ITPP Treatment of RG2 Glioblastoma in a Rat Model

The Allosteric Hemoglobin Effector ITPP Inhibits Metastatic Colon Cancer in Mice

“…Importantly, ITPP treatment improves survival of cancer-bearing animals, particularly if combined with cytotoxic agents. [16][17][18][19][20][21][22][23] Recently, we observed a prolonged survival through ITPP in a mouse model of established CRC liver metastasis, with a marked potentiation of efficacy when combined with FOL-FOX. 16,24 However, whether ITPP can counteract the spread of CRC to distant sites is unknown.…”

Failure of Inositol Trispyrophosphate to Enhance Highly Effective Radiotherapy of GL261 Glioblastoma in Mice

“…Mice tended to die sooner, and with smaller tumors, when they had received ITPP. After performing these experiments, we became aware of the study of Förnvik et al showing no benefit of ITPP monotherapy in the treatment of orthotopic syngeneic rat RG2 glioblastoma, and a possible negative survival impact of ITPP on rats injected with RG2 subcutaneously (24). Although RT was not used in that study, the results support the conclusion that ITPP is not a promising monotherapy for glioblastoma.…”

Body weight algorithm predicts humane endpoint in an intracranial rat glioma model

“…Except for weight, however, most criteria that have been reported in this context are qualitative and highly observer-dependent 7 . Also, authors often do not differentiate whether rats were euthanized or died spontaneously 8,9 . The Council Directive 2010/63/EU requires that animals must be sacrificed at the earliest possible time point whenever death is foreseeable to reduce the duration and intensity of suffering.…”

“…(a) Body weight change in per cent compared to d-8; significant differences were found between the day of the endpoint compared to all other days and between the second last day compared to day -6, -5 and -4 (F (8,144) = 33.769, p < 0.001). (b) Clinical score was increased on the last two days compared to all previous days (F(8,144) = 195,069, p < 0.001). (c) Burrowed gravel in gram (g) was decreased on the day of the endpoint compared to all other days and on the second last day compared to day -6 (F (8,96) = 14.937, p < 0.001).…”