Issues determining direct airways hyperresponsiveness in mice

Lundblad, Lennart K. A.

doi:10.3389/fphys.2012.00408

Cited by 11 publications

(18 citation statements)

References 70 publications

(85 reference statements)

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“…[12][13][14][15][16] Because of the advantages of oscillometry, it was speculated that it might become the new standard by which lung function is assessed and, at the same time, increase our understanding of respiratory diseases in a way similar to how it revolutionized animal research. 17 Oscillometry is also an example where the same physiological measurements can be made in animal models as in human patients. Respiratory oscillometry has become the de facto standard technique used in the animal laboratory, 17 and the heterogeneity seen in allergic mouse lungs is similar to what is seen in human lungs as exemplified in Dr. Dandurand's section in this article.…”

Section: Introductionmentioning

confidence: 99%

Applications of oscillometry in clinical research and practice

Lundblad

Siddiqui

Bossé

et al. 2019

Canadian Journal of Respiratory, Critical Care, and Sleep Medic

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Oscillometry is gaining in clinical use and while there is an increased interest in the technique, there is paucity in the understanding of its possibilities and limitations. Oscillometry has seen extensive use in research over several decades, but only recently is the technique being adopted in clinical practice; hence, there is a need to educate the novel users. The goal of the mini symposium arranged in San Diego in 2018 was to discuss the principles of oscillometry, showcase some of the recent ongoing research using this technique and to demonstrate how oscillometry may be used in clinical practice. It was concluded that oscillometry has several advantages over spirometry, most notably, with novel data being shown, its sensitivity allowing early detection of small airways disease not possible with spirometry and it can be used in subjects who have difficulties performing forced maneuvers such as preschool children, the elderly and subjects with handicaps. The site of respiratory pathology can be reflected by the various parameters generated by oscillometry and thus help with both disease diagnosis and localization. While the interpretation of oscillometry parameters and translating them into meaningful pathological correlates is still evolving, it is likely that oscillometry will soon be at the forefront of both pulmonary clinical practice and research. R ESUM EL'oscillom etrie est de plus en plus utilis ee en contexte clinique, mais malgr e l'int erêt accru pour cette technique, la compr ehension de ses possibilit es et de ses limites est encore insuffisante. Pendant plusieurs d ecennies, l'oscillom etrie a largement et e utilis ee dans la recherche, mais l'adoption de cette technique dans la pratique clinique est encore r ecente, de sorte qu'il est n ecessaire de former les nouveaux utilisateurs. Le but de ce mini symposium qui s'est tenu a San Diego en 2018 etait de discuter des principes de l'oscillom etrie, de pr esenter les plus r ecentes etudes utilisant cette technique et de d emontrer comment elle peutêtre utilis ee dans la pratique clinique. Il a et e d etermin e que l'oscillom etrie pr esentait plusieurs avantages par rapport a la spirom etrie notamment, comme le d emontrent de nouvelles donn ees, sa sensibilit e qui permet la d etection pr ecoce de la maladie des petites voies a eriennes, ce que la spirom etrie ne peut pas faire, ainsi que le fait qu'elle puisseêtre utilis ee aupr es de sujets qui eprouvent de la difficult e a effectuer des manoeuvres forc ees comme les enfants d'âge pr escolaire, les personnesâg ees et les personnes atteintes d'un handicap. L'emplacement de la pathologie respiratoire peut se refl eter dans les divers param etres produits par la spirom etrie, ce qui peut contribuer a diagnostiquer la maladie et a la localiser. Bien que l'interpr etation des param etres de l'oscillom etrie et leur traduction dans des corr elats pathologiques significatifs continue d' evoluer, l'oscillom etrie sera vraisemblablement a l'avant-plan de la pratique clinique et de la recherche pulmonair...

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Section: Introductionmentioning

confidence: 99%

Applications of oscillometry in clinical research and practice

Lundblad

Siddiqui

Bossé

et al. 2019

Canadian Journal of Respiratory, Critical Care, and Sleep Medic

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, while care should be taken not to over-interpret these results until further validation is provided, it should also be noted that strategies are being proposed to increase the translational value of preclinical murine models. These include steps directed at standardizing lung function measurement techniques as well as introducing novel methods of assessing or reporting lung function, to which the present study adds (Stevenson et al, 2013;Walker et al, 2012;Lundblad 2012;Robichaud et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Mechanical consequences of allergic induced remodeling on mice airway resistance and compressibility

Novali

Shalaby

Robichaud³

et al. 2015

Respiratory Physiology & Neurobiology

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“…Although this approach is widely accepted, it has an important weakness, in that results are comparable only if identical protocols are used. While steps were recently made to standardize this widely used technique, as well as to increase the translational utility of AHR measurements in mice (22,36), variations in the aerosol generation or transport aspect of the protocol are common. The rationale for such variations is not disputed, as they may be required to address specific scientific questions.…”

Section: Discussionmentioning

confidence: 99%

“…However, no standardized procedure exists; therefore, protocol variations can frequently be found in the literature, even when the same measurement technique is used. Close scrutiny of a list of research articles where the forced oscillation technique, an invasive measurement approach referred by some as the "gold standard" for respiratory mechanics assessment (22), was used to evaluate AHR to aerosolized methacholine in mice commonly reveals variations affecting the aerosol generation or delivery aspect of the protocol (1,3,16,17,25,29,30,(33)(34)(35)37). For example, in the latter selection of articles, at least four different nebulizers were used, nebulization time varied from 4 to 90 s, and changes in the ventilation profile or modifications to the inspiratory line were sometimes introduced.…”

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confidence: 99%

Delivered dose estimate to standardize airway hyperresponsiveness assessment in mice

Robichaud¹,

Fereydoonzad²,

Schuessler³

2015

American Journal of Physiology-Lung Cellular and Molecular Phys

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Airway hyperresponsiveness often constitutes a primary outcome in respiratory studies in mice. The procedure commonly employs aerosolized challenges, and results are typically reported in terms of bronchoconstrictor concentrations loaded into the nebulizer. Yet, because protocols frequently differ across studies, especially in terms of aerosol generation and delivery, direct study comparisons are difficult. We hypothesized that protocol variations could lead to differences in aerosol delivery efficiency and, consequently, in the dose delivered to the subject, as well as in the response. Thirteen nebulization patterns containing common protocol variations (nebulization time, duty cycle, particle size spectrum, air humidity, and/or ventilation profile) and using increasing concentrations of methacholine and broadband forced oscillations (flexiVent, SCIREQ, Montreal, Qc, Canada) were created, characterized, and studied in anesthetized naïve A/J mice. A delivered dose estimate calculated from nebulizer-, ventilator-, and subject-specific characteristics was introduced and used to account for protocol variations. Results showed that nebulization protocol variations significantly affected the fraction of aerosol reaching the subject site and the delivered dose, as well as methacholine reactivity and sensitivity in mice. From the protocol variants studied, addition of a slow deep ventilation profile during nebulization was identified as a key factor for optimization of the technique. The study also highlighted sensitivity differences within the lung, as well as the possibility that airway responses could be selectively enhanced by adequate control of nebulizer and ventilator settings. Reporting results in terms of delivered doses represents an important standardizing element for assessment of airway hyperresponsiveness in mice.

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Issues determining direct airways hyperresponsiveness in mice

Cited by 11 publications

References 70 publications

Applications of oscillometry in clinical research and practice

Applications of oscillometry in clinical research and practice

Mechanical consequences of allergic induced remodeling on mice airway resistance and compressibility

Delivered dose estimate to standardize airway hyperresponsiveness assessment in mice

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