iTRAQ-based comparative proteomic analysis in mature leaves of Ethiopian mustard (Brassica carinata) provides insights into the whole-proteomic profiles and the role of anthocyanin biosynthesis in leaf color diversity

Abstract

Background Anthocyanins are the main pigments in leaves, flowers and fruits, performing diverse biological functions in plants and exhibiting benefits for human health. The purple-leaved accession of B. carinata was used to study the biosynthetic mechanism of anthocyanins in this study. To elucidate the mechanisms of anthocyanin accumulation in the purple-leaved line, we employed a proteomic approach based on an isobaric tags for relative and absolute quantification (iTRAQ)-based technique to investigate the protein expression profile of the purple-leaved line of B. carinata in comparison with that of the green-leaved line.Results In total, 4,631 proteins were identified, of which 285 exhibited significant changes in abundance between BC-G01 and BC-P01. Of the 285 DEPs, 175 are upregulated and 110 are downregulated in the leaves of the purple-leaved line compared with the leaves of the green-leaved line. Bioinformatics analysis indicated that anthocyanin biosynthesis was the most significantly elevated metabolic process, containing three DEPs corresponding to two genes UGT75C1 and UGT79B1 . UGT75C1 and UGT79B1 , encoding anthocyanin 3-O-glucoside: 2’’-O-xylosyltransferase and anthocyanin 5-O-glucosyltransferase, respectively, play an important role in the biosynthesis of anthocyanins.Conclusions The exact functions of these proteins remain to be examined. Nevertheless, our study paves the way for understanding the genetic regulatory mechanisms of anthocyanin biosynthesis and accumulation in B. carinata .

Frontiers in Dissecting and Managing Brassica Diseases: From Reference-Based RGA Candidate Identification to Building Pan-RGAomes

“…B. napus , or canola, is the third most important oilseed crop globally following soybean and palm [ 6 ]. B. juncea is cultivated as an oilseed, spice, vegetable and fodder crop species [ 7 ] and B. carinata originated from the Ethiopian plateau and is an important condiment, vegetable and oilseed crop in northeast Africa [ 8 ].…”