Charred olive wood is abundant in the archaeological record, especially around the Mediterranean. As the outermost ring closest to the bark is assumed to represent the latest time that the tree was alive, the radiocarbon date obtained from the outermost rings of an olive branch buried during the Santorini volcanic eruption is regarded as crucial evidence for the date of this cataclysmic event. The date of this eruption has far reaching consequences in the archaeology of the Aegean, Egypt and the Levant, and the understanding of their interconnections. We analyzed the radiocarbon concentrations in cross-sections from a modern olive tree trunk as well as from a living branch, and obtained near-annual resolution dates using the radiocarbon “bomb peak”. In both cases we show that radiocarbon dates of the last formed wood along the circumference are not chronologically homogenous, and can differ by up to a few decades. Thus the outermost wood layer does not necessarily represent the date of the last year of growth. These findings challenge the interpretation of the results obtained from dating the olive branch from the Santorini volcanic eruption, as it could predate the eruption by a few decades. In addition, our results are also significant for any future studies based on archaeologically preserved olive wood.