JK1 (FAM134B) represses cell migration in colon cancer: a functional study of a novel gene

Kasem, Kais; Sullivan, Elizabeth; Gopalan, Vinod; Salajegheh, Ali; Smith, Robert A.; Lam, Alfred King‐Yin

doi:10.1016/j.yexmp.2014.06.002

Cited by 29 publications

(41 citation statements)

References 12 publications

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“…The assay has a straightforward design, with readout being performed in two steps, involving the introduction of a non-covalently attached redox system. As FAM134B expression was correlated with cancer stages, pathogenesis and clinicopathological parameters of patients suffering from colorectal cancer, our method may help in easy and cost effective detection of FAM134B expression in cancer patients’ clinical samples 5–8, 11 . To achieve these, further studies with a larger series of clinical cohorts are needed for the functional validation of our current approach.…”

Section: Resultsmentioning

confidence: 98%

“…FAM134B protein is reported to function as a tumour suppressor and its’ changes in expression was associated with different pathological stages in patients with colorectal carcinomas 5 . Additionally, FAM134B expression was correlated with poor survival in colorectal cancer patients 6–8 . Thus, an accurate and specific detection of this protein with a simple method could be used as a liquid biopsy for monitoring its role in the progression or recurrence of colorectal cancer 9–13 .…”

Section: Introductionmentioning

confidence: 95%

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An electrochemical method for sensitive and rapid detection of FAM134B protein in colon cancer samples

Islam

Haque

Yadav

et al. 2017

Sci Rep

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Despite the excellent diagnostic applications of the current conventional immunoassay methods such as ELISA, immunostaining and Western blot for FAM134B detection, they are laborious, expensive and required a long turnaround time. Here, we report an electrochemical approach for rapid, sensitive, and specific detection of FAM134B protein in biological (colon cancer cell extracts) and clinical (serum) samples. The approach utilises a differential pulse voltammetry (DPV) in the presence of the [Fe(CN)6]3−/4− redox system to quantify the FAM134B protein in a two-step strategy that involves (i) initial attachment of FAM134B antibody on the surface of extravidin-modified screen-printed carbon electrode, and (ii) subsequent detection of FAM134B protein present in the biological/clinical samples. The assay system was able to detect FAM134B protein at a concentration down to 10 pg μL−1 in phosphate buffered saline (pH 7.4) with a good inter-assay reproducibility (% RSD = <8.64, n = 3). We found excellent sensitivity and specificity for the analysis of FAM134B protein in a panel of colon cancer cell lines and serum samples. Finally, the assay was further validated with ELISA method. We believe that our assay could potentially lead a low-cost alternative to conventional immunological assays for target antigens analysis in point-of-care applications.

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Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 95%

An electrochemical method for sensitive and rapid detection of FAM134B protein in colon cancer samples

Islam

Haque

Yadav

et al. 2017

Sci Rep

Self Cite

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show abstract

“…7d). In addition, FAM134B, a putative tumour suppressor60 was identified as a potential target of miR-31 (down 4.3±1.4-fold; mean±s.e.m. ; n =5 sets; Fig.…”

Section: Resultsmentioning

confidence: 99%

Cross-species identification of genomic drivers of squamous cell carcinoma development across preneoplastic intermediates

et al. 2016

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Cutaneous squamous cell carcinoma (cuSCC) comprises 15–20% of all skin cancers, accounting for over 700,000 cases in USA annually. Most cuSCC arise in association with a distinct precancerous lesion, the actinic keratosis (AK). To identify potential targets for molecularly targeted chemoprevention, here we perform integrated cross-species genomic analysis of cuSCC development through the preneoplastic AK stage using matched human samples and a solar ultraviolet radiation-driven Hairless mouse model. We identify the major transcriptional drivers of this progression sequence, showing that the key genomic changes in cuSCC development occur in the normal skin to AK transition. Our data validate the use of this ultraviolet radiation-driven mouse cuSCC model for cross-species analysis and demonstrate that cuSCC bears deep molecular similarities to multiple carcinogen-driven SCCs from diverse sites, suggesting that cuSCC may serve as an effective, accessible model for multiple SCC types and that common treatment and prevention strategies may be feasible.

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“…FAM134B gene encodes a cis‐Golgi transmembrane protein that controls its expression patterns and regulates apoptosis in gastrointestinal carcinomas and neurological diseases ,,. More recently, we have reported 37 mutations in different exons of FAM134B gene and its significant DNA copy number variations in ESCC tissues .…”

Section: Resultsmentioning

confidence: 93%

Electrochemical Detection of FAM134B Mutations in Oesophageal Cancer Based on DNA‐Gold Affinity Interactions

Haque

Islam

et al. 2017

Electroanalysis

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Inexpensive, simple and rapid DNA sensors capable of accurate and sensitive detection of cancer specific point mutations in DNA biomarkers are crucial for the routine screening of genetic mutations in cancer. Conventional approaches based on sequencing, mass spectroscopy, and fluorescence are highly effective, but they are tedious, slow and require labels and expensive equipment. Recent electrochemistry based approaches mostly rely on conventional DNA biosensing using recognition and transduction layers, and hence limited by the complicated steps of sensor fabrication associated with surface cleaning, self‐assembled monolayer formation, and target hybridization. Herein we report a relatively simple and inexpensive method for detecting point mutation in cancer by using the direct adsorption of purified DNA sequences onto an unmodified gold surface. The method relies on the base dependent affinity interaction of DNA with gold. Since the affinity interaction (adsorption) trend of DNA bases follows as adenine (A) > cytosine (C) > guanine (G)> thymine (T), two DNA sequences with different DNA base compositions (i. e., amplified mutated sequences will be distinctly different than its original sequence) will have different adsorption affinity towards gold. The amount of mutation sites on a DNA sequence is quantified by monitoring the electrochemical current as a function of the relative adsorption level of DNA samples onto a bare gold electrode. This method can successfully distinguish single point mutation in DNA from oesophageal cancer. We demonstrated the clinical utility of this approach by detecting different levels of mutations in tissue samples (n=9) taken from oesophageal cancer patients. Finally, the method was validated with High Resolution Melt (HRM) curve analysis and Sanger Sequencing.

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JK1 (FAM134B) represses cell migration in colon cancer: a functional study of a novel gene

Cited by 29 publications

References 12 publications

An electrochemical method for sensitive and rapid detection of FAM134B protein in colon cancer samples

An electrochemical method for sensitive and rapid detection of FAM134B protein in colon cancer samples

Cross-species identification of genomic drivers of squamous cell carcinoma development across preneoplastic intermediates

Electrochemical Detection of FAM134B Mutations in Oesophageal Cancer Based on DNA‐Gold Affinity Interactions

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