Iterative In Situ Click Chemistry Creates Antibody‐like Protein‐Capture Agents

Agnew, Heather D.; Rohde, Rosemary D.; Millward, Steven W.; Nag, Arundhati; Yeo, Woon‐Seok; Hein, Jason E.; Pitram, Suresh M.; Tariq, Abdul; Burns, Vanessa M.; Krom, Russell J; Fokin, Valery V.; Sharpless, K. Barry; Heath, James R.

doi:10.1002/ange.200900488

Cited by 42 publications

(45 citation statements)

References 31 publications

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“…Owing to the semiselective nature of the interaction of AMPs with pathogenic bacteria, differentiation of multiple species of pathogenic bacteria has not been achieved. Future work will involve exploring strategies to improve this selectivity via investigations into multi-ligand 53,54 and aptamer-based capture agents 26,55 , and antibody-based biorecognition molecules with improved stability 56 to provide stringent discrimination between species of pathogenic bacteria. Alternative strategies for covalent and non-covalent functionalization of graphene sensors will also be explored 57 .…”

Section: Discussionmentioning

confidence: 99%

Graphene-based wireless bacteria detection on tooth enamel

et al. 2012

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Direct interfacing of nanosensors onto biomaterials could impact health quality monitoring and adaptive threat detection. Graphene is capable of highly sensitive analyte detection due to its nanoscale nature. Here we show that graphene can be printed onto water-soluble silk. This in turn permits intimate biotransfer of graphene nanosensors onto biomaterials, including tooth enamel. The result is a fully biointerfaced sensing platform, which can be tuned to detect target analytes. For example, via self-assembly of antimicrobial peptides onto graphene, we show bioselective detection of bacteria at single-cell levels. Incorporation of a resonant coil eliminates the need for onboard power and external connections. Combining these elements yields two-tiered interfacing of peptide-graphene nanosensors with biomaterials. In particular, we demonstrate integration onto a tooth for remote monitoring of respiration and bacteria detection in saliva. overall, this strategy of interfacing graphene nanosensors with biomaterials represents a versatile approach for ubiquitous detection of biochemical targets.

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Section: Discussionmentioning

confidence: 99%

Graphene-based wireless bacteria detection on tooth enamel

et al. 2012

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“…First, the application of microfluidic approaches to make many, inexpensive and rapid measurements from small amounts of blood is required-and several new approaches are being developed in this regard. Second, new specific protein-capture agents, are required to provide the essential molecular identifiers, currently only provided by antibodies 4 . The use of antibodies must be replaced because of the difficulty in obtaining a large number of highly specific, tight-binding antibodies, and the fragility of these molecules in extensive everyday use.…”

Section: Synopsismentioning

confidence: 99%

Systems Biology and Emerging Technologies Will Catalyze the Transition from Reactive Medicine to Predictive, Personalized, Preventive and Participatory (P4) Medicine

Galas¹,

Hood²

2009

Interdisciplinary Bio Central

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SYNOPSISWe stand at the brink of a fundamental change in how medicine will be practiced. Over the next 5-20 years medicine will move from being largely reactive to being predictive, personalized, preventive and participatory (P4). Technology and new scientific strategies have always been the drivers of revolutions and this is certainly the case for P4 medicine, where a systems approach to disease, new and emerging technologies and powerful computational tools will open new windows for the investigation of disease. Systems approaches are driving the emergence of fascinating new technologies that will permit billions of measurements on each individual patient. The challenge for health information technology will be how to reduce this enormous amount of data to simple hypotheses about health and disease. We predict that emerging technologies, together with the systems approaches to diagnosis, therapy and prevention will lead to a down turn in the escalating costs of healthcare. In time we will be able to export P4 medicine to the developing world and it will become the foundation of global medicine. The -democratization‖ of healthcare will come from P4 medicine. Its first real emergence will require the unprecedented integration of biology, medicine, technology and computationas well as societal issues of major importance: ethical, regulatory, public policy, economic, and others. In order to effectively move the P4 scientific agenda forward new strategic partnerships are now being created with the large-scale integration of complementary skills, technologies, computational tools, patient records and samples and analysis of societal issues. It is evident that the business plans of every sector of the healthcare industry will need to be entirely transformed over the next 10 years-and the extent to which this will be done by existing companies as opposed to newly created companies is a fascinating question.

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“…For a highly multiplexed conventional (antibody-based) protein assay, the major costs are the antibodies that are used to capture and detect specific proteins. Alternative protein capture agents 40,41 that show the selectivity and sensitivity of antibodies, but also exhibit chemical, biochemical and physical stability, would provide a transformative technology that would influence all assay platforms, from conventional to nanotechnology-based.…”

Section: Selected Health Applicationsmentioning

confidence: 99%

Nanoscience and Technology

Chen¹

2009

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Iterative In Situ Click Chemistry Creates Antibody‐like Protein‐Capture Agents

Cited by 42 publications

References 31 publications

Graphene-based wireless bacteria detection on tooth enamel

Graphene-based wireless bacteria detection on tooth enamel

Systems Biology and Emerging Technologies Will Catalyze the Transition from Reactive Medicine to Predictive, Personalized, Preventive and Participatory (P4) Medicine

Nanoscience and Technology

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