iTRAQ proteomics reveals changes in the lettuce (Lactuca sativa L. Grand Rapid) proteome related to colour and senescence under modified atmosphere packaging

Abstract

BACKGROUND This study used the isobaric tags for relative and absolute quantitation (iTRAQ) technique to determine the effects of active modified atmosphere packaging (MAP) on the modulation of lettuce (Lactuca sativa L. Grand Rapid) colour and senescence. Lettuces, treated or not treated (control) with MAP, were placed in a refrigerator (4 °C, 90–95% relative humidity) for analysis at 3‐day intervals until the ninth day. RESULTS MAP treatment could suppress weight loss, loss of green colour and brittleness, and browning of lettuce; at the same time, MAP preserved the green colour of lettuce by inhibiting chlorophyll degradation and chloroplast disruption, and delayed lettuce senescence by inhibiting malondialdehyde (MDA) generation and increasing antioxidant enzyme activity. In total, 285 differentially abundant proteins (144 upregulated proteins and 141 downregulated proteins) were identified in MAP‐treated and non‐treated lettuce. Further analysis revealed that MAP regulated the expression of proteins involved in photosynthesis (e.g. pheophorbide a oxygenase, PaO; porphobilinogen deaminase, PBGD), and thus preserved lettuce colour. The iTRAQ analysis also showed that MAP regulated the expression of antioxidant enzymes and reduced the accumulation of reactive oxygen species, and thus delayed lettuce senescence. CONCLUSION MAP can maintain vegetable quality by inhibiting chlorophyll degradation, delaying vegetable senescence. © 2018 Society of Chemical Industry

Preservation of Quality and Bioactive Compounds in Mangoes Using Chitosan-Graphene-Oxide-Based Biodegradable Packaging

“…β-carotene is a pro-vitamin A lipophilic molecule with important biological functions related to antioxidant activity, providing protection to human organism against cancer, cardiovascular diseases and diabetes by scavenging the oxygen free radicals [43]. As the major carotenoid in the pulp of 'Tommy Atkins' mangoes [44], β-carotene was quantified in our study to determine changes in fruit ripening.…”

Modified Atmospheric Packaging of Fresh-Cut Amaranth (Amaranthus tricolor L.) for Extending Shelf Life

“…As shown in Figure 7B, the MAP groups successfully curtailed the rapid increase in MDA content with respect to the CK group (p < 0.05). Chen et al [44] also found that MAP inhibited chlorophyll degradation and chloroplast destruction, thereby maintaining the green color of lettuce, by blocking MDA production. ROS induction plays a major role in enhancing MDA content [45].…”

Effective pretreatment technologies for fresh foods aimed for use in central kitchen processing

“…The proportions of MAP gases for selected products are listed in Table 5. 145,146,148,150,[153][154][155][156][157][158][159][160] It can be seen that different foods require different gas compositions, and N 2 , O 2 , and CO 2 are the most commonly used gases for MAP.…”

“…MAP resulted in a longer shelf life; quality and safety of the fillets were well maintained. The proportions of MAP gases for selected products are listed in Table 5 145,146,148,150,153–160 . It can be seen that different foods require different gas compositions, and N 2 , O 2 , and CO 2 are the most commonly used gases for MAP.…”