Jasmonic acid and ethylene signaling pathways participate in the defense response of Chinese cabbage to Pectobacterium carotovorum infection

Chen, Chang-long; Yuan, Fang; Li, Xiaoying; Ma, Ruijuan; Xie, Hua

doi:10.1016/s2095-3119(20)63267-1

Cited by 22 publications

(9 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Pectobacterium strains were cultured overnight, and a pellet was collected by centrifugation at 8,000 rpm and 4°C for 1 min. Total RNA was extracted from the cells using the Spin Column Bacteria Total RNA Purification Kit (Sangon Biotech, China) and treated with DNase I. cDNA was then synthesized from the total RNA and subjected to qPCR using the TB Green Premix Ex Taq II (Tli RNaseH Plus) (TaKaRa, China), as previously described ( 44 ). RecA was used as the qPCR reference gene.…”

Section: Methodsmentioning

confidence: 99%

A genome-wide association study reveals that epistasis underlies the pathogenicity of Pectobacterium

Chen,

Che,

Dong

et al. 2023

Microbiol Spectr

Self Cite

View full text Add to dashboard Cite

Pectobacterium spp. are important bacterial pathogens that cause soft rot symptoms in various crops. However, their mechanism of pathogenicity requires clarity to help control their infections. Here, genome-wide association studies (GWAS) were conducted by integrating genomic data and measurements of two phenotypes (virulence and cellulase activity) for 120 various Pectobacterium strains in order to identify the genetic basis of their pathogenicity. An artificial intelligence-based software program was developed to automatically measure lesion areas on Chinese cabbage, thereby facilitating accurate and rapid data collection for virulence phenotypes for use in GWAS analysis. The analysis discovered 428 and 158 loci significantly associated with Pectobacterium virulence (lesion area) and cellulase activity, respectively. In addition, 1,229 and 586 epistasis loci pairs were identified for the virulence and cellulase activity phenotypes, respectively. Among them, the AraC transcriptional regulator exerted epistasis effects with another three nutrient transport-related genes in pairs contributing to the virulence phenotype, and their epistatic effects were experimentally confirmed for one pair with knockout mutants of each single gene and double gene. This study consequently provides valuable insights into the genetic mechanism underlying Pectobacterium spp. pathogenicity. IMPORTANCE Plant diseases and pests are responsible for the loss of up to 40% of food crops, and annual economic losses caused by plant diseases reach more than $220 billion. Fighting against plant diseases requires an understanding of the pathogenic mechanisms of pathogens. This study adopted an advanced approach using population genomics integrated with virulence-related phenotype data to investigate the genetic basis of Pectobacterium spp., which causes serious crop losses worldwide. An automated software program based on artificial intelligence was developed to measure the virulence phenotype (lesion area), which greatly facilitated this research. The analysis predicted key genomic loci that were highly associated with virulence phenotypes, exhibited epistasis effects, and were further confirmed as critical for virulence with mutant gene deletion experiments. The present study provides new insights into the genetic determinants associated with Pectobacterium pathogenicity and provides a valuable new software resource that can be adapted to improve plant infection measurements.

show abstract

Section: Methodsmentioning

confidence: 99%

A genome-wide association study reveals that epistasis underlies the pathogenicity of Pectobacterium

Chen,

Che,

Dong

et al. 2023

Microbiol Spectr

Self Cite

View full text Add to dashboard Cite

show abstract

“…Twenty DEGs related to cell motility were selected for validation by means of qPCR from the transcriptome data of Po ( Zur ) vs. Po (EV). The cDNA was prepared from the remaining total RNA after transcriptome sequencing by EasyScript One-step gDNA removal and cDNA synthesis supermix (TransGen Biotech, Beijing, China) and subjected to qPCR using TB Green ® Premix Ex Taq™ II (Tli RNaseH Plus) (TaKaRa, China) as previously described [ 51 ]. RecA was used as the internal reference gene.…”

Section: Methodsmentioning

confidence: 99%

Investigation of the Role of a Zinc Uptake Regulator (Zur) in the Virulence of Pectobacterium odoriferum

Chen

Cui

Guan

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

Pectobacterium spp. infect many horticultural crops worldwide and lead to serious crop losses. Zinc-uptake-regulator (Zur) proteins are present widely in prokaryotes and play an important role in pathogenicity. To uncover the role of Zur in P. odoriferum, we constructed mutant (ΔZur) and overexpression [Po (Zur)] strains of a Zur, and a virulence assay showed that the Po (Zur) was of significantly lower virulence, while the ΔZur displayed significantly increased virulence on Chinese cabbage compared to their respective control strains, wild-type P. odoriferum (Po WT) and P. odoriferum harboring an empty vector (Po (EV)) (p < 0.05). The growth curves of the ΔZur and Po (Zur) showed no obvious differences from those of the control strains. Comparative transcriptome analysis showed that Zur overexpression in P. odoriferum induced differentially expressed genes (DEGs) related to flagellum and cell motility, while mutating Zur resulted in DEGs mainly corresponding to divalent-metal-ion transport and membrane transport. Phenotypic experiments on the Po (Zur) showed that flagellum numbers and cell motility were reduced in comparison with the control, while those of the ΔZur did not change. Collectively, these results show that the Zur negatively regulates the virulence of P. odoriferum and might function via a dual mechanism dependent on dose.

show abstract

“…However, such a response would be inappropriate for nonpathogenic bacteria or microorganisms that have not yet breached plant cell walls [42]. Therefore, to combat necrotrophic pathogens such as Pectobacterium carotovorum [43] (which obtain their resources from disrupted or dying plant cells), plants activate jasmonic acid (JA)-dependent pathways, resulting in the accumulation of phenolic compounds, defensins, and cell wall strengthening to suppress the necrotrophic pathogen attacks [44]. Generally, JA-and SA-dependent pathways are antagonistic toward each other, although the exact interactions between those pathways are yet unknown [45].…”

Section: Induced Resistancementioning

confidence: 99%

“…Throughout their evolution, plants have developed specific receptors, such as pattern recognition receptors (PRRs), that enable them to recognize various types of threats. These threats include the recognition of herbivore-associated molecular patterns (HAMPs) from herbivores, pathogen-associated molecular patterns (PAMPs) from pathogens, and microbe-associated molecular patterns (MAMPs) from other microorganisms; however, they combat necrotrophic pathogens such as Pectobacterium carotovorum [43] (which obtain their resources from disrupted or dying plant cells), plants activate jasmonic acid (JA)-dependent pathways, resulting in the accumulation of phenolic compounds, defensins, and cell wall strengthening to suppress the necrotrophic pathogen attacks [44]. Generally, JA-and SA-dependent pathways are antagonistic toward each other, although the exact interactions between those pathways are yet unknown [45].…”

Section: Induced Resistancementioning

confidence: 99%

Microbial Consortia for Plant Protection against Diseases: More than the Sum of Its Parts

Maciag,

Kozieł,

Rusin

et al. 2023

IJMS

View full text Add to dashboard Cite

Biological plant protection presents a promising and exciting alternative to chemical methods for safeguarding plants against the increasing threats posed by plant diseases. This approach revolves around the utilization of biological control agents (BCAs) to suppress the activity of significant plant pathogens. Microbial BCAs have the potential to effectively manage crop disease development by interacting with pathogens or plant hosts, thereby increasing their resistance. However, the current efficacy of biological methods remains unsatisfactory, creating new research opportunities for sustainable plant cultivation management. In this context, microbial consortia, comprising multiple microorganisms with diverse mechanisms of action, hold promise in terms of augmenting the magnitude and stability of the overall antipathogen effect. Despite scientific efforts to identify or construct microbial consortia that can aid in safeguarding vital crops, only a limited number of microbial consortia-based biocontrol formulations are currently available. Therefore, this article aims to present a complex analysis of the microbial consortia-based biocontrol status and explore potential future directions for biological plant protection research with new technological advancements.

show abstract

Jasmonic acid and ethylene signaling pathways participate in the defense response of Chinese cabbage to Pectobacterium carotovorum infection

Cited by 22 publications

References 34 publications

A genome-wide association study reveals that epistasis underlies the pathogenicity of Pectobacterium

A genome-wide association study reveals that epistasis underlies the pathogenicity of Pectobacterium

Investigation of the Role of a Zinc Uptake Regulator (Zur) in the Virulence of Pectobacterium odoriferum

Microbial Consortia for Plant Protection against Diseases: More than the Sum of Its Parts

Contact Info

Product

Resources

About