Isozyme, Protein, and RAPD Markers within a Half‐Sib Family of Buffelgrass Segregating for Apospory

Buffel grass (Cenchrus ciliaris L.) is an apomictic species that is widely adapted to arid and semi-arid, tropical and warm climates. Six apomictic cultivars used as male parents, one sexual line used as the female parents and 15 putative F1 genotypes were studied using the amplified fragment length polymorphism (AFLP) technique for their evaluation and recognition. The NTSYS programme and Jaccard's Index were used to establish the genetic similarity; and a phenogram was constructed. A total of 152 bands was recorded. Three primer combinations were sufficient to identify the genotypes. The maximum proportion of polymorphisms was 0AE835, and 0AE510 of the polymorphisms consisted in individual bands that strictly diagnosed unique genotypes. Texas 4464 and Americana cultivars were the most different from the sexual line, indicating that they might be the best genotypes for future crossing. Two hybrids were identified among F1 material to demonstrate the usefulness of the AFLP technique in characterizing genotypes and recognizing hybrids in Buffel grass for breeding purposes.

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“…The DNA of each genotype was extracted from 0·5 g fresh leaf tissue of individual plants using the protocol of Gustine et al. (1996).…”

Section: Methodsmentioning

confidence: 99%

Molecular genetic discrimination of Buffel grass genotypes and F1 hybrids for breeding purposes using amplified fragment length polymorphism analyses

Griffa¹,

Díaz²,

Ribotta³

et al. 2006

Grass and Forage Science

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“…(Mennella et al, 1999). Total protein markers are also used extensively in characterization (Gustine et al, 1996;Mollema & Cole, 1996). In this study, 22 okra accessions were characterized based on their seed storage protein, total protein and isozyme (esterase) patterns to determine their suitability for any crop improvement programme.…”

Section: Introductionmentioning

confidence: 99%

Esterase, total protein and seed storage protein diversity in Okra (<i>Abelmoschus esculentus</i> L. Moench)

Torkpo

Danquah²,

Offei³

et al. 2009

West African Journal of Applied Ecology

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Twenty-two accessions of okra (Abelmoschus esculentus), maintained at the Plant Genetic Resources Centre, Bunso, Ghana, were assayed for diversity in esterases, and total and storage proteins. A total of 34 reproducible and easily scorable bands were exposed with the number of bands per accession ranging from one to 21. All but nine of the bands were polymorphic. Storage proteins were the most diverse while esterases revealed the least diversity. Similarity matrices were calculated using the Jaccard coefficient, and input into cluster analysis. The phenogram produced by the UPGMA of the Jaccard similarity matrix from the pooled data of the esterases, and total and storage proteins revealed three major clusters at the 55% level of similarity. Accession 5 collected from Nyinguto was relatively distant from the other main clusters and separated at the 42% level of similarity. The second and third clusters comprised 11 and 10 accessions, respectively. It was observed that 18 out of the 22 accessions were distinct accessions. Similarity indices ranged from 29% to 100%. The wide range of similarity indices, coupled with the clustering of accessions, suggests useful variability in the collection for genetic conservationists and plant breeders.

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“…Analyses of isozyme, protein, and RAPD markers did not identify associations with apospory in buffelgrass (Gustine et al, 1996). This can likely be attributed to the small population size and types of markers used.…”

mentioning

confidence: 99%

Disomic Inheritance, Suppressed Recombination, and Allelic Interactions Govern Apospory in Buffelgrass as Revealed by Genome Mapping

et al. 2002

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lecular markers within mapping populations (Wendel and Parks, 1984;Torres et al., 1985; Molecular tools have not identified the gene(s) governing apomixis Paterson et al., 1988Paterson et al., , 1991Saito et al., 1991; Lyt-nor have they been used to successfully transfer the trait to important, sexually reproducing food crops. Several molecular studies addressing tle, 1991;Schon et al., 1991; Zivy et al., 1992; Causse et apomixis in grasses have used interspecific and intergeneric hybrids. al., 1994; Chittenden et al., 1994). This loss of specific The failure to recover specific F 1 genotypes from these wide crosses genotypes from the progeny may be due to interactions can be caused by unfavorable interactions between the gametes, zybetween genes of the two species within gametes, zygote, embryo, endosperm, and/ or maternal tissue. These interactions gote, embryo, endosperm, or maternal tissue (Hadley can eliminate recombinant genotypes with valuable information toand Openshaw, 1980). Differential viability of offspring wards linkage analyses of the trait. Buffelgrass [Pennisetum ciliare because of allelic interactions has been reported in (L.) Link syn. Cenchrus ciliaris L.], a polymorphic species with intermaize (Zea mays L.)-Tripsacum hybrids (Maguire, fertile apomictic and sexual genotypes, offers an opportunity to genetically map apomixis by means of intraspecific hybrids with euploid 1963), tomato, Lycopersicon esculentum Mill. (Rick, genomes. This study reports a linkage map of the apospory region in 1966), wheat, Triticum aestivum L. (Manabe et al., 1999), buffelgrass. Apospory, classified by progeny testing and cytologically and rice,

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Isozyme, Protein, and RAPD Markers within a Half‐Sib Family of Buffelgrass Segregating for Apospory

Cited by 15 publications

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Molecular genetic discrimination of Buffel grass genotypes and F1 hybrids for breeding purposes using amplified fragment length polymorphism analyses

Molecular genetic discrimination of Buffel grass genotypes and F1 hybrids for breeding purposes using amplified fragment length polymorphism analyses

Esterase, total protein and seed storage protein diversity in Okra (<i>Abelmoschus esculentus</i> L. Moench)

Disomic Inheritance, Suppressed Recombination, and Allelic Interactions Govern Apospory in Buffelgrass as Revealed by Genome Mapping

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