Jingfukang induces anti-cancer activity through oxidative stress-mediated DNA damage in circulating human lung cancer cells

Abstract

Background Metastasis is the main cause of lung cancer death. As a seed of metastasis, circulating tumor cells are an important target for metastasis intervention. The traditional Chinese medicine, Jinfukang, has been clinically available for the treatment of non-small cell lung cancer (NSCLC). In this study, we investigated the action and underlying mechanisms of Jinfukang against circulating lung tumor cells. Methods The cell counting kit-8 (CCK-8), colony formation and cell cycle assays were used to study the cell proliferation ability. Flow cytometry was used to detect the apoptosis and the expression level of ROS and Caspase-3. Comet and TUNEL assays were used to detect DNA damage. DNA damage related pathway protein was detected by western blot. Results Jinfukang significantly inhibits the proliferation of CTC-TJH-01 cells by inducing G1 phase arrest and inhibits their colony formation in a dose-dependent manner. Moreover, Jinfukang induces apoptosis in CTC-TJH-01 cells through the ROS-mediated ATM/ATR-p53 pathway and DNA damage. Conclusions Our findings suggest that Jinfukang may be a potential drug for lung cancer metastasis. Electronic supplementary material The online version of this article (10.1186/s12906-019-2601-x) contains supplementary material, which is available to authorized users.

Delicaflavone Represses Lung Cancer Growth by Activating Antitumor Immune Response through N6-Methyladenosine Transferases and Oxidative Stress

“…ROS might affect the prognosis of cancer patients through immune response and increase the sensitivity of cancer patients to chemotherapy [37]. The traditional Chinese medicine, Jinfukang, induces lung cancer cell apoptosis through the ROS-mediated ATM/ATR-p53 pathway and DNA damage [38]. Arenobufagin caused apoptosis in the non-small-cell lung cancer (NSCLC) cell line A549 by oxidative stress [39].…”

Diagnostic value of abnormal chromosome 3p genes in small‑cell lung cancer

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Establishment of a Nomogram-Based Prognostic Model (LASSO-COX Regression) for Predicting Progression-Free Survival of Primary Non-Small Cell Lung Cancer Patients Treated with Adjuvant Chinese Herbal Medicines Therapy: A Retrospective Study of Case Series

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