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Wang, Y.; R, Aun; Tse, Francis L. S.

doi:10.1023/a:1012174217220

Cited by 18 publications

(2 citation statements)

References 16 publications

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“…Similar results were obtained during the in vitro culture of human endothelial cells (HMEC‐1 line) in the presence of isolated soluble sericin from B. mori and A. pernyi at concentrations of 0.1 and 1.0 mg/ml [55]. Moreover, wound‐healing tests with creams containing sericin (8% w/v) did not induce hypersensitivity or other systemic responses in tests with rats in vivo [92]. Nevertheless, clinical evidence exists in humans that sericin can induce a strong allergic response [93,94], which is probably specific for distinct mammalian species (see also the next section).…”

Section: Toxicity Of Silk Materialssupporting

confidence: 64%

Silk‐based materials for biomedical applications

Leal‐Egaña

Scheibel

2010

Biotech and App Biochem

209

177

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Since the beginning of civilization, humans have exploited nature as an extraordinary source of materials for medical applications. Most natural materials comprise biopolymers such as nucleic acids and protein-polysaccharides. For biomedical applications, proteins such as collagens have been traditionally employed. Other proteins are silk fibres produced by arthropods (e.g. silkworms and spiders), which provide interesting mechanical properties and the absence of toxicity. Silks present almost all characteristics desirable for biomedical applications, but the research on the underlying proteins has only recently commenced. In the present review, we summarize the current research related to silk being used as a material for cell culture and tissue engineering, particularly focusing on cell-surface adherence, mechanical and textural properties, toxicity, immunogenicity and biodegradability.

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Section: Toxicity Of Silk Materialssupporting

confidence: 64%

Silk‐based materials for biomedical applications

Leal‐Egaña

Scheibel

2010

Biotech and App Biochem

209

177

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“…A modified version of a previously described perfusion model was used (40). Fasted male rats were anesthetized with pentobarbital (Narcoren, Merial GmbH, Halbergmoos, Germany, 60 mg/kg intraperitoneal).…”

Section: Measurement Of Glucose Absorption In Rat Small Intestinementioning

confidence: 99%

Tripeptides of RS1 (RSC1A1) Inhibit a Monosaccharide-dependent Exocytotic Pathway of Na+-d-Glucose Cotransporter SGLT1 with High Affinity

Vernaleken

Veyhl

Gorboulev

et al. 2007

Journal of Biological Chemistry

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؉ -peptide cotransporter PepT1 that is colocated with SGLT1 in small intestinal enterocytes. Using coexpression of SGLT1 and PepT1 in Xenopus oocytes or polarized Caco-2 cells that contain both transporters we demonstrated that the tripeptides were effective when applied to the extracellular compartment. After a 1-h perfusion of intact rat small intestine with QSP, glucose absorption was reduced by 30%. The data indicate that orally applied tripeptides can be used to down-regulate small intestinal glucose absorption, e.g. in diabetes mellitus.

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Absorption and disposition of a tripeptoid and a tetrapeptide in the rat

Wang¹,

Lin²,

Tullman³

et al. 1999

Biopharm. Drug Dispos.

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The present study is concerned with the absorption and disposition of a tripeptoid (N‐substituted glycine derivative) and a tetrapeptide in the rat. The two compounds have similar backbone structures but differ with respect to the presence or absence of peptide bond. [3H]tripeptoid and [3H]tetrapeptide were administered orally (30 mg kg−1) and intravenously (i.v.) (30 or 3 mg kg−1) to Sprague Dawley rats. Blood, urine and feces were collected at designated times for radioactivity and parent drug analysis. The intestinal absorptive clearances of the tripeptoid and tetrapeptide were studied using an in situ rat intestinal perfusion model. The octanol/water partition coefficient of these two compounds was also determined. The results showed that the peptoid and peptide have similar absorptive clearance and octanol/water partitioning, but different in vivo absorption and disposition characteristics. The absorptive clearances of the tripeptoid and tetrapeptide were 6.7 and 4.8×10−4 mL min−1 cm−1, respectively, and the corresponding octanol/water partition coefficients were 0.39 and 0.30. The extent of oral absorption of the tripeptoid was only 3–8%, consistent with its low absorptive clearance. In contrast, the apparent absorption of the tetrapeptide was >75% of the radioactive dose. The peptide was completely metabolized within 2 h after an i.v. dose, whereas the peptoid was stable in blood and was primarily eliminated in feces as intact drug. In conclusion, the difference in in vivo absorption and disposition between the peptoid and peptide was apparently due to the presence or absence of a peptide bond. The tetrapeptide was subject to rapid metabolism in the body. Its relatively high absorption appeared to represent the absorption of metabolized radioactive fragments. The peptoid appears to have advantages over the peptide in term of metabolic stability, but its low oral absorption and rapid biliary excretion present additional challenges in the selection of an optimal drug candidate. Copyright © 1999 John Wiley & Sons, Ltd.

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Untitled

Cited by 18 publications

References 16 publications

Silk‐based materials for biomedical applications

Silk‐based materials for biomedical applications

Tripeptides of RS1 (RSC1A1) Inhibit a Monosaccharide-dependent Exocytotic Pathway of Na+-d-Glucose Cotransporter SGLT1 with High Affinity

Absorption and disposition of a tripeptoid and a tetrapeptide in the rat

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