Isozyme profiles associated with the hypersensitive response of Chenopodium foetidum to plum pox virus infection

Visedo, G.; Fernández‐Piqueras, José; Garcı́a, Juan Antonio

doi:10.1034/j.1399-3054.1990.780209.x

Cited by 7 publications

(17 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Presence of peroxidases would have to be virus induced, because the staining appears in infected apricot samples but not in controls. Induction of peroxidases by PPV has been previously described in Chenopodium foetidum (Visedo, Fernandez-Piqueras & Garcia, 1990) and Nicotiana cleuelandii (Visedo, Fernandez-Piqueras & Garcia, 1991). In peach GF305 seedlings this staining appeared in both inoculated and control samples.…”

Section: Discussionsupporting

confidence: 54%

Localisation of plum pox virus in apricot stem and petiole tissues by tissue printing onto nitrocellulose membrane

Dicenta

Audergon²

1995

Annals of Applied Biology

View full text Add to dashboard Cite

The localisation of plum pox virus (PPV) in stem and petiole tissues of nine susceptible apricot cultivars and GF305 peach seedling has been studied. From stem and petioles consecutive transverse sections spaced at 1 mm were made and tissue sections printed onto nitrocellulose membrane. The resulting prints were probed with a specific antibody for plum pox virus, followed by a rabbit anti-goat antibody conjugated with horse radish peroxidase, in order to localise the virus within the tissues. In stems the virus was mainly present in xylem and pith. The possible presence of the virus in the sclerenchyma is discussed. In petioles the virus was present in epidermis and parenchymas, but not in vessels. The probable movement through the xylem and from cell to cell has been shown.

show abstract

Section: Discussionsupporting

confidence: 54%

Localisation of plum pox virus in apricot stem and petiole tissues by tissue printing onto nitrocellulose membrane

Dicenta

Audergon²

1995

Annals of Applied Biology

View full text Add to dashboard Cite

show abstract

“…Isozyme profiles might be used as a reference when normal development of plants and pathological stages are compared, yielding valuable information about pathogenesis (Visedo et al 1990). No information about the effect of PPV infection on the activated oxygen-related enzymes in apricot plants is available.…”

Section: Introductionmentioning

confidence: 99%

Response of antioxidative enzymes to plum pox virus in two apricot cultivars

Hernández

Talavera

Martínez‐Gómez

et al. 2001

Physiologia Plantarum

View full text Add to dashboard Cite

Recent evidence has indicated that activated oxygen species (AOS) may function as molecular signals in the induction of defence genes. In the present work, the response of antioxidative enzymes to the plum pox virus (PPV) was examined in two apricot (Prunus armeniaca L.) cultivars, which behaved differently against PPV infection. In the inoculated resistant cultivar (Goldrich), a decrease in catalase (CAT) as well as an increase in total superoxide dismutase (SOD) and dehydroascorbate reductase (DHAR) activities were observed. Ascorbate peroxidase (APX), glutathione reductase (GR) and monodehydroascorbate reductase (MDHAR) did not change significantly in relation to non-inoculated (control) plants. In the susceptible cultivar (Real Fino), inoculation with PPV brought about a decrease in CAT, SOD and GR, whereas a rise in APX, MDHAR and DHAR activities was found in comparison to non-inoculated (control) plants. Apricot leaves contain only CuZn-SOD isozymes, which responded differently to PPV depending on the cultivar. Goldrich leaves contained 6 SODs and both SOD 1 and SOD 2 increased in the inoculated plants. In leaves from Real Fino, 5 SODs were detected and only SOD 5 was increased in inoculated plants. The different behaviour of SODs (H2O2-generating enzymes) and APX (an H2O2-remover enzyme) in both cultivars suggests an important role for H2O2 in the response to PPV of the resistant cultivar, in which no change in APX activity was observed. This result also points to further studies in order to determine if an alternative H2O2-scavenging mechanism takes place in the resistant apricot cultivar exposed to PPV. On the other hand, the ability of the inoculated resistant cultivar to induce SOD 1 and SOD 2 as well as the important increase of DHAR seems to suggest a relationship between these activities and resistance to PPV. This is the first report about the effect of PPV infection on the antioxidative enzymes of apricot plants. It opens the way for the further studies, which are necessary for a better understanding of the role of antioxidative processes in viral infection by PPV in apricot plants.

show abstract

“…at 4°C (Singh and Singh 1996). Electrophoresis was performed by the method of Visedo et al (1990). The enzyme extracts were loaded onto 3x8 mm filter paper wicks (Whatman No.…”

Section: Enzyme Extraction and Electrophoresismentioning

confidence: 99%

“…The method of Visedo et al (1990) was followed for naming the isozyme bands. Each band was named with a shortened form in capital letters (POX-for peroxidase) followed by a capital letter assigned in order from the most anodal to the most cathodal form of each enzyme.…”

Section: Nomenclaturementioning

confidence: 99%

Peroxidase isozyme profiles of immature and mature pollen of seven tropical plants from eastern India

Parui

Mondal

Mandal

1998

Grana

View full text Add to dashboard Cite

Isozyme profiles associated with the hypersensitive response of Chenopodium foetidum to plum pox virus infection

Cited by 7 publications

References 17 publications

Localisation of plum pox virus in apricot stem and petiole tissues by tissue printing onto nitrocellulose membrane

Localisation of plum pox virus in apricot stem and petiole tissues by tissue printing onto nitrocellulose membrane

Response of antioxidative enzymes to plum pox virus in two apricot cultivars

Peroxidase isozyme profiles of immature and mature pollen of seven tropical plants from eastern India

Contact Info

Product

Resources

About