultured in RPMi-1640 medium (Wako, osaka, japan) supplemented with 10% heat-inactivated fetal bovine serum (jRH biosciences, Tokyo, japan), 100 U/ml penicillin (Meiji, Tokyo, japan), 100 µg/ml streptomycin (Meiji), MEM nonessential amino acids (Gibco, Life Technologies, Tokyo, japan), 5 × 10 −2 M 2-mercaptoethanol (Wako, Tokyo, japan), and 2 µg/ml NaHco 3 (Nacalai Tesque, Tokyo, japan). cells were maintained in a humidified incubator at 37°C under 5% co 2 .…”