Jet aeration as alternative to overcome mass transfer limitation of stirred bioreactors

Weber, Sebastian; Schaepe, Sebastian; Freyer, Stephan; Kopf, Michael-Helmut; Dietzsch, Christian

doi:10.1002/elsc.201700169

Cited by 13 publications

(9 citation statements)

References 27 publications

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“…Typical P/V values of JLR ranged from 50 to 5200 W m −3 with Q L values varying from 2 to 70 L min −1 17,20,30‐32 . Several authors have claimed lower P/V values of the JLR compared to the STR 28,33 …”

Section: Resultsmentioning

confidence: 99%

Intensification of multiphase systems with a jet‐loop reactor: characterization of mass transfer, mixing time, drop oil size, and biomass growth

Bourguett‐Rosas,

Arenas‐García,

Vergara‐Porras

et al. 2024

J of Chemical Tech & Biotech

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BACKGROUNDThe development of processes for transforming vegetable oils into useful products has received increasing attention in the biotechnological field. The reactor used for this purpose should meet adequate mass transfer, mixing and oil dispersion conditions. In this work comprehensive research into the use of a jet‐loop reactor as a potential alternative to airlift and stirred tank reactors was conducted in presence of avocado oil in a water‐air system. Additionally, the assessment of the jet‐loop for biomass growth in presence of oil was performed.RESULTSThe increase of avocado oil from 1 to 5 % v/v resulted in a reduction in the performance of the three reactors. Despite the airlift reactor having the lowest power input, it exhibited the worst performance in terms of mass transfer, mixing and oil dispersion. The estimation of the energetic oxygen transfer efficiency (Kg O2 KW‐1 h‐1) allowed the determination that the jet‐loop reactor was superior to the stirred tank. This superiority was attributed to the low values of the mixing time and oil droplet diameter observed in the jet‐loop reactor. Finally, all these parameters had a positive impact on the growth of Wautersia eutropha in the jet‐loop reactor compared with the other two reactors.CONCLUSIONSThe results showed that the jet‐loop reactor presented superior performance in most cases compared to the stirred tank and airlift reactors. Particularly, the jet‐loop exhibits an interesting potential to reduce the size of the avocado oil droplets, which could lead to a significative increase in the growth rate of Wautersia eutropha.This article is protected by copyright. All rights reserved.

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Section: Resultsmentioning

confidence: 99%

Intensification of multiphase systems with a jet‐loop reactor: characterization of mass transfer, mixing time, drop oil size, and biomass growth

Bourguett‐Rosas,

Arenas‐García,

Vergara‐Porras

et al. 2024

J of Chemical Tech & Biotech

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“…Subsequently, the aeration was started and the increasing oxygen concentration (c O2 ) was measured by an optical oxygen sensor. The k L a values were determined from the respective step response between 20 and 80% of the maximum dissolved oxygen concentration (c O2 * ) (Equation ).

{normalk}_{normalL} normala = \frac{ln (\frac{{normalc}_{O 2}^{*} - {normalc}_{O 2, 1}}{{normalc}_{O 2}^{*} - {normalc}_{O 2, 2}})}{{normalt}_{2} - {normalt}_{1}}

…”

Section: Methodsmentioning

confidence: 99%

“…In recent studies, an increase in mass transfer up to ∼200% and therefore higher productivities were achieved in jet loop reactors (JLRs) compared to stirred tank reactors (STRs) . Surprisingly in some cases the concentration of the anaerobic and overflow metabolite acetate was already increasing for cultivation at strictly aerobic conditions . Therefore, the presence of oxygen‐ and substrate gradients within the JLR was considered as a possible reason for the observed acetate formation.…”

Section: Introductionmentioning

confidence: 99%

Monitoring gradient formation in a jet aerated bioreactor

Weber

Schaepe

Freyer

et al. 2018

Engineering in Life Sciences

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Jet aerated loop reactors (JLRs) provide high mass transfer coefficients (kLa) and can be used for the intensification of mass transfer limited reactions. The jet loop reactor achieves higher kLa values than a stirred tank reactor (STR). The improvement relies on significantly higher local power inputs (∼104) than those obtainable with the STR. Operation at high local turnover rates requires efficient macromixing, otherwise reactor inhomogeneities might occur. If sufficient homogenization is not achieved, the selectivity of the reaction and the respective yields are decreased. Therefore, the balance between mixing and mass transfer in jet loop reactors is a critical design aspect. Monitoring the dissolved oxygen levels during the turnover of a steady sodium sulfite feed implied the abundance of gradients in the JLR. Prolonged mixing times at identical power input and aeration rates (∼100%) were identified for the JLR in comparison to the STR. The insertion of a draft tube to the JLR led to a more homogenous dissolved oxygen distribution, but unfortunately a reduction of mixing time was not achieved. In case of increased medium viscosities as they may arise in high cell density cultivations, no gradient formation was detected. However, differences in medium viscosity significantly altered the mass transfer and mixing performance of the JLR.

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“…As a consequence, an increased gas liquid interface leads to reasonably higher k L a values per power input and therefore also to more efficient mass transfer [20]. As a result, a higher productivity was achieved [21]. As a result, a higher productivity was achieved [21].…”

Section: Alternative Reactors For Aerobic Bioprocessesmentioning

confidence: 99%

Impact of nozzle operation on mass transfer in jet aerated loop reactors. Characterization and comparison to an aerated stirred tank reactor

Weber

Schaepe

Freyer

et al. 2018

Engineering in Life Sciences

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The impact of mass transfer on productivity can become a crucial aspect in the fermentative production of bulk chemicals. For highly aerobic bioprocesses the oxygen transfer rate (OTR) and productivity are coupled. The achievable space time yields can often be correlated to the mass transfer performance of the respective bioreactor. The oxygen mass transfer capability of a jet aerated loop reactor is discussed in terms of the volumetric oxygen mass transfer coefficient kLa [h−1] and the energetic oxygen transfer efficiency E [kgO2 kW−1 h−1]. The jet aerated loop reactor (JLR) is compared to the frequently deployed aerated stirred tank reactor. In jet aerated reactors high local power densities in the mixing zone allow higher mass transfer rates, compared to aerated stirred tank reactors. When both reactors are operated at identical volumetric power input and aeration rates, local kLa values up to 1.5 times higher are possible with the JLR. High dispersion efficiencies in the JLR can be maintained even if the nozzle is supplied with pressurized gas. For increased oxygen demands (above 120 mmol L−1 h−1) improved energetic oxygen transfer efficiencies of up to 100 % were found for a JLR compared to an aerated stirred tank reactor operating with Rushton turbines.

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Jet aeration as alternative to overcome mass transfer limitation of stirred bioreactors

Cited by 13 publications

References 27 publications

Intensification of multiphase systems with a jet‐loop reactor: characterization of mass transfer, mixing time, drop oil size, and biomass growth

Intensification of multiphase systems with a jet‐loop reactor: characterization of mass transfer, mixing time, drop oil size, and biomass growth

Monitoring gradient formation in a jet aerated bioreactor

Impact of nozzle operation on mass transfer in jet aerated loop reactors. Characterization and comparison to an aerated stirred tank reactor

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