Ivory vs. osseous ivory substitutes—Non-invasive diffractometric discrimination

Hoelzig, Hieronymus; Muenster, Tom; Blanke, S.; Kloess, Gert; Garmasukis, R.; Köenig, Andreas

doi:10.1016/j.forsciint.2020.110159

Cited by 3 publications

(3 citation statements)

References 43 publications

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“…In contrast, the En-BVs group exhibited better crystallinity and showed diffraction rings on the (002) and (211) crystal faces (Figure b 3 ). The development of (310) and (002) crystal planes (corresponding to perpendicular and parallel crystal C -axis orientations, respectively) can indicate the orientation of apatite crystals …”

Section: Resultsmentioning

confidence: 99%

“…The development of (310) and (002) crystal planes (corresponding to perpendicular and parallel crystal C-axis orientations, respectively) can indicate the orientation of apatite crystals. 53 The development of crystalline facets has been influenced by the concentrations of Ca 2+ and Pi within the confined microcompartment. The concentration of autogenously released Pi catalyzed by activated-ALP within the mineralization microcompartments of the Ad-BVs on day 1 was 0.93 ± 0.08 mmol L −1 , approximately equivalent to the phosphate level in human body fluids (Figure 2g).…”

Section: Crystallization During the Earlymentioning

confidence: 99%

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Enzyme-Activated Biomimetic Vesicles Confining Mineralization for Bone Maturation

Chen,

Zhao,

Tang

et al. 2024

ACS Appl. Mater. Interfaces

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Inspired by the crucial role of matrix vesicles (MVs), a series of biomimetic vesicles (BVs) fabricated by calcium glycerophosphate (CaGP) modified polyurethane were designed to mediate the mineralization through in situ enzyme activation for bone therapy. In this study, alkaline phosphatase (ALP) was harbored in the porous BVs by adsorption (Ad-BVs) or entrapment (En-BVs). High encapsulation of ALP on En-BVs was effectively self-activating by calcium ions of CaGP-modified PU that specifically hydrolyzed the organophosphorus (CaGP) to inorganic phosphate, thus promoting the formation of the highly oriented bone-like apatite in vitro. Enzyme-catalyzed kinetics confirms the regulation of apatite crystallization by the synergistic action of self-activated ALP and the confined microcompartments of BVs. This leads to a supersaturated microenvironment, with the En-BVs group exhibiting inorganic phosphate (Pi) levels 4.19 times higher and Ca 2+ levels 3.67 times higher than those of simulated body fluid (SBF). Of note, the En-BVs group exhibited excellent osteo-inducing differentiation of BMSCs in vitro and the highest maturity with reduced bone loss in rat femoral defect in vivo. This innovative strategy of biomimetic vesicles is expected to provide valuable insights into the enzyme-activated field of bone therapy.

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Section: Resultsmentioning

confidence: 99%

Section: Crystallization During the Earlymentioning

confidence: 99%

Enzyme-Activated Biomimetic Vesicles Confining Mineralization for Bone Maturation

Chen,

Zhao,

Tang

et al. 2024

ACS Appl. Mater. Interfaces

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“…Nondestructive methods such as x-ray diffractometry have been used to discriminate between mineralized tissues, such as bone, antler, and ivory ( 19 ), whereas small and wide-angle x-ray scattering, cross-polarized light microscopy, and scanning electron microscopy have all been used to study orientation and three-dimensional (3D) arrangement of the mineralized collagen fibers in ivory that relate to the macroscopic Schreger pattern ( 20 , 21 ). In addition to this, synchrotron radiation micro–computed tomography (SR-μCT) scanning has been used to characterize the 3D morphology of ivory, antler, land mammal, and whale bones at a high resolution to differentiate them ( 22 ).…”

Section: Introductionmentioning

confidence: 99%

Species identification of ivory and bone museum objects using minimally invasive proteomics

Gilbert,

Krupicka,

Galluzzi

et al. 2024

Sci. Adv.

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Ivory is a highly prized material in many cultures since it can be carved into intricate designs and have a highly polished surface. Due to its popularity, the animals from which ivory can be sourced are under threat of extinction. Identification of ivory species is not only important for CITES compliance, it can also provide information about the context in which a work was created. Here, we have developed a minimally invasive workflow to remove minimal amounts of material from precious objects and, using high-resolution mass spectrometry–based proteomics, identified the taxonomy of ivory and bone objects from The Metropolitan Museum of Art collection dating from as early as 4000 B.C. We built a proteomic database of underrepresented species based on exemplars from the American Museum of Natural History, and proposed alternative data analysis workflows for samples containing inconsistently preserved organic material. This application demonstrates extensive ivory species identification using proteomics to unlock sequence uncertainties, e.g., Leu/Ile discrimination.

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Investigating the utility of a handheld X-ray fluorescence (XRF) device as a field test for suspected ivory

McLellan

Carrothers

Spicer

2022

Forensic Science International: Animals and Environments

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Ivory vs. osseous ivory substitutes—Non-invasive diffractometric discrimination

Cited by 3 publications

References 43 publications

Enzyme-Activated Biomimetic Vesicles Confining Mineralization for Bone Maturation

Enzyme-Activated Biomimetic Vesicles Confining Mineralization for Bone Maturation

Species identification of ivory and bone museum objects using minimally invasive proteomics

Investigating the utility of a handheld X-ray fluorescence (XRF) device as a field test for suspected ivory

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