iTRAQ-based quantitative proteomic analyses the cycle chronic heat stress affecting liver proteome in yellow-feather chickens

Abstract

Heat stress ( HS ) is one of the main environmental factors affecting the efficiency of poultry production. The yellow-feather chickens ( YFC ) as an indigenous strain of chicken is a popular poultry breed in China. Our previous study used the RNA-seq to analyze the gene expression profiles of male YFC under HS and showed that the lipid and energy metabolism pathways are activated in livers of YFC exposed to acute HS (38°C, 4 h and 25°C recovery 2 h). In this study, we used quantitative proteome analysis based on iTRAQ to study the liver response of YFC to cycle chronic HS (38 ± 1°C, 8 h/d, 7 d, CyCHS ). The male YFCs treatment used the CyCHS from 22 to 28 days of age. The liver tissue samples were collected at 28 d old. A total of 39,327 unique peptides matches were detected using iTRAQ analysis and 4,571 proteins exhibited a false discovery rate of 1% or less. Forty-six significant differentially expressed proteins ( DEPs ) were detected in the CyCHS group compared with the control group for the liver samples, including up- and down-regulated DEPs were 18 and 28, respectively. We found that the enriched biological process terms of the DEPs expressed in the liver were related to DNA metabolic process, oxidation-reduction process, oxidative stress and gluconeogenesis. In KEGG pathway analysis. Most of the hepatic DEPs were annotated to glutathione metabolism and TCA cycle in response to CyCHS. The up-regulation of 5 DEPs (GPX1, GSTT1, GSTT1L, RRM2, and LOC100859645) in the glutathione metabolism pathway likely reflects an attempt to deal with oxidative damage by CyCHS. The down-regulation of 3 DEPs (Isocitrate dehydrogenase [ IDH3A ], IDH3B, and phosphoenolpyruvate carboxykinase 1) in the TCA cycle pathway contributes to the regulation mechanism of energy metabolism and probably to cope with the balance of heat production and dissipation during CyCHS in order to adapt to high temperature environments. Our results provide insights into the potential molecular mechanism in heat-induced oxidative stress and energy in YFCs and future studies will investigate the functional genes associated with the response to HS.

Whole genome discovery of regulatory genes responsible for the response of chicken to heat stress

“…In addition, almost 90% of them (121 out of the 134 mRNAs) were found to be regulated by 216 miRNAs. According to the previous studies and the findings of the current work, the expression of several important genes are modified under HS; including HSPA5 12 , 36 , 72 , 75 , 76 , EIF2A 77 – 79 , RBPJ 80 , ABCC9 81 , TAT 82 , 83 , ATP5J2 84 , CD80 85 , EEF2K 86 , EIF4B 87 , FTO 88 , 89 , GLRX 90 , IGF1R 91 , INSR 77 , 92 , VAV3 93 , UBR5 94 , TNIP1 90 , PTPN9 95 , EIF2A 96 , AKR1D1 97 , HSPA13 98 , CYP1A2 99 , and SDF2L1 12 . Additionally, based on our result, other protein-coding genes such as ABCC9, TAT, ATP5J2, CD80, EEF2K, EIF4B, FTO, GLRX, IGF1R, INSR, VAV3, UBR5, TNIP1, RBPJ, and PTPN9, were significantly modified with the heat stress in conjunction with the regulation effect of ENSGALT00000099876, ENSGALT00000106323, and ENSGALT00000107573 lncRNAs.…”

The effect of epigallocatechin gallate on laying performance, egg quality, immune status, antioxidant capacity, and hepatic metabolome of laying ducks reared in high temperature condition

“…Liver is an important organ in bird that maintains nutritional homeostasis, produces circulatory proteins, coordinates transitions related to adaptation in carbohydrate, lipid, and protein metabolism (Zhang et al. 2021 ). Hepatic antioxidants consist of antioxidative enzymes, and defensive proteins such as heat shock proteins (Hsps) (Surai et al.…”

Effects of the In ovo Administration of the 6/85 Mycoplasma gallisepticum Vaccine on Layer Chicken Embryo Hatchability and Early Posthatch Performance

“…Nevertheless, in ovo administration of MDV has been shown to provide stronger protection to hatching broilers when injected in ovo in the amnion (AM) rather than in the air cell (AC) [ 42 , 43 ]. Moreover, the in ovo injection of L-ascorbic acid in the AC has been shown to increase hatchling quality [ 51 ] and the antioxidant capacity of 28-day-old broilers [ 52 ]. Thus, the different injected materials used between the studies could be the reason for the inconsistent results between the AC and AM injections.…”