ISSR and SCoT for evaluation of hereditary differences of 29 wild plants in Al Jubail Saudi Arabian

Genetic variation of Amaranthus retroflexus L. and Chenopodium album L. (Amaranthaceae) suggests multiple independent introductions into Iran

“…Moreover based on PIC values, it can be concluded that the capacity of the marker system to detect polymorphic loci in a single amplification was very efficient; the average value of this coefficient amounted 0.78 for A. retroflexus L. and 0.71 for C. album L.. These results demonstrate this technique can be conveniently used for the genetic characterization of these populations of A. retroflexus L. and C. album L. Use of ISSR markers are also recently reported as a functional markers elsewhere (Sivaprakash et al, 2004;Yan et al, 2019;Kwiecińska-Poppe et al, 2020;Alotaibi and Abd-Elgawad, 2022;Flihi et al, 2022;Ghanbari et al, 2022;Haq et al, 2022).…”

“…ISSR markers are highly reproducible and accurate tools that generate highly reproducible banding patterns from a single polymerase chain reaction (PCR) amplification (Raut et al, 2014;Stefunova et al, 2014). Although newer technique are available, ISRR makers have historically (Wolfe et al, 1998) and recently (Yan et al, 2019;Kwiecińska-Poppe et al, 2020;Tang and Ma, 2020;Liu et al, 2021;Alotaibi and Abd-Elgawad, 2022;Flihi et al, 2022;Ghanbari et al, 2022;Haq et al, 2022) been used successfully for diversity studies and structuring of natural populations. Genomic DNA was isolated from the young leaves of plants according to the cetyltrimethylammonium bromide (CTAB) method described by Saghai-Maroof et al (1984).…”

Soil structure influences proteins, phenols, and flavonoids of varied medicinal plants in Al Jubail, KSA

“…The geographical area of the collection sites is cited in Fig. 1 ( Alotaibi and Abd-Elgawad, 2022 ). The median temperature ranged from 16 °C to 27 °C in day and night, respectively.…”

“…Variations in secondary metabolite composition and content can be found in a variety of medicinal plant species ( Ahn, 2017 ). Morphological, metabolic, and DNA-based polymorphs can be used to identify genetic variation among people or populations ( Alotaibi and Abd-Elgawad, 2022 , Goswami et al, 2021 ).…”

“…Few attempts have been made to investigate chemical and genetic variation among wild medicinal plant species using different molecular markers ( Alotaibi and Abd-Elgawad, 2022 , Abd-Elgawad and Alotaibi, 2017 ). Accordingly, the goal of the current study was to determine the impact of soil structure on the protein patterns, phenol, and flavonoid levels, as well as their correlation with the genetic similarity of Saudi Arabian plant species in Al Jubail.…”