Jaagsiekte sheep retrovirus is not detected in human lung adenocarcinomas expressing antigens related to the Gag polyprotein of betaretroviruses

Heras, M. De las; Ortı́n, A.; Azúa, J.; Borderías, Luis; Álvarez, Ramiro; Jiménez-Más, José Antonio; Marchetti, Antonio; Palmarini, Massimo

doi:10.1016/j.canlet.2007.08.006

Cited by 14 publications

(19 citation statements)

References 35 publications

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“…However, there is no clear consensus on the correlation between JSRV and human lung cancer (Yousem et al, 2001;De Las Heras et al, 2007;Hopwood et al, 2010). Interestingly, one study has reported that a subset of human lung cancers expressed an antigen that could react with a JSRV Envspecific monoclonal antibody.…”

Section: Discussionmentioning

confidence: 99%

Detection of Jaagsiekte sheep retrovirus in the peripheral blood during the pre-clinical period of ovine pulmonary adenomatosis

Liu¹,

Zhang²,

Sun³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. The envelope protein (Env) of the Jaagsiekte sheep retrovirus (JSRV) is known to be a unique oncoprotein responsible for inducing ovine pulmonary adenocarcinoma (OPA). The objective of this study was to prepare a specific monoclonal antibody (mAb) against the JSRV Env protein using bioinformatic analysis. According to the structure and epitope prediction results of JSRV Env, the JSRV-Env 572-615 antigen was prepared via peptide synthesis (amino acid sequence 572-615, denoted as JSRV-Env 572-615 ). BALB/c mice were immunized to prepare the anti-JSRV-Env 572-615 mAb. Spleen cells were fused with SP2/0 myeloma cells after being screened by indirect ELISA and cloned by limiting dilution. The specificity of mAb was evaluated by western blot analysis and immunohistochemistry assays. Western blot results showed that the JSRV Env protein was able to bind to mAb with high specificity. Immunohistochemistry assays demonstrated that the mAb was able to recognize JSRV Env in adenomatous hyperplasia of the lung. Furthermore, JSRV was detected in peripheral blood leukocytes during the pre-clinical period of OPA in 2 of the 25 sheep using this newly synthesized mAb. Therefore, this mAb may be a useful tool for the detection of JSRV in sheep.

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Section: Discussionmentioning

confidence: 99%

Detection of Jaagsiekte sheep retrovirus in the peripheral blood during the pre-clinical period of ovine pulmonary adenomatosis

Liu¹,

Zhang²,

Sun³

et al. 2016

Genet. Mol. Res.

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“…A retroviral etiology for these human tumors has been suggested, and some cases have been shown to express an antigen related to betaretroviral Gag proteins (20,22,38). However, additional markers of retroviral infection in these patients have not been found (38).…”

mentioning

confidence: 99%

Jaagsiekte Sheep Retrovirus Infects Multiple Cell Types in the Ovine Lung

Martineau

Cousens

Imlach

et al. 2011

J Virol

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“…These Env proteins might reveal other cellular pathways involved in oncogenic transformation. In addition, the possible involvement of unknown or known retroviruses in human cancers should be explored [125][126][127][128][129][130][153][154][155][156][157]. In the future, identification of novel oncogenic retroviruses or implication of known retroviruses as the causative agents of human cancers would be exciting and important.…”

Section: Resultsmentioning

confidence: 99%

“…Interestingly, some hLAC tissue samples cross-react with JSRV Gag and human endogenous retrovirus-K (HERV-K) Gag antisera, but not with JSRV SU antiserum [125,126], suggesting that hLACs may express betaretrovirus-like antigens although they are not infected by JSRV itself. Two reports supported the presence of JSRV-related sequences in individuals by PCR-based analysis [127,128], but two reports did not [129,130].…”

Section: Jaagsiekte Sheep Retrovirus (Jsrv)mentioning

confidence: 97%

Oncogenesis by retroviruses: old and new paradigms

Maeda

Fan

Yoshikai

2008

Reviews in Medical Virology

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Retroviruses are associated with a variety of diseases including an array of malignancies, immunodeficiencies and neurological disorders. In particular, studies of oncogenic retroviruses established fundamental principles of modern molecular cancer biology. Studies of avian Rous sarcoma virus (RSV) led to the discovery of the viral oncogene src, and this was followed by the discovery of other viral oncogenes in retroviruses of mammals including rodents, cats, monkeys and so forth. Studies of the viral oncogenes in turn led to the discovery of cellular proto-oncogenes in the host genome; cellular oncogenes have been shown to be activated in a variety of human cancers, including those with no viral involvement. Oncogenic animal retroviruses can be divided into two groups based on their mechanisms of tumourigenesis, acute transforming retroviruses and nonacute retroviruses. Acute transforming retroviruses are typically replication defective and they induce tumours rapidly due to expression of their viral oncogenes. Nonacute retroviruses are replication competent and they induce tumours with longer latencies, by activating cellular proto-oncogenes in the tumour cells; this results from insertion of proviral DNA in the vicinity of the activated proto-oncogene. More recently, human T-cell leukaemia virus type I (HTLV-I) was discovered as an etiological agent of human cancer (adult T-cell leukaemia [ATL]); this virus also encodes regulatory genes some of which are important for its oncogenic potential. Most recently, the retroviral structural protein Envelope (Env) has been shown to be directly involved in oncogenic transformation for certain retroviruses. Env-induced transformation is a new paradigm for retroviral oncogenesis. In this review, we will summarise research on retrovirus oncogenic transformation over the past 100 years since the first published report of an oncogenic virus with particular attention to Env-induced transformation.

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Jaagsiekte sheep retrovirus is not detected in human lung adenocarcinomas expressing antigens related to the Gag polyprotein of betaretroviruses

Cited by 14 publications

References 35 publications

Detection of Jaagsiekte sheep retrovirus in the peripheral blood during the pre-clinical period of ovine pulmonary adenomatosis

Detection of Jaagsiekte sheep retrovirus in the peripheral blood during the pre-clinical period of ovine pulmonary adenomatosis

Jaagsiekte Sheep Retrovirus Infects Multiple Cell Types in the Ovine Lung

Oncogenesis by retroviruses: old and new paradigms

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