Isozyme Evidence of Reticulate Evolution in Mosses: Plagiomnium medium is an Allopolyploid of P. ellipticum x P. insigne

Wyatt, Robert; Odrzykoski, Ireneusz J.; Stoneburner, Ann

doi:10.2307/2419725

Cited by 60 publications

(44 citation statements)

References 22 publications

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“…However, there is now a growing body of evidence, mainly from the use of isozyme markers, for allopolyploidization. Thus isozyme analyses suggest that several mosses including Weissia exserta, Plagiomnium medium (Wyatt, Odrzykoski & Stoneburner, 1992), Sphagnum russowii (Cronberg, 1996), Polytrichastrum pallidisetum, P. ohionense, and P. sexangulare (Derda & Wyatt, 2000) and a few liverworts, e.g. Porella baueri (Boisselier-Dubayle, Lambourdiere & Bischler, 1997) and Corsinia coriandrina (Boisselier-Dubayle & Bishler, 1998) are allopolyploids.…”

Section: Introductionmentioning

confidence: 99%

Molecular cytogenetic analyses of haploid and allopolyploidPelliaspecies

Orzechowska

Siwinska

Małuszyńska

2010

Journal of Bryology

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The allopolyploid origin of several bryophyte species was reported approximately 20 years ago. Among them the best characterized is Pellia borealis, a hybrid of two cryptic sibling species: Pellia epiphylla-species N and P. epiphylla-species S. Genomes of the allopolyploid liverwort P. borealis and its progenitors were investigated using conventional as well as molecular cytogenetic techniques. The nuclear DNA content and cell cycle phase in the thallus nuclei was established using flow cytometry. Fluorescent differential staining, C-banding and fluorescent in situ hybridization (FISH) with 26S and 5S ribosomal DNA (rDNA) probes revealed new features of the chromosomes in the P. epiphylla-P. borealis complex. Some characteristics found in both the polyploid and haploid karyotypes support earlier suggestions about the allopolyploid origin of P. borealis. The banding pattern observed on P. borealis chromosomes suggests the occurrence of structural changes in the allopolyploid genome. The number and localization of rDNA sequences were established and simultaneous FISH with 26S and 5S rDNA probes showed colocalization of both types of ribosomal RNA (rRNA) genes in Pellia chromosomes. A nuclear DNA estimate showed that the P. borealis nuclear DNA content is about twice that of the largest known nuclear genome previously known in bryophytes.

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Section: Introductionmentioning

confidence: 99%

Molecular cytogenetic analyses of haploid and allopolyploidPelliaspecies

Orzechowska

Siwinska

Małuszyńska

2010

Journal of Bryology

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“…Daniels, 1982;Wyatt, Stoneburner & Odrzykoski, 1989;Stoneburner, Wyatt & Odrzykoski, 1991;Wyatt, Odrzykoski & Stoneburner, 1992;Montagnes, Bayer & Vitt, 1993;Wyatt, 1994;Cronberg, 1996bCronberg, , 1998Cronberg, Molau & Sonesson, 1997;Akiyama, 1999;Appelgren & Cronberg, 1999). The reasoning is that selting ('automixis') is genetically equivalent to asexual reproduction in bryophytes.…”

Section: Introductionmentioning

confidence: 99%

Genetic variability in bryophytes: does mating system really matter?

Stenøien¹,

Såstad²

2001

Journal of Bryology

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Mating patterns do not alter allelic frequencies in natural populations. Therefore, if evolution is defined as changes in allelic frequencies in a population through time, then mating system is not an evolutionary force. There is no direct causal link between mating patterns and the familiar measures of genetic variability applied to allele frequency data from haploid gametophyte populations.In this paper we focus on how mating patterns may affect intralocus variability when acting together with evolutionary forces, like selection and drift. Moreover, we address how levels of inbreeding can have a profound influence on haplotypic variation. Measures for estimating the extent of recombination from haplotypic variation are presented. It seems that high levels of selting are not necessarily associated with bisexuality in bryophytes.

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“…Several authors have studied bryophyte isozymes in recent years (CUMMINS & WYATT, 1981;MEAGHER & SHAW, 1990;ODRZYKOSKI & SZWEYKOWSKI, 1991;WYATT et al, 1992;BISCHLER & BOISSELIER-DUBAYLE, 1993;MONTAGNES et al, 1993;MELICK et al, 1994;SHAW, 1994;SHAW et al, 1994 Colonies were stored at room temperature. An initial series of tests showed that there was no qualitative variation in isozyme activity in stems which had been dehydrated (3 months), nor after rehydration in distilled water for 30 min, 2 h, 48 h, or 192 h. All stems used in subsequent tests were always rehydrated for 18 h at room temperature.…”

Section: Electrophoretic Proceduresmentioning

confidence: 99%

“…An initial series of tests showed that there was no qualitative variation in isozyme activity in stems which had been dehydrated (3 months), nor after rehydration in distilled water for 30 min, 2 h, 48 h, or 192 h. All stems used in subsequent tests were always rehydrated for 18 h at room temperature. The leafy stem sections were ground in a mortar at 4°C (3 : 10; w/v) in the modified buffer solution of WYATT et al (1992) consisting of Tris-HCl (0.1 M, pH 7.5) containing EDTA (1 mM), ascorbic acid (2 mM), 2-mercaptoethanol (4 mM), sucrose (200 mM), MgCL, (10 mM), PVP 40 (2%) and KC1 (10 mM). The supernatant was collected after centrifuging for 10 min at 16,000 g. Glycerol (15%) was then added, and the extract used directly in the electrophoretic analyses.…”

Section: Electrophoretic Proceduresmentioning

confidence: 99%