J. H. Badley and Bedales

Stewart, W. A. C.

doi:10.1007/978-1-349-00120-0_14

Cited by 3 publications

(5 citation statements)

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“…In the present study, we show, for the first time, the generation of mitochondrial ROS in HT-29 cells exposed to transresveratrol for 4 h. This time was chosen since ROS production in mitochondria has been characterized as an early pro-apoptotic event that takes place before the commitment of the cells to undergo apoptosis (23). Our results are in agreement with previous studies that pointed out superoxide anions as secondary messengers in apoptosis provoked by anticancer agents, such as paclitaxel and cisplatin (13,14). Our findings are also consistent with a study that indicated that treatment of prostate cancer cells with trans-resveratrol resulted in the generation of reactive oxygen species, prior to the release of mitochondrial proteins to the cytosol, activation of effector caspase-3, and caspase-9, and induction of apoptosis (11).…”

Section: Discussionsupporting

confidence: 90%

“…Despite the numerous studies describing the intracellular changes leading to cell cycle arrest or apoptosis in response to trans-resveratrol treatment, the effects are often cell type specific, and the underlying mechanism of action remains to be fully identified (4). Several studies have indicated that reactive oxygen species (ROS) production in mitochondria may mediate apoptosis induction (13,14). The roles of ROS and mitochondria in trans-resveratrol induced apoptosis are still undefined.…”

Section: Introductionmentioning

confidence: 99%

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Resveratrol Induces Apoptosis through ROS-Dependent Mitochondria Pathway in HT-29 Human Colorectal Carcinoma Cells

Juan

Wenzel

Daniel

et al. 2008

J. Agric. Food Chem.

182

114

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trans-Resveratrol is a polyphenol found in blueberries, grapes, and wine with cancer chemopreventive properties. The low bioavailability of this compound enhances its concentration in the luminal content and becomes a potential chemopreventive agent against colon cancer. In the present study, the antiproliferative and pro-apoptotic effects on the human colorectal carcinoma HT-29 cells as well as the mechanisms underlying these effects were examined. Proliferation, cytotoxicity, and apoptosis were measured by fluorescence-based techniques. Studies of dose-dependent effects of trans-resveratrol showed antiproliferative activity with an EC 50 value of 78.9 +/- 5.4 microM. Caspase-3 was activated in a dose-dependent manner after incubation for 24 h giving an EC 50 value of 276.1 +/- 1.7 microM. Apoptosis was also confirmed with microscopic observation of changes in membrane permeability and detection of DNA fragmentation. The activity of trans-resveratrol on the mitochondria apoptosis pathway was evidenced by the production of superoxide anions in the mitochondria of cells undergoing apoptosis. In conclusion, trans-resveratrol inhibits cell proliferation without cytotoxicity and induces apoptosis in HT-29. Results of the present study provide evidence demonstrating the antitumor effect of trans-resveratrol via a ROS-dependent apoptosis pathway in colorectal carcinoma.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Resveratrol Induces Apoptosis through ROS-Dependent Mitochondria Pathway in HT-29 Human Colorectal Carcinoma Cells

Juan

Wenzel

Daniel

et al. 2008

J. Agric. Food Chem.

182

114

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“…The mRNA were about 1 kb in length and possessed a 585-base open reading frame that coded for polypeptides 195 amino acids in length. Each polypeptide was predicted to contain a 23-residue signal sequence, with residue 1 of the mature protein being a cysteine, a prediction confirmed by amino terminal amino acid sequencing (15)(16)(17). Surprisingly.…”

Section: Identification Of Otp-1 and Btp-1 As Interferonsmentioning

confidence: 93%

A role for interferons in early pregnancy

Roberts

1991

BioEssays

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In order to survive, the developing conceptus must interrupt the normal ovarian cycle of the mother and extend the production of progesterone by the corpus luteum. An unusual Type 1 interferon (IFN), related structurally to the IFN-alpha molecule and produced in massive amounts for only a few days by the first epithelium (trophectoderm) of the preimplantation conceptus, has been implicated as the antiluteolytic agent in sheep and cattle. IFN-alpha therapy during this critical period can also improve pregnancy success in sheep. It remains unclear, however, whether the trophoblast IFN have specialized biological properties or whether they are unique merely in the timing, magnitude and site of their expression.

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“…above, 3.0 g) is swelled in toluene [12] (25 mI). tert.Butyloxycarbonyl-glycine p-nitrophenyl ester [13] (3.55 gm, 12 mmoI) and imidazole [11] (1.2 g about 18 mmoI) are added and the suspension is stirred at room temperature overnight. The resin is thoroughly [9] washed on a filter with toluene and then with dichloromethane and dried in air.…”

Section: L-glutamic Acid «-Benzyl Ester [1] (mentioning

confidence: 99%

“…Liquid isobutylene (30 ml) is slowly added to L-tyrosine (3.62 g, 20 mmol) dissolved in a mixture of dioxane (30 ml) and p-toluenesulfonic acid (monohydrate [10], 7.6 g, 40 mmol) in a 500 ml pressure bottle [11]. The flask is securely stoppered, wrapped in a towel and shaken at room temperature for about 20 hours.…”

Section: Esterification Of Free Amino Acids Through Acid Catalyzedmentioning

confidence: 99%