iTRAQ-based proteomic analysis reveals the mechanisms of Botrytis cinerea controlled with Wuyiencin

Shi, Liming; Ge, Beibei; Wang, Jinzi; Liu, Binghua; Ma, Jinjin; Wei, Qiuhe; Zhang, Kecheng

doi:10.1186/s12866-019-1675-4

Cited by 11 publications

(9 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using 2D-E in combination with IgE-immunoblotting, more than 20 allergenic proteins of fungal spores could be identified in the airborne mold A. versicolor (e.g., glyceraldehyde-3-phosphate dehydrogenase, catalase A, enolase) and a component-resolved allergen testing could be obtained using patients’ serum IgEs [ 262 ]. Shi et al [ 263 ] used iTRAQ to characterize the biological actions of wuyiencin treatment on the grey mold Botrytis cinerea , common cause of plant disease, and found evidence of this biological control agent to debilitate the growth and pathogenicity of the fungus. Both ultrastructural alterations and changes in protein abundance were observed and the latter concerned the downregulation of proteins involved in amino acid, protein and nucleotide biosynthesis, a phenomenon frequently observed also during starvation of pathogenic fungi.…”

Section: Proteomics Advances In Ascomycotamentioning

confidence: 99%

An Overview of Genomics, Phylogenomics and Proteomics Approaches in Ascomycota

Muggia

Ametrano

Sterflinger

et al. 2020

Life

View full text Add to dashboard Cite

Fungi are among the most successful eukaryotes on Earth: they have evolved strategies to survive in the most diverse environments and stressful conditions and have been selected and exploited for multiple aims by humans. The characteristic features intrinsic of Fungi have required evolutionary changes and adaptations at deep molecular levels. Omics approaches, nowadays including genomics, metagenomics, phylogenomics, transcriptomics, metabolomics, and proteomics have enormously advanced the way to understand fungal diversity at diverse taxonomic levels, under changeable conditions and in still under-investigated environments. These approaches can be applied both on environmental communities and on individual organisms, either in nature or in axenic culture and have led the traditional morphology-based fungal systematic to increasingly implement molecular-based approaches. The advent of next-generation sequencing technologies was key to boost advances in fungal genomics and proteomics research. Much effort has also been directed towards the development of methodologies for optimal genomic DNA and protein extraction and separation. To date, the amount of proteomics investigations in Ascomycetes exceeds those carried out in any other fungal group. This is primarily due to the preponderance of their involvement in plant and animal diseases and multiple industrial applications, and therefore the need to understand the biological basis of the infectious process to develop mechanisms for biologic control, as well as to detect key proteins with roles in stress survival. Here we chose to present an overview as much comprehensive as possible of the major advances, mainly of the past decade, in the fields of genomics (including phylogenomics) and proteomics of Ascomycota, focusing particularly on those reporting on opportunistic pathogenic, extremophilic, polyextremotolerant and lichenized fungi. We also present a review of the mostly used genome sequencing technologies and methods for DNA sequence and protein analyses applied so far for fungi.

show abstract

Section: Proteomics Advances In Ascomycotamentioning

confidence: 99%

An Overview of Genomics, Phylogenomics and Proteomics Approaches in Ascomycota

Muggia

Ametrano

Sterflinger

et al. 2020

Life

View full text Add to dashboard Cite

show abstract

“…In addition, an iTRAQ-based LC-MS/MS analysis was used to evaluate the proteomic profiling of B. cinerea in response to wuyiencin, produced by Streptomyces albulus subsp. wuyiensis , widely used as an antifungal agent in agriculture [ 17 ]. The high-throughput capability of mass spectrometry analyses was exploited to evaluate proteomic changes occurring within Penicillium species following treatment with plant-derived pesticides.…”

Section: Discussionmentioning

confidence: 99%

Deciphering Molecular Determinants Underlying Penicillium digitatum’s Response to Biological and Chemical Antifungal Agents by Tandem Mass Tag (TMT)-Based High-Resolution LC-MS/MS

Citores

Valletta

Singh

et al. 2022

IJMS

View full text Add to dashboard Cite

Penicillium digitatum is a widespread pathogen responsible for the postharvest decay of citrus, one of the most economically important crops worldwide. Currently, chemical fungicides are still the main strategy to control the green mould disease caused by the fungus. However, the increasing selection and proliferation of fungicide-resistant strains require more efforts to explore new alternatives acting via new or unexplored mechanisms for postharvest disease management. To date, several non-chemical compounds have been investigated for the control of fungal pathogens. In this scenario, understanding the molecular determinants underlying P. digitatum’s response to biological and chemical antifungals may help in the development of safer and more effective non-chemical control methods. In this work, a proteomic approach based on isobaric labelling and a nanoLC tandem mass spectrometry approach was used to investigate molecular changes associated with P. digitatum’s response to treatments with α-sarcin and beetin 27 (BE27), two proteins endowed with antifungal activity. The outcomes of treatments with these biological agents were then compared with those triggered by the commonly used chemical fungicide thiabendazole (TBZ). Our results showed that differentially expressed proteins mainly include cell wall-degrading enzymes, proteins involved in stress response, antioxidant and detoxification mechanisms and metabolic processes such as thiamine biosynthesis. Interestingly, specific modulations in response to protein toxins treatments were observed for a subset of proteins. Deciphering the inhibitory mechanisms of biofungicides and chemical compounds, together with understanding their effects on the fungal physiology, will provide a new direction for improving the efficacy of novel antifungal formulations and developing new control strategies.

show abstract

“…Plant pathogens such as fungi, nematodes, bacteria, and viruses cause massive crop losses or damage worldwide. − Among these diseases, pathogenic fungi cause significant postharvest loss in fruits and vegetables, making it a pressing public concern and a research priority. − Botrytis cinerea (B. cinerea), a necrotrophic fungus, causes enormous economic losses during crop maturity and harvest. − B.…”

Section: Introductionmentioning

confidence: 99%

Tavaborole-Induced Inhibition of the Aminoacyl-tRNA Biosynthesis Pathway against Botrytis cinerea Contributes to Disease Control and Fruit Quality Preservation

Zhao

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

The inhibitory effect of tavaborole on the invasion of Botrytis cinerea in grapes and tomatoes, as well as the potential mechanism involved, was discovered in this study. Our findings showed that tavaborole inhibited Botrytis cinerea spore germination and mycelial expansion in vitro and that the control efficiency in vivo on fruit decay was dose-dependent, which was effective in reducing disease severity and maintaining the organoleptic quality of the fruit, such as reducing weight loss and retaining fruit hardness and titratable acid contents during storage. Furthermore, the precise mechanism of action was investigated further. Propidium iodide staining revealed that Botrytis cinerea treated with tavaborole lost membrane integrity. For further validation, cytoplasmic malondialdehyde accumulation and leakage of cytoplasmic constituents were determined. Notably, the inhibitory effect was also dependent on inhibiting the activities of aminoacyl-tRNA synthetases involved in the aminoacyl-tRNA biosynthesis pathway in Botrytis cinerea. The above findings concluded that tavaborole was effective against Botrytis cinerea infection in postharvest fruit, and a related mechanism was also discussed, which may provide references for the drug repurposing of tavaborole as a postharvest fungicide.

show abstract

iTRAQ-based proteomic analysis reveals the mechanisms of Botrytis cinerea controlled with Wuyiencin

Cited by 11 publications

References 46 publications

An Overview of Genomics, Phylogenomics and Proteomics Approaches in Ascomycota

An Overview of Genomics, Phylogenomics and Proteomics Approaches in Ascomycota

Deciphering Molecular Determinants Underlying Penicillium digitatum’s Response to Biological and Chemical Antifungal Agents by Tandem Mass Tag (TMT)-Based High-Resolution LC-MS/MS

Tavaborole-Induced Inhibition of the Aminoacyl-tRNA Biosynthesis Pathway against Botrytis cinerea Contributes to Disease Control and Fruit Quality Preservation

Contact Info

Product

Resources

About