Italy enacts new law on medically assisted reproduction

Boggio, Andrea

doi:10.1093/humrep/deh871

Cited by 69 publications

(38 citation statements)

References 7 publications

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“…The ability to efficiently cryopreserve human oocytes will not only assist many female patient groups, including those who are preparing to undergo fertility-threatening radiotherapy or chemotherapy (Falcone et al, 2004) but will also have an impact on practical aspects of human IVF. Examples include the inability to collect a sperm sample on the day of oocyte retrieval, permitting robust viral infection testing during oocyte donation and storage of supernumerary oocytes for patients in countries that only allow the insemination of a limited number of oocytes (Boggio, 2005;Li et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

1,2-propanediol and the type of cryopreservation procedure adversely affect mouse oocyte physiology

Larman¹,

Katz-Jaffe²,

Sheehan³

et al. 2006

Human Reproduction

113

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BACKGROUND: The aim of this work was to examine the effect of 1,2-propanediol (PrOH) and type of cryopreservation procedure (slow freezing and vitrification) on oocyte physiology. METHODS: Intracellular calcium of mouse metaphase II (MII) oocytes was quantified by fluorescence microscopy. The effect of PrOH on cell physiology was further assessed through analysis of zona pellucida hardening and cellular integrity. Protein profiles of cryopreserved oocytes were generated by time-of-flight mass spectrometry (TOF-MS). RESULTS: PrOH caused a protracted increase in calcium, which was sufficient to induce zona pellucida hardening and cellular degeneration. Using 'nominally calcium free' media during PrOH exposure significantly reduced the detrimental effects. Proteomic analysis identified numerous up-and down-regulated proteins after slow freezing when compared with control and vitrified oocytes. CONCLUSIONS: Using such approaches to assess effects on cellular physiology is fundamental to improving assisted reproduction techniques (ART). This study demonstrates that PrOH causes a significant rise in intracellular calcium. Using calcium-free media significantly reduced the increase in calcium and the associated detrimental physiological effects, suggesting that calcium-free media should be used with PrOH. In addition, analysis of the oocyte proteome following cryopreservation revealed that slow freezing has a significant effect on protein expression. In contrast, vitrification had a minimal impact, indicating that it has a fundamental advantage for the cryopreservation of oocytes.

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Section: Introductionmentioning

confidence: 99%

1,2-propanediol and the type of cryopreservation procedure adversely affect mouse oocyte physiology

Larman¹,

Katz-Jaffe²,

Sheehan³

et al. 2006

Human Reproduction

113

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“…In February 2004February , a law (n. 40/2004) regulating assisted reproduction techniques (ARTs) was approved in Italy (Boggio, 2005;Conti and Delbon, 2005). Even though this is the first attempt to regulate the treatment of infertility in Italy, it contains several prohibitions and restrictions.…”

Section: Introductionmentioning

confidence: 99%

Clinical use of the perifollicular vascularity assessment in IVF cycles: a pilot study

Palomba¹,

Russo²,

Falbo³

et al. 2005

Human Reproduction

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BACKGROUND:In Italy, a recent law has imposed a ban on the fertilization of more than three oocytes at one time, and all resulting embryos produced must be transferred simultaneously. The aim of the present controlled study was to assess the clinical feasibility and efficacy of the perifollicular vascularity assessment for oocyte selection in IVF cycles. METHODS: Fifty-four young primary infertile non-obese women (27 cases and 27 age-and BMI-matched controls) underwent IVF cycles. The choice of the oocytes to fertilize was performed according to perifollicular vascularization in the experimental group, whereas in the control group, the standard morphologic criteria alone were used. The dose of gonadotrophins used, the dominant follicles obtained, the duration of the ovarian stimulation, the number of oocytes retrieved, the number/quality of oocytes fertilized and of cleaved embryos, cycle cancellation, implantation, clinical pregnancy, ongoing pregnancy, multiple pregnancies and ovarian hyperstimulation syndrome rates were assessed in each group. RESULTS: The assessment of perifollicular vascularity was feasible in 88.9% of cases. No difference between groups was detected in any parameter evaluated. CONCLUSION: Power Doppler assessment of perifollicular vascularity seems to have no clinical utility for oocyte selection in IVF cycles for young infertile women.

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“…Recently, reproductive medicine has been enriched by techniques and scientific contributions in favour of infertile couples and their right to become parents, although there is a different legislation among countries to regulate the activities of the infertility centers [1,2]. In this regard, we considered a couple to be potentially infertile when reproductive difficulties for a period >12 months occurred, while regularly practising unprotected sexual intercourse [3].…”

Section: Introductionmentioning

confidence: 99%

SIAMS survey on sexological screening during the assisted reproductive technologies in Italy

Ciocca

Limoncin

Mollaioli

et al. 2015

J Endocrinol Invest

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Italy enacts new law on medically assisted reproduction

Cited by 69 publications

References 7 publications

1,2-propanediol and the type of cryopreservation procedure adversely affect mouse oocyte physiology

1,2-propanediol and the type of cryopreservation procedure adversely affect mouse oocyte physiology

Clinical use of the perifollicular vascularity assessment in IVF cycles: a pilot study

SIAMS survey on sexological screening during the assisted reproductive technologies in Italy

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