Isozyme Variation and Species Delimitation in the Ceratocystis coerulescens Complex

Harrington, T. C.; Steimel, J.; Wingfield, M.J.; Kile, G. A.

doi:10.2307/3760789

Cited by 37 publications

(36 citation statements)

References 13 publications

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“…(24). A similar, and probablysynonymous fungus (Ceratocystis laricicola Redfern and Minter) has been isolated in low frequencies from the facultatively parasitic Ips cernbrae Heer (38)(39)(40). In the present study, C. polotzica, Ambrosiella sp., and 0. piceae induced phloem necrosis of comparable lengths.…”

Section: Discussionmentioning

confidence: 84%

Growth of four bark-beetle-associated blue-stain fungi in relation to the induced wound response in Norway spruce

Krokene¹,

Solheim²

1997

Can. J. Bot.

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Twenty-five-year-old Norway spruce trees (Picea abies) were inoculated with four blue-stain fungi. Each tree was inoculated three times with each fungus and three times with sterile agar as a control, giving a total of 15 inoculations per tree. There was little variation in the extent of phloem necrosis produced in response to the different fungi, but 5 weeks after inoculation necroses induced by Cerntocystis polorlica and Atnbrosiella sp. were significantly longer than those for the other fungi. At the same time, C. polonica had induced sapwood desiccation twice as deeply into the wood as any other fungus. Hyphal growth of the fungi into phloem and sapwood followed the same pattern as necrosis length and desiccation depth. Five weeks after inoculation, C. polorlica had penetrated phloem and sapwood farther than any other fungus. It grew more slowly than the other fungi in both tissues the first week after inoculation, but the four following weeks it grew more quickly than all other fungi.Resume : Les auteurs ont inocult des tpinettes de Norvkge (Picea nbies) iigtes de 25 ans, avec 4 champignons de la bleuissure du bois. 11s ont inocult chaque arbre trois fois avec cheque champignon, et trois fois avec de I'eau stCrile comme ttrnoins, ce qui a donnt 15 inoculations par abre. Il y a eu peu de variation dans I'Ctendue de la ntcrose du phloeme causte par les difftrents champignons, mais 5 semaines apres l'inoculation, les ntcroses induites par le Ceratocysris polor1ica avec I'Arnbrosiella sp. Ctaient significativement plus longues que celles caustes par les autres champignons. Simultantment le C. polonica a provoqut une dessiccation du bois deux fois plus profonde que tout autre champignon. La croissance des hyphes du champignon dans le phlottme et dans l'aubier suit le meme patron que la longueur de la nCcrose et la profondeur de la dessiccation. Cinq semaines aprks l'inoculation, le C. polorlicn avait pCnCtrC le phloeme et l'aubier plus profondtmcnt que les autres champignons. 11 pousse plus lentement que les autres champignons dans les deux tissus au cours des premieres semaines aprks I'inoculation, mais au cours des quatre semaines suivantes, il pousse plus rapidement que tous les autres.

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Section: Discussionmentioning

confidence: 84%

Growth of four bark-beetle-associated blue-stain fungi in relation to the induced wound response in Norway spruce

Krokene¹,

Solheim²

1997

Can. J. Bot.

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“…Harrington et al (1996) found that there were up to fi ve morphological variants of C. coerulescens isolates from conifers, and isozyme variations in the isolates supported distinguishing them as distinct taxa. Analysis of the DNA sequences of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA (Witthuhn et al 1998) .…”

Section: Discussionmentioning

confidence: 90%

“…are present in Japan. Ceratocystis coerulescens s.s. is considered a species more common as a bluestain fungus on dead wood or cut timber than as a wound colonizer of living trees (Harrington et al 1996;Harrington and Wingfi eld 1998). This fungus was isolated from the cutting face of Japanese larch logs left in the forest after thinning in Nagano Prefecture, Japan (unpublished data).…”

Section: Discussionmentioning

confidence: 99%

Constant association of ophiostomatoid fungi with the bark beetle Ips subelongatus invading Japanese larch logs

et al. 2009

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Ceratocystiopsis, Ceratocystis, Grosmannia, and Ophiostoma species were isolated from Ips subelongatus and beetle-infested Japanese larch logs collected at several areas in central and northern Honshu Island, Japan, to determine constant associates of I. subelongatus. Ceratocystiopsis minuta, two species of Ceratocystis, three species of Grosmannia, and four species of Ophiostoma were isolated. Of the fungi isolated in the present study, G. laricis, O. brunneociliatum, and O. piceae were constant associates of the beetles. Ceratocystis fujiensis, Ceratocystiopsis minuta, and Ophiostoma sp. F were occasionally isolated with high frequencies of occurrence but were not consistent associates. Ceratocystis fujiensis was most often isolated as the leading fungal invasion in the sapwood of Japanese larch logs invaded by I. subelongatus, confirming that the fungus acts as a primary invader of sapwood in beetle-attacked logs

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“…If partitiviruses have coevolved with their fungal hosts, it would be expected that sequence divergence of different partitiviruses would reflect the divergence of their fungal hosts. Although C. polonica and C. resinifera are morphologically similar, high variability in DNA sequences exist between these two species in the internal transcribed spacer (ITS) regions of the ribosomal RNA genes, and the species limits are quite clear (Harrington et al 1996;Witthuhn et al 1998Witthuhn et al , 2000. If the partitivirus in these two fungi arose from a common ancestor before the two fungal species diverged, a long period of coevolution with their fungal hosts would have resulted in a significant divergence of their dsRNA genome.…”

Section: Discussionmentioning

confidence: 98%

“…Fungi belonging to the Ceratocystis genus are the most frequently isolated from stained woods. Also, some Ceratocystis species can colonize living sapwood of wounded trees, and some are important symbionts of bark beetles that aid the beetles in killing trees (Harrington et al 1996;Paine et al 1997). In Canada, Ceratocystis resinifera Harrington & Wingfield is an important sapstaining fungus and causes significant damage to freshly cut wood.…”

Section: Introductionmentioning

confidence: 99%

Natural occurrence of a partitivirus in the sapstaining fungusCeratocystis resinifera

Deng

Boland

2007

Canadian Journal of Plant Pathology

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The bisegmented genome of a double-stranded RNA (dsRNA) virus from isolate LG115 of Ceratocystis resinifera, a sapstaining ascomycete fungus, was characterized. The larger segment (dsRNA-1) was 2305 base pairs in length, whereas the smaller one (dsRNA-2) was 2207 base pairs. The positive strand of dsRNA-1 contained an open reading frame (ORF) with the potential to encode a protein of 661 amino acids, and this ORF was closely related to coat proteins (CPs) of previously characterized fungal partitiviruses. The dsRNA-2 sequence encodes a putative protein of 663 amino acids, and this protein contains conserved motifs of RNA-dependent RNA polymerases (RDRPs) and is highly related to RDRPs of fungal partitiviruses. These results suggest that these two dsRNAs are the genome of a partitivirus. Nucleotide and amino acid comparisons revealed high similarities between this virus and a partitivirus described from Ceratocystis polonica, a closely related sapstaining fungus. Over the entire genome, the two viruses shared 82.8%-84.7% nucleotide sequence identities, and the amino acid sequence identities for CPs and RDRPs were 87.3% and 95.6%, respectively. These results suggest that the viruses in C. polonica and C. resinifera can be considered as two strains of the same partitivirus. The natural occurrence of a partitivirus in two fungal species indicates that horizontal transmission of this partitivirus may have occurred between these two fungi. Northern blot analysis indicated that this partitivirus was widespread in populations of C. resinifera. Deng and Boland: double-stranded RNA / Ceratocystis resinifera / partitivirus / mycovirus 189 Résumé : Le génome bisegmenté d'un virus à ARN bicaténaire provenant de l'isolat LG115 du Ceratocystis resinifera, un champignon ascomycète de coloration de la sève, a été caractérisé. Le plus grand segment (l'ARN-1 double brin) avait 2305 paires de bases alors que le plus petit (l'ARN-2 double brin) avait 2207 paires de bases. Le brin positif de l'ARN-1 double brin contenait un cadre de lecture ouvert potentiellement capable d'encoder une protéine de 661 acides aminés; ce cadre de lecture ouvert est très apparenté aux protéines de capside de partitivirus de champignons déjà caractérisées. L'ARN-2 double brin code pour une présumée protéine de 663 acides aminés; cette protéine contiendrait les motifs conservés de l'ARN-polymérase ARN-dépendante (APAD) des partitivirus de champignons et serait très apparentée à leurs APAD. Ces résultats portent à croire que ces deux ARN double brin constituent le génome d'un partitivirus. Les comparaisons de nucléotides et d'acides aminés révèlent de grandes similarités entre ce virus et un partitivirus provenant du Ceratocystis polonica, un champignon de coloration de sève très apparenté. Pour l'ensemble de leurs génomes, les deux virus partagent 82,8% à 84,7% de leurs séquences de nucléotides, alors que les similarités des séquences d'acides aminés sont respectivement de 87,3% et 95,6% pour les protéines de la capside et de l'APAD. Ces résultats laissent ...

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Isozyme Variation and Species Delimitation in the Ceratocystis coerulescens Complex

Cited by 37 publications

References 13 publications

Growth of four bark-beetle-associated blue-stain fungi in relation to the induced wound response in Norway spruce

Growth of four bark-beetle-associated blue-stain fungi in relation to the induced wound response in Norway spruce

Constant association of ophiostomatoid fungi with the bark beetle Ips subelongatus invading Japanese larch logs

Natural occurrence of a partitivirus in the sapstaining fungusCeratocystis resinifera

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