iTRAQ-Based Proteomic Profile Analysis of the Hepatopancreas of Caribbean Spiny Lobsters Infected With Panulirus argus Virus 1: Metabolic and Physiological Implications

Zamora‐Briseño, Jesús Alejandro; Ruiz‐May, Eliel; Elizalde‐Contreras, José M.; Hernández-Velázquez, Ioreni Margarita; Hernández-Pérez, Ariadne; Fuentes-García, Ana Guadalupe; Herrera-Salvatierra, Nancy; Briones‐Fourzán, Patricia; Pascual-Jímenez, Cristina; Lozano‐Álvarez, Enrique; Rodríguez-Canul, Rossanna

doi:10.3389/fmicb.2020.01084

Cited by 6 publications

(3 citation statements)

References 93 publications

(131 reference statements)

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“…In recent proteomic analyses, we analyzed molecular changes in lobsters naturally infected (PaV1) by isobaric tags for relative and absolute quantitation (iTRAQ) [ 37 ], in which a total of 636 proteins were identified, with 68 down-regulated and 71 up-regulated proteins. Among the down-regulated proteins, several enzymes involved in the metabolism of hormones, lipids and digestive enzymes were identified, while proteins associated with the histone core, protein synthesis, immune response and RNA regulation were up-regulated.…”

Section: Discussionmentioning

confidence: 99%

“…Sample portions of hepatopancreas, gills, muscle, midgut, and heart were fixed in Davidson’s AFA fixative (alcohol, formalin, acetic acid) to determine the severity of PaV1-infection. Tissue were processed, embedded, sectioned (thickness: 5–6 µm), and stained with hematoxylin and eosin (H&E) or Pinkerton’s stain, following routine histological protocols [ 36 , 37 ]. Sections were analyzed to determine histopathological changes.…”

Section: Methodsmentioning

confidence: 99%

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Immune Response to Natural and Experimental Infection of Panulirus argus Virus 1 (PaV1) in Juveniles of Caribbean Spiny Lobster

Pascual-Jímenez

Rodríguez-Canul

Huchin-Mian

et al. 2022

Animals

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Experimental infections have been used to better comprehend the immune system of organisms, and to probe for additives that generate greater resistance and help reduce antibiotic use in aquaculture. We compared the immune response of juveniles of the Caribbean spiny lobster, Panulirus argus, infected naturally with Panulirus argus virus 1 (PaV1) versus organisms infected experimentally, to determine the analogy between both infectious processes. The immunological response was measured by hemagglutination activity, hemocyte count, and total phenoloxidase activity in plasma and hemocytes in 211 individuals that were either naturally infected (110), or had been injected with viral inoculum and followed for six months (101). The samples were classified into the following four groups according to the severity of the infection: 0, uninfected; 1, lightly; 2, moderately; and 3, severely infected), which was determined on the basis of PCR and histological criteria. A permutational MANOVA showed that both the origin (natural and experimental), and the severity of the infection contributed significantly to explain the variation in the immune response of lobsters. The lack of significance of the interaction term indicated that the immunological response changed with the severity of the infection in a similar way, regardless of its origin. The results of the present study suggest that the experimental viral infection of PaV1 produces a defense response similar to the natural pathways of contagion, and provides the bases to validate an immunological challenge protocol for the first time in crustaceans. The discussion includes the perspective of the conceptual models of immune response within an ecological context.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Immune Response to Natural and Experimental Infection of Panulirus argus Virus 1 (PaV1) in Juveniles of Caribbean Spiny Lobster

Pascual-Jímenez

Rodríguez-Canul

Huchin-Mian

et al. 2022

Animals

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“…The expression levels of selected DEGs were normalized by comparison to MTB2 internal reference genes. Values for Ct were exported, the fold-change was estimated as relative gene expression levels using the ddCt method, transformed to log2 scale, and compared with transcriptome data [ 67 , 68 ].…”

Section: Methodsmentioning

confidence: 99%

Comparative Transcriptomes of the Body Wall of Wild and Farmed Sea Cucumber Isostichopus badionotus

Martín‐Hernández

Rodríguez-Canul²,

Kantún-Moreno³

et al. 2021

IJMS

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Overfishing of sea cucumber Isostichopus badionotus from Yucatan has led to a major population decline. They are being captured as an alternative to traditional species despite a paucity of information about their health-promoting properties. The transcriptome of the body wall of wild and farmed I. badionotus has now been studied for the first time by an RNA-Seq approach. The functional profile of wild I. badionotus was comparable with data in the literature for other regularly captured species. In contrast, the metabolism of first generation farmed I. badionotus was impaired. This had multiple possible causes including a sub-optimal growth environment and impaired nutrient utilization. Several key metabolic pathways that are important in effective handling and accretion of nutrients and energy, or clearance of harmful cellular metabolites, were disrupted or dysregulated. For instance, collagen mRNAs were greatly reduced and deposition of collagen proteins impaired. Wild I. badionotus is, therefore, a suitable alternative to other widely used species but, at present, the potential of farmed I. badionotus is unclear. The environmental or nutritional factors responsible for their impaired function in culture remain unknown, but the present data gives useful pointers to the underlying problems associated with their aquaculture.

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Crustaceans

Hancock-Ronemus¹,

Buote²,

Lewbart³

2021

Invertebrate Medicine

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iTRAQ-Based Proteomic Profile Analysis of the Hepatopancreas of Caribbean Spiny Lobsters Infected With Panulirus argus Virus 1: Metabolic and Physiological Implications

Cited by 6 publications

References 93 publications

Immune Response to Natural and Experimental Infection of Panulirus argus Virus 1 (PaV1) in Juveniles of Caribbean Spiny Lobster

Immune Response to Natural and Experimental Infection of Panulirus argus Virus 1 (PaV1) in Juveniles of Caribbean Spiny Lobster

Comparative Transcriptomes of the Body Wall of Wild and Farmed Sea Cucumber Isostichopus badionotus

Crustaceans

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