Jasmonic Acid-Involved OsEDS1 Signaling in Rice-Bacteria Interactions

Abstract

Background The function of Arabidopsis enhanced disease susceptibility 1 (AtEDS1) and its sequence homologs in other dicots have been extensively studied. However, it is unknown whether rice EDS1 homolog (OsEDS1) plays a role in regulating the rice-pathogen interaction. Results In this study, a OsEDS1 -knouckout mutant ( oseds1 ) was characterized and shown to have increased susceptibility to Xanthomonas oryzae pv. oryzae ( Xoo ) and Xanthomonas oryzae pv. oryzicola ( Xoc ), suggesting the positive role of OsEDS1 in regulating rice disease resistance. However, the following evidence suggests that OsEDS1 shares some differences with AtEDS1 in its way to regulate the host-pathogen interactions. Firstly, OsEDS1 modulates the rice-bacteria interactions involving in jasmonic acid (JA) signaling pathway, while AtEDS1 regulates Arabidopsis disease resistance against biotrophic pathogens depending on salicylic acid (SA) signaling pathway. Secondly, introducing AtEDS1 could reduce oseds1 mutant susceptibility to Xoo rather than to Xoc . Thirdly, exogenous application of JA and SA cannot complement the susceptible phenotype of the oseds1 mutant, while exogenous application of SA is capable of complementing the susceptible phenotype of the ateds1 mutant. Finally, OsEDS1 is not required for R gene mediated resistance, while AtEDS1 is required for disease resistance mediated by TIR-NB-LRR class of R proteins. Conclusion OsEDS1 is a positive regulator in rice-pathogen interactions, and shares both similarities and differences with AtEDS1 in its way to regulate plant-pathogen interactions. Electronic supplementary material The online version of this article (10.1186/s12284-019-0283-0) contains supplementary material, which is available to authorized users.

Deciphering Core-Phyllomicrobiome of Rice Genotypes Grown in Contrasting Mountain and Island Agroclimatic Zones: Implications for Microbiome Engineering Against Blast Disease

“…OsPAD4 and OsEDS1 play an important role in jasmonic acid-mediated induced systemic resistance. The accumulation of rice phytoalexin mamilactone-A is reported to be modulated by the expression of the OsPAD4 gene and is known to govern blast resistance [48,49,98]. Marginal induction of OsNPR1, OsFMO, OsPDF2.2, and OsPR1.1 was observed in bacterized seedlings.…”

“…Candidate defense genes: Putative defense genes, OsCEBiP [46], OsCERK1 [47], OsPAD4 [48], OsEDS1 [49], OsNPR1 [50], OsPDF2.2 [51], OsFMO1 [52,53] and OsPR1.1 [54] were chosen; PCR primers targeting the above defense genes are presented (Supplementary Table 1-2). The qPCR experiment was conducted in a Real-Time PCR instrument (Light Cycler 96, Roche Life Science, Switzerland) using GoTaq® 1-Step RT-qPCR System; qPCR reaction conditions were as follows; one cycle of reverse transcription at 37 °C for 15 minutes followed by reverse-transcriptase inactivation step of 95 °C for 10 minutes followed by 30 cycles of 95 °C for 10 seconds, annealing at 58 °C for 30 seconds and extension at 72 °C for 30 sec followed by three-step melting of 95°C for 10 seconds, 63°C for 60 seconds and 97 °C for 1 second and then nal cooling at 37 °C for 30 seconds.…”

Overexpression of OsPUB41, a Rice E3 ubiquitin ligase induced by cell wall degrading enzymes, enhances immune responses in Rice and Arabidopsis

“…However, role of OsEDS1, rice orthologue of AtEDS1, in plant (rice) defense depends on JA signaling. Expression of AtEDS1 in oseds1 (OsEDS1 knockout rice plants) partially complements oseds1 defense phenotype [51]. Apart from known commonalities, there exist quite a few subtle differences in the regulation and execution of defense responses in rice and Arabidopsis (monocots and dicots).…”

Deciphering Core-Phyllomicrobiome of Rice Genotypes Grown In Contrastingmountain And Island Agroclimatic Zones: Implications For Phyllomicrobiome Engineering Against Blast Disease

“…Candidate defense genes: Putative defense genes, OsCEBiP [46], OsCERK1 [47], OsPAD4 [48], OsEDS1 [49], OsNPR1 [50], OsPDF2.2 [51], OsFMO1 [52,53]and OsPR1.1 [54] were chosen; PCR primers targeting the above defense genes are presented (Supplementary Table 1-2). The qPCR experiment was conducted in a Real-Time PCR instrument (Light Cycler 96, Roche Life Science, Switzerland) using GoTaq® 1-Step RT-qPCR System; qPCR reaction conditions were as follows; one cycle of reverse transcription at 37 °C for 15 minutes followed by reversetranscriptase inactivation step of 95 °C for 10 minutes followed by 30 cycles of 95 °C for 10 seconds, annealing at 58 °C for 30 seconds and extension at 72 °C for 30 sec followed by three-step melting of 95°C for 10 seconds, 63 °C for 60 seconds and 97 °C for 1 second and then nal cooling at 37 °C for 30 seconds.…”

“…OsPAD4 and OsEDS1 play an important role in jasmonic acidmediated induced systemic resistance. The accumulation of rice phytoalexin mamilactone-A is reported to be modulated by the expression of the OsPAD4 gene and is known to govern blast resistance[48,49, 98]. Marginal induction of OsNPR1, OsFMO, OsPDF2.2, and OsPR1.1was observed in bacterized seedlings.…”