ISSR-derived Molecular Markers for Korean Wheat Cultivar Identification

Abstract

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Inter simple sequence repeat (ISSR) is a technique that leads to the development of novel specific molecular markers and relationship analysis between species. In this study, 54 ISSRs were used to develop cultivar-specific DNA sequence characterized amplified regions (SCARs) for Korean wheat cultivars (Triticum aestivum). Using nine of 54 UBC ISSR primers, 16 cultivar-specific fragments were detected among 32 wheat cultivars. Of the 16 primers, 10 successfully amplified targets in 32 cultivars and six novel cultivar-specific markers were finally selected. A total of 13 Korean wheat cultivars (Ol, Dahong, Namhae, Jinpum, Anbaek, Jopoom, Jokyung, Sinmichal 1, Jeokjoong, Dajung, Goso and Joah) were classified by newly developed six SCAR markers. Cultivar-specific markers can be used not only for cultivar fingerprinting but also for marker-assisted selection in wheat breeding program. ISSR-derived Molecular Markers for Korean Wheat Cultivar Identification

Development of SCAR Markers for Korean Wheat Cultivars Identification

“…Six Korean wheat cultivar-specific marker sets have been developed from previous ISSR analysis (Son et al 2013). These markers can identify specifically the 13 Korean wheat cultivars from other cultivars (Son et al 2013).…”

“…Six Korean wheat cultivar-specific marker sets have been developed from previous ISSR analysis (Son et al 2013). These markers can identify specifically the 13 Korean wheat cultivars from other cultivars (Son et al 2013). But using KWSM007 and KWSM008 in addition to six ISSR-derived SCAR primer sets, we were able to distinguish 23 out of 32 Korean wheat cultivars (Table 1).…”

“…A total of 13 Korean cultivars were classified using the six SCAR markers sets developed in Son et al (2013). Using this study as reference two SCAR markers KWSM007 and KWSM008 were added to the set of markers we developed.…”

“…The DNA band of 215 bp length was amplified by KWSM007 primer pairs in ten cultivars including Eunpa, Olgeuru, Gobun, Saeol, Milsung, Sinmichal, Jokyung, Sugang, Goso, and Joah. Then KWSM007 primer was applied to the six ISSR-derived SCAR primer sets (Son et al 2013) as newly SCAR marker which enabled the identification of seven other cultivars (Table 1; Fig 2). In other words, 20 out of 32 Korean wheat cultivars were classified by combination of seven SCAR primer sets containing KWSM007 primer.…”

“…In addition, AFLP technique was used for mutation analysis caused by transposition, insertion and deletion of some nucleotide in genome as well as it has been used for the analysis of genetic map (Mackill et al 1996;Voorrips et al 1997), evolution (Heun et al 1997) and research of bio-diversity (Barrett and Kidwell 1998). Similar to RAPDs or inter simple sequence repeats (ISSRs), bands of the species-specific obtained by AFLP can be converted into SCARs (Son et al 2013). Thus, AFLP technique is an important tool with variety of applications.…”