Jiedu Sangen Decoction Inhibits the Invasion and Metastasis of Colorectal Cancer Cells by Regulating EMT through the Hippo Signaling Pathway

Abstract

Colorectal cancer (CRC) is one of the most common malignant tumors affecting the digestive tract. Moreover, the invasion and metastasis of CRC are the main reason therapy is usually inefficient. Decreased intercellular adhesion and enhanced cell motility induced by epithelial-mesenchymal transition (EMT) provide the basic conditions for the invasion and metastasis of the epithelial tumor cells of CRC. The Jiedu Sangen Decoction (JSD) is a prescription that has been used for more than 50 years in the treatment of CRC in the Zhejiang Hospital of Traditional Chinese Medicine. The aim of this study was to investigate the mechanism of JSD-triggered inhibition of invasion and metastasis in colon cancer. In vitro, the EMT model of the SW480 cells was induced by using epithelial growth factor (50 ng/mL). In vivo, the murine model of liver metastasis was constructed by inoculating mice with the SW480 cells. The effects of JSD on cell migration, invasion, and proliferation were determined using the transwell assay and CCK-8 assay. Moreover, the proteins related to the EMT process and the Hippo signaling pathway in the cancerous tissues and cell lines were determined by western blotting and immunostaining. JSD could significantly inhibit the proliferation, migration, and invasion of CRC cells and reverse their EMT status (all, P < 0.05). Moreover, after intervention with JSD, the levels of E-Cadherin (E-cad) increased, whereas the expression levels of N-Cadherin (N-cad), Yes-associated protein (YAP), and the transcriptional coactivator with the PDZ-binding motif (TAZ) decreased in both the SW480 cells and the tumor tissues. In summary, JSD reversed EMT and inhibited the invasion and metastasis of CRC cells through the Hippo signaling pathway.

Eva‐1 homolog A promotes papillary thyroid cancer progression and epithelial‐mesenchymal transition via the Hippo signalling pathway

“…Related proteins were detected to explore the mechanism of the EVA1A on EMT and apoptosis progression. Recent studies have provided enormous evidences suggesting that the Hippo signalling pathway can induce EMT 17,27,28 . Results revealed that compared with the control group, the Si‐ EVA1A group showed decreased N‐cadherin, vimentin and Bcl‐xL expression and increased Bax expression (Figure 6B).…”

<p>Traditional Chinese Medicine Xiaoai Jiedu Recipe Suppresses the Development of Hepatocellular Carcinoma via Regulating the microRNA-29a/Signal Transducer and Activator of Transcription 3 Axis</p>

“…6,7 For example, Jiedu Sangen Decoction, which has been used in the treatment of colorectal cancer (CRC) for over 50 years, has the ability to suppress the migration and invasion of CRC cells. 8,9 In addition, TCM has been long applied for the treatment of HCC. Besides, transcatheter arterial chemoembolization combined with JDF was shown to improve the prognosis of patients with HCC.…”

Canmei Formula Reduces Colitis-Associated Colorectal Carcinogenesis in Mice by Modulating the Composition of Gut Microbiota

“…Wing lam et al reported that PHY906 reduced the CPT-11-induced gastrointestinal toxicity by inhibiting multiple steps of inflammation and the promotion of intestinal progenitor cell repopulation (20). Extensive studies have revealed that TCM antitumor drugs work by inhibiting tumor proliferation, promoting tumor apoptosis, and affecting diverse molecular targets (21)(22)(23).…”