Itaconic acid production from glycerol using <i>Escherichia coli</i> harboring a random synonymous codon‐substituted 5′‐coding region variant of the <i>cad</i>A gene

Jeon, Ho Geun; Cheong, Dae Eun; Han, Yong; Song, Jae Jun; Choi, Jong Hyun

doi:10.1002/bit.25914

Cited by 41 publications

(32 citation statements)

References 22 publications

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“…1). Other studies have indicated the importance of cadA expression for itaconate production1920. E. coli BL21(DE3) containing acn and three cadA genes yielded a much higher conversion (64.0%).…”

Section: Discussionmentioning

confidence: 95%

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Production of itaconate by whole-cell bioconversion of citrate mediated by expression of multiple cis-aconitate decarboxylase (cadA) genes in Escherichia coli

Kim

Seo

Bhatia

et al. 2017

Sci Rep

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Itaconate, a C5 unsaturated dicarboxylic acid, is an important chemical building block that is used in manufacturing high-value products, such as latex and superabsorbent polymers. Itaconate is produced by fermentation of sugars by the filamentous fungus Aspergillus terreus. However, fermentation by A. terreus involves a long fermentation period and the formation of various byproducts, resulting in high production costs. E. coli has been developed as an alternative for producing itaconate. However, fermentation of glucose gives low conversion yields and low productivity. Here, we report the whole-cell bioconversion of citrate to itaconate with enhanced aconitase and cis-aconitate decarboxylase activities by controlling the expression of multiple cadA genes. In addition, this bioconversion system does not require the use of buffers, which reduces the production cost and the byproducts released during purification. Using this whole-cell bioconversion system, we were able to catalyze the conversion of 319.8 mM of itaconate (41.6 g/L) from 500 mM citrate without any buffer system or additional cofactors, with 64.0% conversion in 19 h and a productivity of 2.19 g/L/h. Our bioconversion system suggests very high productivity for itaconate production.

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Section: Discussionmentioning

confidence: 95%

“…produced itaconate using an E. coli strain expressing heterologous cadA , achieving a titer of 690 mg/L18. Another E. coli strain with random synonymous codon substitutions produced 7.23 g/L of itaconate19. Model-based metabolic engineering of E. coli increased itaconate production to 32 g/L20.…”

mentioning

confidence: 99%

Production of itaconate by whole-cell bioconversion of citrate mediated by expression of multiple cis-aconitate decarboxylase (cadA) genes in Escherichia coli

Kim

Seo

Bhatia

et al. 2017

Sci Rep

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“…Recently, Yang et al [102] reported that co-expression of aconitase (ACO) and CAD in E. coli (uCA) resulted in the production of 117.2 mg IA L −1 after 48 h fermentation while the IA titer was significantly improved to 222.1 mg L −1 by self-assembly of ACO-PDZ (APd) and CAD-PDZlig (CPI) in E. coli (sPP) under the same conditions. Jeon et al [35] reported IA production from glycerol using E. coli harboring a random synonymous codon-substituted 5ʹ-coding region variants of the cadA gene. They showed that functional expression of the cadA gene was improved through synonymous codon substitution at the first ten codons (excluding ATG).…”

Section: Recombinant Microorganisms For Production Of Itaconic Acidmentioning

confidence: 99%

Emerging biotechnologies for production of itaconic acid and its applications as a platform chemical

Saha¹

2017

Journal of Industrial Microbiology and Biotechnology

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Recently, itaconic acid (IA), an unsaturated C5-dicarboxylic acid, has attracted much attention as a biobased building block chemical. It is produced industrially (>80 g L) from glucose by fermentation with Aspergillus terreus. The titer is low compared with citric acid production (>200 g L). This review summarizes the latest progress on enhancing the yield and productivity of IA production. IA biosynthesis involves the decarboxylation of the TCA cycle intermediate cis-aconitate through the action of cis-aconitate decarboxylase (CAD) enzyme encoded by the CadA gene in A. terreus. A number of recombinant microorganisms have been developed in an effort to overproduce it. IA is used as a monomer for production of superabsorbent polymer, resins, plastics, paints, and synthetic fibers. Its applications as a platform chemical are highlighted. It has a strong potential to replace petroleum-based methylacrylic acid in industry which will create a huge market for IA.

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“…It may be due to the high shear rate generated by mechanical agitation in the STR that may damage fungal cells. Optimum balance between aeration rate and agitation speed needs to be maintained to avoid mechanical stress on the mycelia caused by excessive impeller speed, or conversely poor oxygen transfer rate at lower speed. Hence, agitation and aeration rates were simultaneously varied in the current investigation to find the optimum balance.…”

Section: Discussionmentioning

confidence: 99%

Optimization and scale up of itaconic acid production from potato starch waste in stirred tank bioreactor

Bafana

Sivanesan

Pandey

2019

Biotechnology Progress

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Present study used Aspergillus terreus strain C1 isolated from mangrove soil for itaconic acid (IA) production from potato starch waste. Fermentation parameters were optimized by classical one factor approach and statistical experimental designs, such as Plackett-Burman and response surface designs. Anionic deionization of potato waste was found to be a very effective, economic, and easy way of improving IA production. The increase in IA production by deionization was found to correlate with removal of phosphate. In our knowledge, this is the first report on application of deionization of potato waste to enhance IA production. Other parameters like inoculum development conditions, pH, presence of peptone and certain salts in the medium also significantly affected IA production. IA production by strain C1 increased 143-fold during optimization when compared with the starting condition. The optimized IA level (35.75 g/L) was very close to the maximum production predicted by RSM (38.88 g/L). Bench scale production of IA was further optimized in 3-L stirred tank reactor by varying parameters like agitation and aeration rate. The maximum IA production of 29.69 g/L was obtained under the agitation speed of 200 rpm and aeration rate of 0.25 vvm. To the best of our knowledge, it is the first report on IA production from potato starch waste at bioreactor level.

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Itaconic acid production from glycerol using Escherichia coli harboring a random synonymous codon‐substituted 5′‐coding region variant of the cadA gene

Cited by 41 publications

References 22 publications

Production of itaconate by whole-cell bioconversion of citrate mediated by expression of multiple cis-aconitate decarboxylase (cadA) genes in Escherichia coli

Production of itaconate by whole-cell bioconversion of citrate mediated by expression of multiple cis-aconitate decarboxylase (cadA) genes in Escherichia coli

Emerging biotechnologies for production of itaconic acid and its applications as a platform chemical

Optimization and scale up of itaconic acid production from potato starch waste in stirred tank bioreactor

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