iTRAQ comparison of proteomic profiles of endometrial receptivity

Pérez-Debén, Silvia; Bellver, José; Alamá, Pilar; Salsano, Stefania; Quiñonero, Alicia; Sebastián-León, Patricia; Díaz-Gimeno, Patricia; Domı́nguez, Francisco

doi:10.1016/j.jprot.2019.103381

Cited by 18 publications

(9 citation statements)

References 71 publications

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“…The results for ANT3, alpha-1-acid glycoprotein (P02763), vitamin D-binding protein (P02774), and FETUA (P02765) were also consistent with previous studies on the menstrual phase (DeSouza et al, 2005). Ribosomal proteins and apolipoproteins have also been identified in ours and other previous studies (DeSouza et al, 2005;Domínguez et al, 2009;Pérez-Debén et al, 2019).…”

Section: Discussionsupporting

confidence: 85%

Peer Review #1 of "Screening and identification of endometrial proteins as novel potential biomarkers for repeated implantation failure (v0.2)"

Marron¹

2021

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Inadequate endometrial receptivity may be responsible for the low implantation rate of transferred embryos in in vitro fertilization (IVF) treatments. Patients with repeated implantation failure (RIF) impact the clinical pregnancy rate for IVF. We collected endometrial tissue during the implantation window of hysteroscopy biopsies from September 2016 to December 2019 and clinical data were collected simultaneously.Patients were divided into RIF and pregnant controls group according to pregnancy outcomes. A total of 82 differentially expressed endometrial proteins were identified, including 55 up-regulated proteins (>1.50-fold, P<0.05) and 27 down-regulated proteins (<0.67-fold, P<0.05) by iTRAQ labeling coupled with the 2D LC MS/MS technique in the RIF group. String analysis found interactions between these proteins which assembled in two bunches: ribosomal proteins and blood homeostasis proteins. The most significant enriched Gene Ontology terms were negative regulation of hydrolase activity, blood microparticle, and enzyme inhibitor activity. Our results emphasized the corticosteroidbinding globulin and fetuin-A as the specific proteins of endometrial receptivity by Western-blot. Our study provided experimental data to establish the objective indicator of endometrial receptivity, and also provided new insight into the pathogenesis of RIF.

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Section: Discussionsupporting

confidence: 85%

Peer Review #1 of "Screening and identification of endometrial proteins as novel potential biomarkers for repeated implantation failure (v0.2)"

Marron¹

2021

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“…Various cytokines, such as IFN-γ, IL-10a, and IL-6 have been shown to regulate ribosomes through receptors, thereby affecting macrophage metabolism as well as mRNA translation (28). Silvia et al (29) evaluated endometrial receptivity-related pathways and found that the ribosomal pathway is the most relevant for endometrial fertility. Furthermore, the result of GSEA showed a negative correlation between RPS9 and proteasome.…”

Section: Discussionmentioning

confidence: 99%

Artificial Neural Network Model Using Immune-infiltration Modules for Endometrial Receptivity Assessment of Implantation Failure

Duan

Sha

et al. 2021

Preprint

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ObjectivesThis study was anchored on the state of local immune-infiltration in the endometrium, which acts as critical factors affecting embryonic implantation, and aimed at establishing novel approaches to assess endometrial receptivity for patients with IVF failure.MethodsImmune-infiltration levels in the GSE58144 dataset (n=115) from GEO were analyzed by digital deconvolution and validated by immunofluorescence (n=30), illustrating that dysregulation of the ratio of Mf1 to Mf2 is an important factor contributing to implantation failure. Then, modules most associated with M1/M2 macrophages (Mfs) and their hub genes were then selected by weighted gene co-expression network and univariate analyses, then validated by GSE5099 macrophage dataset, qPCR analysis (n=16), and western blot. It revealed that closely related gene modules dominated three biological processes in macrophages: antigen presentation, interleukin−1−mediated signalling pathway, and phagosome acidification, respectively. Their hub genes were significantly altered in patients and related with ribosomal, lysosome, and proteasomal pathways. Finally, the artificial neural network (ANN) and nomogram models were established from hub genes, of which efficacy was compared and validated in the GSE165004 dataset (n=72). Models established by the selected hub genes exhibited excellent predictive values in both datasets, and ANN performed best with an accuracy of 98.3% and an AUC of 0.975 (95% CI 0.945-1). ConclusionsMacrophages, proven to be essential for endometrial receptivity, were regulated by gene modules dominating antigen presentation, interleukin−1−mediated signalling pathway, and phagosome acidification. Selected hub genes can effectively assess endometrial dysfunction receptivity for IVF outcomes by the ANN approach.

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“…The 100 μg peptide was extracted from the sample and labeled as per the instructions in the ABSCIEX iTRAQ Labeling Kit (Henan, China). 7 High PH: Reverse chromatography grading Each set of labeled peptides was mixed and graded using the Agilent 1260 Infinity II high-performance liquid chromatography (HPLC) system (Agilent Technologies, Santa Clara, CA, USA). Buffer A was 10 mM HCOONH4, 5% acetonitrile (ACN), pH 10.0, and buffer B was 10 mM HCOONH4, 85% ACN, pH 10.0.…”

Section: Itraq Labelmentioning

confidence: 99%

Detection of serum major histocompatibility complex I (HLA‐1) and β2‐microglobulin (β2M) in pre‐eclampsia using isobaric tags for relative and absolute quantitation (iTRAQ)

Shi,

Zhang,

Liu

et al. 2023

Intl J Gynecology & Obste

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ObjectiveThe purpose of this preliminary investigation into the pathogenesis of pre‐eclampsia was to screen the differential proteins in the serum of pregnant women with normal pregnancy and early‐onset pre‐eclampsia using isobaric tags for relative and absolute quantitation (iTRAQ), so as to identify serum biomarkers for the early diagnosis of pre‐eclampsia.MethodsWe examined the peripheral serum of 58 normal pregnant women and 42 pregnant women with early‐onset pre‐eclampsia using iTRAQ; the differentially expressed proteins were screened for bioinformatics analysis; and the expression of candidate proteins human leukocyte antigen‐1 (HLA‐1) and β2‐microglobulin (β2M) in placental tissues was detected using western blot.ResultsWe identified a total of 63 differential proteins in the serum of patients from the normal control group and the pre‐eclampsia group, and this included 24 up‐regulated proteins and 39 down‐regulated proteins. The western blot results of placental tissue showed reduced HLA‐1 expression (1.12 ± 0.23) in the placenta in the pre‐eclampsia group as compared with the normal control group (1.34 ± 0.22). Consistent with the results observed in the serum, β2M in the placenta in the pre‐eclampsia group was significantly elevated (1.05 ± 0.47) in comparison with the normal group (0.75 ± 0.33) (P < 0.05).ConclusionIn this study, we found that iTRAQ technology was useful for identifying differentially expressed proteins in the peripheral serum of pregnant women with pre‐eclampsia, and that HLA‐1 and β2M, which may be involved in the occurrence of pre‐eclampsia, show promise as predictive markers of pre‐eclampsia.

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iTRAQ comparison of proteomic profiles of endometrial receptivity

Cited by 18 publications

References 71 publications

Peer Review #1 of "Screening and identification of endometrial proteins as novel potential biomarkers for repeated implantation failure (v0.2)"

Peer Review #1 of "Screening and identification of endometrial proteins as novel potential biomarkers for repeated implantation failure (v0.2)"

Artificial Neural Network Model Using Immune-infiltration Modules for Endometrial Receptivity Assessment of Implantation Failure

Detection of serum major histocompatibility complex I (HLA‐1) and β2‐microglobulin (β2M) in pre‐eclampsia using isobaric tags for relative and absolute quantitation (iTRAQ)

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