iTRAQ-Based Identification of Proteins Related to Muscle Growth in the Pacific Abalone, Haliotis discus hannai

Abstract

The abalone Haliotis discus hannai is an important aquaculture species that is grown for human consumption. However, little is known of the genetic mechanisms governing muscle growth in this species, particularly with respect to proteomics. The isobaric tag for relative and absolute quantitation (iTRAQ) method allows for sensitive and accurate protein quantification. Our study was the first to use iTRAQ-based quantitative proteomics to investigate muscle growth regulation in H. discus hannai. Among the 1904 proteins identified from six samples, 125 proteins were differentially expressed in large specimens of H. discus hannai as compared to small specimens. In the large specimens, 47 proteins were upregulated and 78 were downregulated. Many of the significant Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including these differentially expressed proteins, were closely related to muscle growth, including apoptosis, thyroid hormone signaling, regulation of the actin cytoskeleton, and viral myocarditis (p < 0.05). Our quantitative real-time polymerase chain reaction (qRT-PCR) analyses suggested that the alterations in expression levels observed in the differentially expressed proteins were consistent with the alterations observed in the encoding mRNAs, indicating the repeatability of our proteomic approach. Our findings contribute to the knowledge of the molecular mechanisms of muscle growth in H. discus hannai.

Expression profiling of lncRNAs and mRNAs reveals regulation of muscle growth in the Pacific abalone, Haliotis discus hannai

“…Three of the samples were larger (“L_HD” group; mean weight, 95.1 ± 7.7 g; mean muscle weight, 45.5 ± 5.0 g), and three were smaller (“S_HD” group; mean weight, 16.5 ± 1.0 g; mean muscle weight, 7.3 ± 0.8 g). All six specimens of the adductor muscle, mantle, visceral mass, and gill were collected from each abalone, immediately snap-frozen in liquid nitrogen 46 .…”

iTRAQ-Based Identification of Proteins Related to Lignin Synthesis in the Pear Pollinated with Pollen from Different Varieties

“…In previous studies, proteomic profiling has been shown to be a direct reflection of protein expression patterns that provides information about protein regulation and active pathways [ 15 ].…”

Molecular Characterization and Function of Bone Morphogenetic Protein 7 (BMP7) in the Pacific Abalone, Haliotis discus hannai

“…The H. discus hannai is the most popular cultivated abalone throughout in China [ 41 , 42 ]. The abalone growth cycle generally lasts for 2 years, which restricts the development of abalone aquaculture.…”