Isozyme and Dissociated Protein Profiles of Normal Plants and Somaclonal Variants of Banana Obtained by in Vitro Culture

Carvalho, Marília; Derbyshire, Eric; Martins-Corder, Maisa Pimentel; Mendes, Beatriz Madalena Januzzi; Neto, Aurélio Rúbio

doi:10.17660/actahortic.1998.490.45

Cited by 5 publications

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“…On the other hand POD pattern of the V133 was most polymorphic. The dissociation of the POD and EST isoforms and induction of new forms was related to somaclonal variation in in vitro plants in other species as well (9)(10)(11). All isoform of the acid phosphatase were very intensive unlike previous report previously (7) and highly polymorphic regardless of the clone origin.…”

Section: Discussionmentioning

confidence: 66%

Isoenzyme markers for assessment of in vitro plants from Ruscus Aculeatus L.

Ivanova¹,

Angelov²,

Bosseva³

et al. 2015

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PURPOSE: Ruscus aculeatus L. is an economically important ornamental and medicinal plant gathered mainly from the wild. Micropropagation protocols have been suggested for rapid production of clonal material and ex situ conservation of Ruscus species to avoid further over-exploitation of its natural resources. Variances in propagation rate and plant habitus were reported previously as important traits of the specific clones. Izoenzyme markers are proposed for assessment of the possible somaclonal variability induced during the in vitro propagation and the efficiency of the micropropagation procedure. METHODS: The isoenzyme profiles of three enzyme systems (peroxidases, esterases and acid phosphatase) of seven in vitro clones and native control were compared using discontinuous PAGE system. RESULTS: Induction of additional isoforms was frequent in the in vitro plants and clonespecific variations were detected. Acid phosphatase profiles were most polymorphic compared to less variable esterase and peroxidase ones. Isoenzyme profiles were not particularly correlated with the geographical origin of the accessions. CONCLUSIONS: The less polymorphic peroxidase and esterase systems could be used as markers for somaclonal changes by in vitro cultivation of R. aculeatus and selection of prospective clones.

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Section: Discussionmentioning

confidence: 66%

Isoenzyme markers for assessment of in vitro plants from Ruscus Aculeatus L.

Ivanova¹,

Angelov²,

Bosseva³

et al. 2015

TJS

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“…Karyological analysis, for example, cannot reveal alterations in specific genes or small chromosomal rearrangements (Isabel et al, 1993). Biochemical markers like, isozyme markers provide a convenient method for detecting genetic changes (Carvalho et al, 1998), but are subject to ontogenic variations. Their use is also limited and only DNA regions coding for soluble proteins can be sampled.…”

Section: Issn: 2319-7706 Volume 7 Number 07 (2018)mentioning

confidence: 99%

Molecular Analysis of Micropropagated Banana Variants (Musa spp. ‘AAA’) Using RAPD Markers

Shiddalingeswara¹,

Prakash²,

Kustagi³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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“…About 80% of the somaclonal variations are dwarf-type variants [8], which are almost invariably linked to low productivity. Thus, many studies have been realized to detect dwarf variants at early stages of development, using gibberellic acid [9,10], isozyme profile polymorphism [11] and molecular markers [12,13]. The first technique is easily applicable to routine micropropagation, although it detects only gibberellin-sensitive variants.…”

Section: Introductionmentioning

confidence: 99%

Stimulation of bananain vitroshoot growth by yellow-cellophane-film shading

Matsumoto

Caldas

2007

Fruits

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Stimulation of banana in vitro shoot growth by yellow-cellophane-film shading. Abstract-Introduction. Many shoots or bud-like structures are lost in the final stage of plant micropropagation because of being too short for handling. Yellow and red lights induce in vitro plant growth of some species, and blue light reduces it instead. Yellow cellophane film passes through from the yellow to red light spectrum but cuts off the blue one. Growth of banana in vitro shoots was thus compared with or without yellow-cellophane-film shading. Materials and methods. Field-grown banana normal and dwarf variant plants of Nanicão cv. (Musa sp., AAA group, Cavendish subgroup) derived from micropropagation were re-established in vitro. They were then transferred to MS medium without growth regulator in test tubes covered by yellow cellophane film under cool white fluorescent lamps at a light intensity of 46 μmol•m-2 •s-1. Shoot growth was measured as height was evaluated after (20 and 30) days of culture. Results and discussion. The yellow-cellophane-film shading stimulated the growth of banana in vitro shoots. When cultured under the yellow-cellophane-film shading for 20 days, the normal trueto-type plants showed an increase of about 25% in shoot height compared with the plants cultured without cellophane film. Similar growth response was also observed on the in vitro shoots of the dwarf plants but the increment was only about 12%. This shading technique can aid shoot elongation and consequently reduction of plantlet loss at the final stage of plant micropropagation. The differing sensibility of the normal true-to-type plants and dwarf variants to the yellowcellophane-film shading may be used for reduction of the dwarf variant population in banana micropropagation. Conclusion. Yellow-cellophane-film shading stimulates growth of banana in vitro shoots. Brazil / Musa / plant propagation / micropropagation / light requirements / radiosensitivity Stimulation de la croissance in vitro de plantules de bananier sous film de cellophane jaune. Résumé-Introduction. De nombreuses plantules ou autres structures analogues à des bourgeons sont perdues lors de la dernière phase de micropropagation des plants, car elles sont trop courtes pour être manipulées. Les lumières jaune et rouge induisent la croissance in vitro de certaines espèces, alors que la lumière bleue la réduit. Le film de cellophane jaune permet le passage d'un spectre de lumière du jaune au rouge mais arrête le bleu. La croissance in vitro des plantules de banane a alors été comparée avec ou sans film de cellophane jaune. Matériel et méthodes. Des plants normaux de bananiers et des variants nains du cv. Nanicão (Musa sp., groupe AAA, sous-groupe Cavendish), issus de micropropagation et cultivés en champ, ont été remis en culture in vitro. Les vitroplants ont été alors transférés, sur milieu MS sans régulateur de croissance, dans des tubes à essai obturés par un film de cellophane jaune, sous des lampes fluorescentes à lumière froide blanche présentant une intensité de 46 μmol•m...

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Isozyme and Dissociated Protein Profiles of Normal Plants and Somaclonal Variants of Banana Obtained by in Vitro Culture

Cited by 5 publications

References 0 publications

Isoenzyme markers for assessment of in vitro plants from Ruscus Aculeatus L.

Isoenzyme markers for assessment of in vitro plants from Ruscus Aculeatus L.

Molecular Analysis of Micropropagated Banana Variants (Musa spp. ‘AAA’) Using RAPD Markers

Stimulation of bananain vitroshoot growth by yellow-cellophane-film shading

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