ISSR primer screening for analysis of genetic diversity among <i>Scutellaria tuvensis</i> (Lamiaceae) populations

Muraseva, Dinara S.; Гусева, А. А.

doi:10.1051/bioconf/20213800082

Cited by 3 publications

(2 citation statements)

References 3 publications

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“…Since DNA methylation, amplification, activation of transposable elements, polyploidy, changes in chromosome number or DNA sequence can be affected by in vitro culture conditions and consequently lead to genetic changes in regenerated plants [ 27 , 28 ], it is necessary to monitor the occurrence of somaclonal variations during in vitro propagation and assess the genetic constitution and variability of the plants regenerated in vitro. The genetic diversity was successfully detected in the genus Scutellaria and mainly concerned the assessment of the genetic diversity of the study’s wild and cultivated populations using RAPD markers [ 29 , 30 , 31 ], ISSR markers [ 32 , 33 ], and SSR (simple sequence repeat) markers [ 34 ]. The use of the aforementioned markers to assess the variability of Baikal skullcap genotypes obtained using a tissue culture has been reported sporadically in the literature, but SCoT-type markers were used for the first time in this study.…”

Section: Resultsmentioning

confidence: 99%

Potential of In Vitro Culture of Scutellaria baicalensis in the Formation of Genetic Variation Confirmed by ScoT Markers

Gawroński

Dyduch‐Siemińska

2022

Genes

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The in vitro culture technique can be used for micropropagation of medicinal plants as well as for creating genotypes with an improved profile of phytochemical compounds. For this purpose, somaclonal variability may be used for the induction of genetic diversity among regenerants. The paper presents a protocol for obtaining Scutellaria baicalensis regenerants by indirect organogenesis and the assessment of their genetic variability with the use of start codon-targeted markers. The most intense process of indirect shoot organogenesis was observed on Murashige and Skoog medium supplemented with kinetin and 6-Benzylaminopurine (0.5 mg × dm−3 each)—7.4 shoot per explant on average. The callogenesis process occurred on the medium supplemented with TDZ, while the medium supplemented with GA3 allowed for direct shoot organogenesis and was used for the micropropagation of regenerants. In the analysis of plantlets obtained by indirect organogenesis, 11 ScoT markers generated a total of 130 amplicons, 45 of which were polymorphic. This analysis showed genetic diversity of regenerants in relation to the donor plant as well as within them, with mean similarity among the analyzed genotypes at the level of 0.90. This study confirms that the use of in vitro cultures allows for the possibility to generate genetic variability in Scutellaria baicalensis, which can be effectively revealed with the use of the SCoT marker.

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Section: Resultsmentioning

confidence: 99%

Potential of In Vitro Culture of Scutellaria baicalensis in the Formation of Genetic Variation Confirmed by ScoT Markers

Gawroński

Dyduch‐Siemińska

2022

Genes

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“…For PCR, the DNA concentration of each sample was adjusted to 10.0 ng/µL. Twenty-two ISSR primers were screened, and 10 most informative ones were selected [32].…”

Section: Molecular Analysismentioning

confidence: 99%

Morphological and Genetic Diversity of Scutellaria tuvensis Juz., an Endemic of Desert Steppes

Гусева

Muraseva

Cheryomushkina

2022

IJPB

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For the first time, an assessment of phenotypic variability and genetic polymorphism was performed on endemic plants Scutellaria tuvensis Juz. growing in Tuva (five populations; Russia). Based on morphological traits of individuals, principal component analysis clustered the individuals into three groups depending on characteristics of their habitats: group 1 turned out to be sampled from beach gravel, group 2 from a detrital cone, and group 3 from coarse rock fragments; this finding was confirmed by specific features of the development of the individuals in these habitats. Using inter-simple sequence repeat markers, high genetic polymorphism was identified at the population level: the proportion of polymorphic loci was 95%, expected heterozygosity 0.221, the absolute number of alleles 1.533, and the effective number of alleles 1.376. Population 3 (P 3) was the most genetically homogeneous; P 5 was characterized by the highest genetic diversity. In an unweighted pair group method with arithmetic mean dendrogram, the studied populations formed two major groups: the first cluster included P 4 and P 5, and the second cluster contained plants collected in P 1, P 2, and P 3. An analysis of the population structure using the STRUCTURE software showed the same result, dividing the sample under study into two subpopulations. The genetic differentiation index among populations was 0.232, and gene flow 1.655. According to analysis of molecular variance, intrapopulation differences accounted for 73% of total genetic diversity.

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Heteroblastic Inflorescence of Lamium amplexicaule L. in Egyptian Flora

Amer

Shaye

Hassan

et al. 2023

Plants

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Lamium amplexicaule L. (Family: Lamiaceae) is a cosmopolitan weed whose eradication is challenging. The phenoplasticity of this species is related to its heteroblastic inflorescence, which has not received adequate research worldwide in its morphological and genetic aspects. This inflorescence hosts two flower types, a cleistogamous (CL: closed flower) and a chasmogamous (CH: opened flower). This species subjected to detailed investigation is a model species to clarify: (1) the existence of the CL and CH flowers in relation to the time and individual plants. (2) the predominant flower morphs in Egypt. (3) the morphological and genetic variability between these morphs. Among the novel data retrieved from this work is the Presence of this species in three distinct morphs coexisting during winter. These morphs showed remarkable phenoplasticity, particularly in flower organs. Significant differences were observed between the three morphs in pollen fertility, nutlets productivity and sculpture, flowering time, and seed viability. These differences were extended to the genetic profile of these three morphs assessed by the inter simple sequence repeats (ISSRs) and start codon targeted (SCoT). This work highlights the urgent need to study the heteroblastic inflorescence of crop weeds to facilitate its eradication.

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ISSR primer screening for analysis of genetic diversity among Scutellaria tuvensis (Lamiaceae) populations

Cited by 3 publications

References 3 publications

Potential of In Vitro Culture of Scutellaria baicalensis in the Formation of Genetic Variation Confirmed by ScoT Markers

Potential of In Vitro Culture of Scutellaria baicalensis in the Formation of Genetic Variation Confirmed by ScoT Markers

Morphological and Genetic Diversity of Scutellaria tuvensis Juz., an Endemic of Desert Steppes

Heteroblastic Inflorescence of Lamium amplexicaule L. in Egyptian Flora

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