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Abstract

Background: Leptadenia reticulata (Wight & Arn.) has made important contributions to both traditional and modern medicine. Aims and Objectives: The study aims to develop and validate an high-performance thinlayer chromatography (HPTLC) method for the determination of quercetin content in L. reticulata collected from various parts of the country and to assess its anti-inflammatory activity using the bovine serum albumin (BSA) inhibition method. Materials and Methods: Five samples were obtained from different geographical locations in India, and no significant variation was found in the morpho-anatomical study. HPTLC method developed using a toluene: chloroform: ethyl acetate: formic acid (2.5: 2: 4.5: 1) mobile phase at a wavelength of 300 nm. Second, anti-inflammatory activity of L. reticulata extract was assessed using the BSA inhibition approach. Results: For the standard and sample, R f value of quercetin (0.49) was observed. Concentration range of quercetin (60-160 ng/spot) was determined to be linear for the linearity approach, with a regression coefficient for quercetin of 0.999 and an optimal recovery of 101.70%. The limits of detection and limits of quantification were found to be 0.78 and 2.36 ng, respectively. Study revealed quercetin content differed among L. reticulata collected from different geographical regions. The highest and lowest quercetin contents in L. reticulata were detected in LR-103 and LR-099, respectively. From the results obtained, sample LR-103 showed effective anti-inflammatory activity compared to LR-099 at certain concentrations. Conclusion: The developed method is suitable for the detection and quantification of the lowest content of quercetin in L. reticulata. The obtained data indicate that geographic origin and climatic conditions may be major factors in determining the phytocompounds and biological activities of L. reticulata plant species.